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The IDA/IDA-LIKE and PIP/PIP-LIKE gene families in Arabidopsis: phylogenetic relationship, expression patterns, and transcriptional effect of the PIPL3 peptide.

Vie AK, Najafi J, Liu B, Winge P, Butenko MA, Hornslien KS, Kumpf R, Aalen RB, Bones AM, Brembu T - J. Exp. Bot. (2015)

Bottom Line: Here we present three novel members of the IDL subfamily and show that two of them are strongly and rapidly induced by different biotic and abiotic stresses.Expression patterns of the IDA/IDL and PIP/PIPL genes were analysed using in silico data, qRT-PCR and GUS promoter lines.Transcriptomic responses to PIPL3 peptide treatment suggested a role in regulation of biotic stress responses and cell wall modification.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.


Related in: MedlinePlus

Histochemical analysis of promoter:GUS expression using the promoters of IDA and IDL1 to IDL5 genes. (A) pIDA:GUS is expressed during lateral root emergence in endodermis (63× magnification), cortex and epidermis cells; pIDL1:GUS in columella root cap cells (40×); pIDL3:GUS and pIDL4:GUS in the vasculature and pIDL5:GUS next to the hypocotyl (20×). (B) pIDL2:GUS and pIDL3:GUS are, in contrast to pIDL1:GUS, expressed in the cotyledons 3 d after germination (40×). (C) pIDL3:GUS, pIDL4:GUS and pIDL5:GUS are expressed in the vasculature of the first true leaves, including the hydathodes (20×). (D) pIDL4:GUS is expressed in the guard cells (40×); (E) pIDL4:GUS and IDL5:GUS are expressed in the shoot apical meristem (40×), (F) pIDL2:GUS, pIDL3:GUS and pIDL4:GUS are expressed in the vasculature of flowers at developmental stage 6.0F (20×). (G) pIDL1:GUS, pIDL2:GUS and pIDL3:GUS are expressed in the vasculature in the abscission zone region after the floral organs have been shed (20×). (H) pIDL2:GUS and pIDL3:GUS are in contrast to pIDL1:GUS expressed in vestigial abscission zones at the base of pedicels and branches (20×).
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Figure 6: Histochemical analysis of promoter:GUS expression using the promoters of IDA and IDL1 to IDL5 genes. (A) pIDA:GUS is expressed during lateral root emergence in endodermis (63× magnification), cortex and epidermis cells; pIDL1:GUS in columella root cap cells (40×); pIDL3:GUS and pIDL4:GUS in the vasculature and pIDL5:GUS next to the hypocotyl (20×). (B) pIDL2:GUS and pIDL3:GUS are, in contrast to pIDL1:GUS, expressed in the cotyledons 3 d after germination (40×). (C) pIDL3:GUS, pIDL4:GUS and pIDL5:GUS are expressed in the vasculature of the first true leaves, including the hydathodes (20×). (D) pIDL4:GUS is expressed in the guard cells (40×); (E) pIDL4:GUS and IDL5:GUS are expressed in the shoot apical meristem (40×), (F) pIDL2:GUS, pIDL3:GUS and pIDL4:GUS are expressed in the vasculature of flowers at developmental stage 6.0F (20×). (G) pIDL1:GUS, pIDL2:GUS and pIDL3:GUS are expressed in the vasculature in the abscission zone region after the floral organs have been shed (20×). (H) pIDL2:GUS and pIDL3:GUS are in contrast to pIDL1:GUS expressed in vestigial abscission zones at the base of pedicels and branches (20×).

Mentions: To further investigate the expression pattern of the different IDL genes during early Arabidopsis development, plants expressing promoter:GUS reporter constructs for IDA and IDL genes (pIDA/pIDL:GUS) were investigated from germination up until 14 d after germination (Supplementary Table S3). pIDA:GUS expression was observed in the cortex and epidermal cells overlaying the lateral root primordia in accordance with the recently reported function in cell separation allowing lateral root emergence (Fig. 6A; Kumpf et al., 2013). pIDL1:GUS had a specific pattern of expression in the columella root cap cells of the primary root (Fig. 6A), where cells undergo cell separation during root cap sloughing, which allows the primary root to penetrate the soil (del Campillo et al., 2004). After germination, pIDL2:GUS, pIDL4:GUS and pIDL5:GUS were expressed in the vascular tissue of the primary and lateral roots (Fig. 6A). Consistent with the expression in leaves identified by qRT-PCR (Fig. 5), the pIDL:GUS constructs were all expressed in the vascular tissue of the expanding cotyledons and/or primary leaves (Fig. 6B, C), and IDL4 expression was also observed in the guard cells (Fig. 6D) (Stenvik et al., 2008). The IDL promoter:GUS constructs were all expressed in the shoot apical meristem region, here represented by IDL4 and IDL5 in Fig. 6E. Expression was then investigated in various tissues at later developmental stages, as described by Boyes et al. (2001). Previous studies have shown that IDA, IDL2, IDL3 and IDL4 are expressed in the floral abscission zone (Stenvik et al., 2008). None of the genes were expressed in the abscission zone region at the time points investigated by qRT-PCR, although expression was detected in the vasculature of the developing floral organs (Fig. 6F). However, IDL1, IDL2 and IDL3 showed expression in the abscission zone at later stages, when the floral organs are already abscised (Fig. 6G). In addition, the same genes showed expression in the vestigial abscission zones of the pedicel region (Fig. 6H).


