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The IDA/IDA-LIKE and PIP/PIP-LIKE gene families in Arabidopsis: phylogenetic relationship, expression patterns, and transcriptional effect of the PIPL3 peptide.

Vie AK, Najafi J, Liu B, Winge P, Butenko MA, Hornslien KS, Kumpf R, Aalen RB, Bones AM, Brembu T - J. Exp. Bot. (2015)

Bottom Line: Here we present three novel members of the IDL subfamily and show that two of them are strongly and rapidly induced by different biotic and abiotic stresses.Expression patterns of the IDA/IDL and PIP/PIPL genes were analysed using in silico data, qRT-PCR and GUS promoter lines.Transcriptomic responses to PIPL3 peptide treatment suggested a role in regulation of biotic stress responses and cell wall modification.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.


Related in: MedlinePlus

Developmental expression patterns of IDA/IDL and PIP/PIPL genes based on in silico data. (A) Expression in different vegetative tissues during development. (B) Expression during embryo and seed development (Casson et al., 2005). All data was obtained from the Arabidopsis eFP browser at the Bio-Array Resource database (Winter et al., 2007). The arithmetic expression values are given next to the colour scale. Hyp, hypocotyl; Cot, cotyledon; 2RL, second rosette leaf; 6RL, sixth rosette leaf; 10RL, tenth rosette leaf; SCL, senescing leaves; ST, stem; FL12, flower stage 12; SQ4, silique position 4; SQ10, silique position 10; G-Ap, globular stage apical; G-Ba, globular stage basal; H-C, heart stage cotyledon; H-R, heart stage root; T-C, torpedo stage cotyledon; T-R, torpedo stage root; T-M, torpedo stage meristem; T-Ap, torpedo stage apical; T-Ba, torpedo stage basal; SC-PG, seed coat preglobular stage; SC-G, seed coat globular stage; SC-H, seed coat heart stage; SC-LC, seed coat linear cotyledon.
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Figure 4: Developmental expression patterns of IDA/IDL and PIP/PIPL genes based on in silico data. (A) Expression in different vegetative tissues during development. (B) Expression during embryo and seed development (Casson et al., 2005). All data was obtained from the Arabidopsis eFP browser at the Bio-Array Resource database (Winter et al., 2007). The arithmetic expression values are given next to the colour scale. Hyp, hypocotyl; Cot, cotyledon; 2RL, second rosette leaf; 6RL, sixth rosette leaf; 10RL, tenth rosette leaf; SCL, senescing leaves; ST, stem; FL12, flower stage 12; SQ4, silique position 4; SQ10, silique position 10; G-Ap, globular stage apical; G-Ba, globular stage basal; H-C, heart stage cotyledon; H-R, heart stage root; T-C, torpedo stage cotyledon; T-R, torpedo stage root; T-M, torpedo stage meristem; T-Ap, torpedo stage apical; T-Ba, torpedo stage basal; SC-PG, seed coat preglobular stage; SC-G, seed coat globular stage; SC-H, seed coat heart stage; SC-LC, seed coat linear cotyledon.

Mentions: Since the expression pattern of a gene may provide an indication of its function, we conducted an in silico analysis of the transcription levels of the genes included on the Affymetrix ATH1 microarrays (IDA, IDL1, IDL6, IDL7, PIP1-PIP3 and PIPL1-PIPL3) during plant development (Schmid et al., 2005), obtaining data from the eFP Browser (Winter et al., 2007). These genes are expressed at low levels both at post-germination stages and during embryogenesis (Fig. 4). However, IDL1, IDL6, PIP1 and PIPL3 are expressed in several embryonic tissues [data obtained from Casson et al. (2005)], with the highest expression found in root primordia during the torpedo stage. PIP1 is also expressed in basal tissue during the globular stage. PIPL1 is the most strongly expressed gene during seed development. In contrast to the rest of the genes, PIPL1 is not expressed in the embryo, but shows high expression levels in the seed coat during early stages of seed development.


