Limits...
The IDA/IDA-LIKE and PIP/PIP-LIKE gene families in Arabidopsis: phylogenetic relationship, expression patterns, and transcriptional effect of the PIPL3 peptide.

Vie AK, Najafi J, Liu B, Winge P, Butenko MA, Hornslien KS, Kumpf R, Aalen RB, Bones AM, Brembu T - J. Exp. Bot. (2015)

Bottom Line: Here we present three novel members of the IDL subfamily and show that two of them are strongly and rapidly induced by different biotic and abiotic stresses.Expression patterns of the IDA/IDL and PIP/PIPL genes were analysed using in silico data, qRT-PCR and GUS promoter lines.Transcriptomic responses to PIPL3 peptide treatment suggested a role in regulation of biotic stress responses and cell wall modification.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.


Related in: MedlinePlus

Stress-induced expression of IDA/IDL and PIP/PIPL genes based on qRT-PCR. Two-week-old plants were subjected to the following treatments for 6h unless otherwise specified: 10 μg/ml chitin (1h), 150mM NaCl, 10 μg/ml CHX and 15 μg/ml anisomycin. Infestation with Brevicoryne brassicae was done on 23-day-old plants, and rosette leaves were harvested after 72h (34). n=3. The relative expression (log2 ratios) values between treated and mock-treated samples are given next to the colour scale. Statistical differences between treated and mock-treated plants are indicated, where a diagonal line indicates no significance (REST analysis; P>0.05), and no diagonal line indicates significant difference (REST analysis; P<0.05). Brv, Brevicoryne brassicae; Salt, NaCl; CHX, cycloheximide; ANI, anisomycin.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4526919&req=5

Figure 8: Stress-induced expression of IDA/IDL and PIP/PIPL genes based on qRT-PCR. Two-week-old plants were subjected to the following treatments for 6h unless otherwise specified: 10 μg/ml chitin (1h), 150mM NaCl, 10 μg/ml CHX and 15 μg/ml anisomycin. Infestation with Brevicoryne brassicae was done on 23-day-old plants, and rosette leaves were harvested after 72h (34). n=3. The relative expression (log2 ratios) values between treated and mock-treated samples are given next to the colour scale. Statistical differences between treated and mock-treated plants are indicated, where a diagonal line indicates no significance (REST analysis; P>0.05), and no diagonal line indicates significant difference (REST analysis; P<0.05). Brv, Brevicoryne brassicae; Salt, NaCl; CHX, cycloheximide; ANI, anisomycin.

Mentions: In order to complement the in silico data, Arabidopsis Col-0 seedlings were subjected to various biotic and abiotic treatments and a qRT-PCR analysis was performed to obtain information on all IDA/IDL and PIP/PIPL members (Fig. 8). Treatments with the elicitor chitin induced several of the genes, with the highest log2 ratio observed for IDL6 and PIPL6. The aphid Brevicoryne brassicae, which is a specialist on Brassicaceae species, was used as biotic treatment, and induced the expression of a large subset of the genes. Ten genes were up-regulated with log2>1; among these were IDA, PIP2, PIP3 and PIPL1. Salt stress induced eight of the genes log2>1, confirming the in silico data.


The IDA/IDA-LIKE and PIP/PIP-LIKE gene families in Arabidopsis: phylogenetic relationship, expression patterns, and transcriptional effect of the PIPL3 peptide.

Vie AK, Najafi J, Liu B, Winge P, Butenko MA, Hornslien KS, Kumpf R, Aalen RB, Bones AM, Brembu T - J. Exp. Bot. (2015)

Stress-induced expression of IDA/IDL and PIP/PIPL genes based on qRT-PCR. Two-week-old plants were subjected to the following treatments for 6h unless otherwise specified: 10 μg/ml chitin (1h), 150mM NaCl, 10 μg/ml CHX and 15 μg/ml anisomycin. Infestation with Brevicoryne brassicae was done on 23-day-old plants, and rosette leaves were harvested after 72h (34). n=3. The relative expression (log2 ratios) values between treated and mock-treated samples are given next to the colour scale. Statistical differences between treated and mock-treated plants are indicated, where a diagonal line indicates no significance (REST analysis; P>0.05), and no diagonal line indicates significant difference (REST analysis; P<0.05). Brv, Brevicoryne brassicae; Salt, NaCl; CHX, cycloheximide; ANI, anisomycin.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526919&req=5

Figure 8: Stress-induced expression of IDA/IDL and PIP/PIPL genes based on qRT-PCR. Two-week-old plants were subjected to the following treatments for 6h unless otherwise specified: 10 μg/ml chitin (1h), 150mM NaCl, 10 μg/ml CHX and 15 μg/ml anisomycin. Infestation with Brevicoryne brassicae was done on 23-day-old plants, and rosette leaves were harvested after 72h (34). n=3. The relative expression (log2 ratios) values between treated and mock-treated samples are given next to the colour scale. Statistical differences between treated and mock-treated plants are indicated, where a diagonal line indicates no significance (REST analysis; P>0.05), and no diagonal line indicates significant difference (REST analysis; P<0.05). Brv, Brevicoryne brassicae; Salt, NaCl; CHX, cycloheximide; ANI, anisomycin.
Mentions: In order to complement the in silico data, Arabidopsis Col-0 seedlings were subjected to various biotic and abiotic treatments and a qRT-PCR analysis was performed to obtain information on all IDA/IDL and PIP/PIPL members (Fig. 8). Treatments with the elicitor chitin induced several of the genes, with the highest log2 ratio observed for IDL6 and PIPL6. The aphid Brevicoryne brassicae, which is a specialist on Brassicaceae species, was used as biotic treatment, and induced the expression of a large subset of the genes. Ten genes were up-regulated with log2>1; among these were IDA, PIP2, PIP3 and PIPL1. Salt stress induced eight of the genes log2>1, confirming the in silico data.

Bottom Line: Here we present three novel members of the IDL subfamily and show that two of them are strongly and rapidly induced by different biotic and abiotic stresses.Expression patterns of the IDA/IDL and PIP/PIPL genes were analysed using in silico data, qRT-PCR and GUS promoter lines.Transcriptomic responses to PIPL3 peptide treatment suggested a role in regulation of biotic stress responses and cell wall modification.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.


Related in: MedlinePlus