The IDA/IDA-LIKE and PIP/PIP-LIKE gene families in Arabidopsis: phylogenetic relationship, expression patterns, and transcriptional effect of the PIPL3 peptide.

Vie AK, Najafi J, Liu B, Winge P, Butenko MA, Hornslien KS, Kumpf R, Aalen RB, Bones AM, Brembu T - J. Exp. Bot. (2015)

Histochemical analysis of promoter:GUS expression using the promoters of IDA and IDL1 to IDL5 genes. (A) pIDA:GUS is expressed during lateral root emergence in endodermis (63× magnification), cortex and epidermis cells; pIDL1:GUS in columella root cap cells (40×); pIDL3:GUS and pIDL4:GUS in the vasculature and pIDL5:GUS next to the hypocotyl (20×). (B) pIDL2:GUS and pIDL3:GUS are, in contrast to pIDL1:GUS, expressed in the cotyledons 3 d after germination (40×). (C) pIDL3:GUS, pIDL4:GUS and pIDL5:GUS are expressed in the vasculature of the first true leaves, including the hydathodes (20×). (D) pIDL4:GUS is expressed in the guard cells (40×); (E) pIDL4:GUS and IDL5:GUS are expressed in the shoot apical meristem (40×), (F) pIDL2:GUS, pIDL3:GUS and pIDL4:GUS are expressed in the vasculature of flowers at developmental stage 6.0F (20×). (G) pIDL1:GUS, pIDL2:GUS and pIDL3:GUS are expressed in the vasculature in the abscission zone region after the floral organs have been shed (20×). (H) pIDL2:GUS and pIDL3:GUS are in contrast to pIDL1:GUS expressed in vestigial abscission zones at the base of pedicels and branches (20×).
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Figure 6: Histochemical analysis of promoter:GUS expression using the promoters of IDA and IDL1 to IDL5 genes. (A) pIDA:GUS is expressed during lateral root emergence in endodermis (63× magnification), cortex and epidermis cells; pIDL1:GUS in columella root cap cells (40×); pIDL3:GUS and pIDL4:GUS in the vasculature and pIDL5:GUS next to the hypocotyl (20×). (B) pIDL2:GUS and pIDL3:GUS are, in contrast to pIDL1:GUS, expressed in the cotyledons 3 d after germination (40×). (C) pIDL3:GUS, pIDL4:GUS and pIDL5:GUS are expressed in the vasculature of the first true leaves, including the hydathodes (20×). (D) pIDL4:GUS is expressed in the guard cells (40×); (E) pIDL4:GUS and IDL5:GUS are expressed in the shoot apical meristem (40×), (F) pIDL2:GUS, pIDL3:GUS and pIDL4:GUS are expressed in the vasculature of flowers at developmental stage 6.0F (20×). (G) pIDL1:GUS, pIDL2:GUS and pIDL3:GUS are expressed in the vasculature in the abscission zone region after the floral organs have been shed (20×). (H) pIDL2:GUS and pIDL3:GUS are in contrast to pIDL1:GUS expressed in vestigial abscission zones at the base of pedicels and branches (20×).
Mentions: To further investigate the expression pattern of the different IDL genes during early Arabidopsis development, plants expressing promoter:GUS reporter constructs for IDA and IDL genes (pIDA/pIDL:GUS) were investigated from germination up until 14 d after germination (Supplementary Table S3). pIDA:GUS expression was observed in the cortex and epidermal cells overlaying the lateral root primordia in accordance with the recently reported function in cell separation allowing lateral root emergence (Fig. 6A; Kumpf et al., 2013). pIDL1:GUS had a specific pattern of expression in the columella root cap cells of the primary root (Fig. 6A), where cells undergo cell separation during root cap sloughing, which allows the primary root to penetrate the soil (del Campillo et al., 2004). After germination, pIDL2:GUS, pIDL4:GUS and pIDL5:GUS were expressed in the vascular tissue of the primary and lateral roots (Fig. 6A). Consistent with the expression in leaves identified by qRT-PCR (Fig. 5), the pIDL:GUS constructs were all expressed in the vascular tissue of the expanding cotyledons and/or primary leaves (Fig. 6B, C), and IDL4 expression was also observed in the guard cells (Fig. 6D) (Stenvik et al., 2008). The IDL promoter:GUS constructs were all expressed in the shoot apical meristem region, here represented by IDL4 and IDL5 in Fig. 6E. Expression was then investigated in various tissues at later developmental stages, as described by Boyes et al. (2001). Previous studies have shown that IDA, IDL2, IDL3 and IDL4 are expressed in the floral abscission zone (Stenvik et al., 2008). None of the genes were expressed in the abscission zone region at the time points investigated by qRT-PCR, although expression was detected in the vasculature of the developing floral organs (Fig. 6F). However, IDL1, IDL2 and IDL3 showed expression in the abscission zone at later stages, when the floral organs are already abscised (Fig. 6G). In addition, the same genes showed expression in the vestigial abscission zones of the pedicel region (Fig. 6H).

Bottom Line: Here we present three novel members of the IDL subfamily and show that two of them are strongly and rapidly induced by different biotic and abiotic stresses.Expression patterns of the IDA/IDL and PIP/PIPL genes were analysed using in silico data, qRT-PCR and GUS promoter lines.Transcriptomic responses to PIPL3 peptide treatment suggested a role in regulation of biotic stress responses and cell wall modification.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.


Related in: MedlinePlus