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The IDA/IDA-LIKE and PIP/PIP-LIKE gene families in Arabidopsis: phylogenetic relationship, expression patterns, and transcriptional effect of the PIPL3 peptide.

Vie AK, Najafi J, Liu B, Winge P, Butenko MA, Hornslien KS, Kumpf R, Aalen RB, Bones AM, Brembu T - J. Exp. Bot. (2015)

Bottom Line: Here we present three novel members of the IDL subfamily and show that two of them are strongly and rapidly induced by different biotic and abiotic stresses.Expression patterns of the IDA/IDL and PIP/PIPL genes were analysed using in silico data, qRT-PCR and GUS promoter lines.Transcriptomic responses to PIPL3 peptide treatment suggested a role in regulation of biotic stress responses and cell wall modification.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.


Related in: MedlinePlus

Stress-induced expression of IDA/IDL and PIP/PIPL genes based on in silico data. (A) Abiotic stress. Cold root, root tissue collected from cold-treated seedlings (continuous 4°C); salt root, root tissue collected from salt-treated seedlings (150mM NaCl); UV root/shoot, root and shoot tissue collected from UV-treated seedlings (15min treatment in UV-B field). (B) Biotic stress. Pseudomonas syringae half-leaf infiltration: C, control (10mM MgCl2); A, avirulent P. syringae ES4326 avrRPt2; V, virulent P. syringae ES4326. Botrytis cinerea treatments: C, control (potato dextrose broth); T, treated (B. cinerea 5×105 spores/ml). Phytophthora infestans treatments: C, control (water); T, treated (Phytophthora infestans 106 spores/ml). (C) Cycloheximide (CHX) treatment (10 μM CHX, 3h). All data was obtained from the Arabidopsis eFP browser at the Bio-Array Resource database (Winter et al., 2007). The arithmetic expression values are given next to the colour scale.
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Figure 7: Stress-induced expression of IDA/IDL and PIP/PIPL genes based on in silico data. (A) Abiotic stress. Cold root, root tissue collected from cold-treated seedlings (continuous 4°C); salt root, root tissue collected from salt-treated seedlings (150mM NaCl); UV root/shoot, root and shoot tissue collected from UV-treated seedlings (15min treatment in UV-B field). (B) Biotic stress. Pseudomonas syringae half-leaf infiltration: C, control (10mM MgCl2); A, avirulent P. syringae ES4326 avrRPt2; V, virulent P. syringae ES4326. Botrytis cinerea treatments: C, control (potato dextrose broth); T, treated (B. cinerea 5×105 spores/ml). Phytophthora infestans treatments: C, control (water); T, treated (Phytophthora infestans 106 spores/ml). (C) Cycloheximide (CHX) treatment (10 μM CHX, 3h). All data was obtained from the Arabidopsis eFP browser at the Bio-Array Resource database (Winter et al., 2007). The arithmetic expression values are given next to the colour scale.

Mentions: In general, the members of the IDA/IDL and PIP/PIPL families were found to be only weakly expressed under normal growth and development, so the response of IDA/IDL and PIP/PIPL genes to different abiotic and biotic stresses were therefore investigated (Fig. 7). Figure 7A summarizes the in silico analysis of abiotic stresses (Kilian et al., 2007). Cold stress induces expression of IDL7, PIP1 and PIP3 in roots, whereas UV induces eight out of 11 of the IDA/IDL/PIP/PIPL genes present on the Affymetrix ATH1 microarrays. The highest expression is observed in roots during salt stress. IDA, IDL1, IDL7, PIP1 and PIP3 are especially highly induced upon such stress, with expression levels up-regulated 500–1000 times compared to the control. PIPL1 is not expressed during any of the specified treatments. Biotic stress and treatments with elicitors (Fig. 7B; Supplementary Fig. S3) induces fewer genes than abiotic stress. Treatments with both virulent and avirulent strains of the biotrophic pathogen Pseudomonas syringae induce IDL6 and IDL7 expression. It should be noticed that expression of IDL6 is induced 1h after treatments with the pathogen-derived elicitors flg22, HrpZ and NPP1. PIP1 is up-regulated upon treatment with NPP1 and HrpZ (Supplementary Fig. S3). The necrotrophic pathogens Botrytis cinerea and Phytophtora infestans both induce expression of PIP2 and PIP3. This is in accordance with data recently published by Hou et al. (2014), showing that PIP1, PIP2 and PIP3 are involved in amplification of the immune response. IDL6 and IDL7 are in general induced at earlier time points than the rest of the genes (Fig. 7; Supplementary Fig. S3). Transcripts of IDL6 and IDL7 are detected as early as 15min post UV exposure, while PIPL2 and PIP3 are detected 30min (PIPL2) and 3h (PIP3) after exposure (Fig. 7A). Data obtained from Genevestigator (Hruz et al., 2008) confirmed these results (Supplementary Dataset S2).


