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The IDA/IDA-LIKE and PIP/PIP-LIKE gene families in Arabidopsis: phylogenetic relationship, expression patterns, and transcriptional effect of the PIPL3 peptide.

Vie AK, Najafi J, Liu B, Winge P, Butenko MA, Hornslien KS, Kumpf R, Aalen RB, Bones AM, Brembu T - J. Exp. Bot. (2015)

Bottom Line: Furthermore, we provide data that the recently identified PAMP-INDUCED SECRETED PEPTIDE (PIP) and PIP-LIKE (PIPL) peptides, which show similarity to the IDL and C-TERMINALLY ENCODED PEPTIDE (CEP) peptides, are not only involved in innate immune response in Arabidopsis but are also induced by abiotic stress.Expression patterns of the IDA/IDL and PIP/PIPL genes were analysed using in silico data, qRT-PCR and GUS promoter lines.Transcriptomic responses to PIPL3 peptide treatment suggested a role in regulation of biotic stress responses and cell wall modification.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.


Phylogenetic relationship between the IDA/IDL and PIP/PIPL peptides. (A) Sequence logo representation of the conserved C-terminal of IDA/IDL, PIP/PIPL and CEP peptides. (B) N-J and ML trees were constructed based on the protein alignment of the IDA/IDL and PIP/PIPL families shown in Fig. 1A. The N-J trees are shown. The overall topologies for the N-J and ML trees are the same. Bootstrap confidence values above 50% for N-J (first value) and ML (second value) are shown in the tree.
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Figure 2: Phylogenetic relationship between the IDA/IDL and PIP/PIPL peptides. (A) Sequence logo representation of the conserved C-terminal of IDA/IDL, PIP/PIPL and CEP peptides. (B) N-J and ML trees were constructed based on the protein alignment of the IDA/IDL and PIP/PIPL families shown in Fig. 1A. The N-J trees are shown. The overall topologies for the N-J and ML trees are the same. Bootstrap confidence values above 50% for N-J (first value) and ML (second value) are shown in the tree.

Mentions: Two studies of the CEP family of small peptides, consisting of 15 members, have recently been published (Delay et al., 2013; Roberts et al., 2013). Two of the CEP family members, CEP13 and CEP14, show similarity to the PIP/PIPLs. A sequence consensus logo of the SGPS motif and surrounding residues was made for the IDL and PIP/PIPL families as well as for the CEP family (Fig. 2A). The SGPS motif (position 4–7) is conserved in IDLs and PIP/PIPLs. A second conserved motif (GxGH, where x is any amino acid) was seen at the C-terminal end of all PIP/PIPLs. The SGPS motif was not found in the CEPs; however, the family was characterized by the C-terminal SPG(I/V)GH sequence, which resembles the C-terminal end of the PIP/PIPLs. Thus, the putative ligand domain of the PIP/PIPLs shares features with both IDLs and CEPs.


The IDA/IDA-LIKE and PIP/PIP-LIKE gene families in Arabidopsis: phylogenetic relationship, expression patterns, and transcriptional effect of the PIPL3 peptide.

Vie AK, Najafi J, Liu B, Winge P, Butenko MA, Hornslien KS, Kumpf R, Aalen RB, Bones AM, Brembu T - J. Exp. Bot. (2015)

Phylogenetic relationship between the IDA/IDL and PIP/PIPL peptides. (A) Sequence logo representation of the conserved C-terminal of IDA/IDL, PIP/PIPL and CEP peptides. (B) N-J and ML trees were constructed based on the protein alignment of the IDA/IDL and PIP/PIPL families shown in Fig. 1A. The N-J trees are shown. The overall topologies for the N-J and ML trees are the same. Bootstrap confidence values above 50% for N-J (first value) and ML (second value) are shown in the tree.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526919&req=5

Figure 2: Phylogenetic relationship between the IDA/IDL and PIP/PIPL peptides. (A) Sequence logo representation of the conserved C-terminal of IDA/IDL, PIP/PIPL and CEP peptides. (B) N-J and ML trees were constructed based on the protein alignment of the IDA/IDL and PIP/PIPL families shown in Fig. 1A. The N-J trees are shown. The overall topologies for the N-J and ML trees are the same. Bootstrap confidence values above 50% for N-J (first value) and ML (second value) are shown in the tree.
Mentions: Two studies of the CEP family of small peptides, consisting of 15 members, have recently been published (Delay et al., 2013; Roberts et al., 2013). Two of the CEP family members, CEP13 and CEP14, show similarity to the PIP/PIPLs. A sequence consensus logo of the SGPS motif and surrounding residues was made for the IDL and PIP/PIPL families as well as for the CEP family (Fig. 2A). The SGPS motif (position 4–7) is conserved in IDLs and PIP/PIPLs. A second conserved motif (GxGH, where x is any amino acid) was seen at the C-terminal end of all PIP/PIPLs. The SGPS motif was not found in the CEPs; however, the family was characterized by the C-terminal SPG(I/V)GH sequence, which resembles the C-terminal end of the PIP/PIPLs. Thus, the putative ligand domain of the PIP/PIPLs shares features with both IDLs and CEPs.

Bottom Line: Furthermore, we provide data that the recently identified PAMP-INDUCED SECRETED PEPTIDE (PIP) and PIP-LIKE (PIPL) peptides, which show similarity to the IDL and C-TERMINALLY ENCODED PEPTIDE (CEP) peptides, are not only involved in innate immune response in Arabidopsis but are also induced by abiotic stress.Expression patterns of the IDA/IDL and PIP/PIPL genes were analysed using in silico data, qRT-PCR and GUS promoter lines.Transcriptomic responses to PIPL3 peptide treatment suggested a role in regulation of biotic stress responses and cell wall modification.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.