The IDA/IDA-LIKE and PIP/PIP-LIKE gene families in Arabidopsis: phylogenetic relationship, expression patterns, and transcriptional effect of the PIPL3 peptide.

Vie AK, Najafi J, Liu B, Winge P, Butenko MA, Hornslien KS, Kumpf R, Aalen RB, Bones AM, Brembu T - J. Exp. Bot. (2015)

Developmental expression patterns of IDA/IDL and PIP/PIPL genes based on in silico data. (A) Expression in different vegetative tissues during development. (B) Expression during embryo and seed development (Casson et al., 2005). All data was obtained from the Arabidopsis eFP browser at the Bio-Array Resource database (Winter et al., 2007). The arithmetic expression values are given next to the colour scale. Hyp, hypocotyl; Cot, cotyledon; 2RL, second rosette leaf; 6RL, sixth rosette leaf; 10RL, tenth rosette leaf; SCL, senescing leaves; ST, stem; FL12, flower stage 12; SQ4, silique position 4; SQ10, silique position 10; G-Ap, globular stage apical; G-Ba, globular stage basal; H-C, heart stage cotyledon; H-R, heart stage root; T-C, torpedo stage cotyledon; T-R, torpedo stage root; T-M, torpedo stage meristem; T-Ap, torpedo stage apical; T-Ba, torpedo stage basal; SC-PG, seed coat preglobular stage; SC-G, seed coat globular stage; SC-H, seed coat heart stage; SC-LC, seed coat linear cotyledon.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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Figure 4: Developmental expression patterns of IDA/IDL and PIP/PIPL genes based on in silico data. (A) Expression in different vegetative tissues during development. (B) Expression during embryo and seed development (Casson et al., 2005). All data was obtained from the Arabidopsis eFP browser at the Bio-Array Resource database (Winter et al., 2007). The arithmetic expression values are given next to the colour scale. Hyp, hypocotyl; Cot, cotyledon; 2RL, second rosette leaf; 6RL, sixth rosette leaf; 10RL, tenth rosette leaf; SCL, senescing leaves; ST, stem; FL12, flower stage 12; SQ4, silique position 4; SQ10, silique position 10; G-Ap, globular stage apical; G-Ba, globular stage basal; H-C, heart stage cotyledon; H-R, heart stage root; T-C, torpedo stage cotyledon; T-R, torpedo stage root; T-M, torpedo stage meristem; T-Ap, torpedo stage apical; T-Ba, torpedo stage basal; SC-PG, seed coat preglobular stage; SC-G, seed coat globular stage; SC-H, seed coat heart stage; SC-LC, seed coat linear cotyledon.
Mentions: Since the expression pattern of a gene may provide an indication of its function, we conducted an in silico analysis of the transcription levels of the genes included on the Affymetrix ATH1 microarrays (IDA, IDL1, IDL6, IDL7, PIP1-PIP3 and PIPL1-PIPL3) during plant development (Schmid et al., 2005), obtaining data from the eFP Browser (Winter et al., 2007). These genes are expressed at low levels both at post-germination stages and during embryogenesis (Fig. 4). However, IDL1, IDL6, PIP1 and PIPL3 are expressed in several embryonic tissues [data obtained from Casson et al. (2005)], with the highest expression found in root primordia during the torpedo stage. PIP1 is also expressed in basal tissue during the globular stage. PIPL1 is the most strongly expressed gene during seed development. In contrast to the rest of the genes, PIPL1 is not expressed in the embryo, but shows high expression levels in the seed coat during early stages of seed development.

Bottom Line: Here we present three novel members of the IDL subfamily and show that two of them are strongly and rapidly induced by different biotic and abiotic stresses.Expression patterns of the IDA/IDL and PIP/PIPL genes were analysed using in silico data, qRT-PCR and GUS promoter lines.Transcriptomic responses to PIPL3 peptide treatment suggested a role in regulation of biotic stress responses and cell wall modification.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.


Related in: MedlinePlus