The IDA/IDA-LIKE and PIP/PIP-LIKE gene families in Arabidopsis: phylogenetic relationship, expression patterns, and transcriptional effect of the PIPL3 peptide.

Vie AK, Najafi J, Liu B, Winge P, Butenko MA, Hornslien KS, Kumpf R, Aalen RB, Bones AM, Brembu T - J. Exp. Bot. (2015)

Stress-induced expression of IDA/IDL and PIP/PIPL genes based on in silico data. (A) Abiotic stress. Cold root, root tissue collected from cold-treated seedlings (continuous 4°C); salt root, root tissue collected from salt-treated seedlings (150mM NaCl); UV root/shoot, root and shoot tissue collected from UV-treated seedlings (15min treatment in UV-B field). (B) Biotic stress. Pseudomonas syringae half-leaf infiltration: C, control (10mM MgCl2); A, avirulent P. syringae ES4326 avrRPt2; V, virulent P. syringae ES4326. Botrytis cinerea treatments: C, control (potato dextrose broth); T, treated (B. cinerea 5×105 spores/ml). Phytophthora infestans treatments: C, control (water); T, treated (Phytophthora infestans 106 spores/ml). (C) Cycloheximide (CHX) treatment (10 μM CHX, 3h). All data was obtained from the Arabidopsis eFP browser at the Bio-Array Resource database (Winter et al., 2007). The arithmetic expression values are given next to the colour scale.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 7: Stress-induced expression of IDA/IDL and PIP/PIPL genes based on in silico data. (A) Abiotic stress. Cold root, root tissue collected from cold-treated seedlings (continuous 4°C); salt root, root tissue collected from salt-treated seedlings (150mM NaCl); UV root/shoot, root and shoot tissue collected from UV-treated seedlings (15min treatment in UV-B field). (B) Biotic stress. Pseudomonas syringae half-leaf infiltration: C, control (10mM MgCl2); A, avirulent P. syringae ES4326 avrRPt2; V, virulent P. syringae ES4326. Botrytis cinerea treatments: C, control (potato dextrose broth); T, treated (B. cinerea 5×105 spores/ml). Phytophthora infestans treatments: C, control (water); T, treated (Phytophthora infestans 106 spores/ml). (C) Cycloheximide (CHX) treatment (10 μM CHX, 3h). All data was obtained from the Arabidopsis eFP browser at the Bio-Array Resource database (Winter et al., 2007). The arithmetic expression values are given next to the colour scale.
Mentions: In general, the members of the IDA/IDL and PIP/PIPL families were found to be only weakly expressed under normal growth and development, so the response of IDA/IDL and PIP/PIPL genes to different abiotic and biotic stresses were therefore investigated (Fig. 7). Figure 7A summarizes the in silico analysis of abiotic stresses (Kilian et al., 2007). Cold stress induces expression of IDL7, PIP1 and PIP3 in roots, whereas UV induces eight out of 11 of the IDA/IDL/PIP/PIPL genes present on the Affymetrix ATH1 microarrays. The highest expression is observed in roots during salt stress. IDA, IDL1, IDL7, PIP1 and PIP3 are especially highly induced upon such stress, with expression levels up-regulated 500–1000 times compared to the control. PIPL1 is not expressed during any of the specified treatments. Biotic stress and treatments with elicitors (Fig. 7B; Supplementary Fig. S3) induces fewer genes than abiotic stress. Treatments with both virulent and avirulent strains of the biotrophic pathogen Pseudomonas syringae induce IDL6 and IDL7 expression. It should be noticed that expression of IDL6 is induced 1h after treatments with the pathogen-derived elicitors flg22, HrpZ and NPP1. PIP1 is up-regulated upon treatment with NPP1 and HrpZ (Supplementary Fig. S3). The necrotrophic pathogens Botrytis cinerea and Phytophtora infestans both induce expression of PIP2 and PIP3. This is in accordance with data recently published by Hou et al. (2014), showing that PIP1, PIP2 and PIP3 are involved in amplification of the immune response. IDL6 and IDL7 are in general induced at earlier time points than the rest of the genes (Fig. 7; Supplementary Fig. S3). Transcripts of IDL6 and IDL7 are detected as early as 15min post UV exposure, while PIPL2 and PIP3 are detected 30min (PIPL2) and 3h (PIP3) after exposure (Fig. 7A). Data obtained from Genevestigator (Hruz et al., 2008) confirmed these results (Supplementary Dataset S2).

Bottom Line: Here we present three novel members of the IDL subfamily and show that two of them are strongly and rapidly induced by different biotic and abiotic stresses.Expression patterns of the IDA/IDL and PIP/PIPL genes were analysed using in silico data, qRT-PCR and GUS promoter lines.Transcriptomic responses to PIPL3 peptide treatment suggested a role in regulation of biotic stress responses and cell wall modification.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.


Related in: MedlinePlus