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Mathematical modelling of WOX5- and CLE40-mediated columella stem cell homeostasis in Arabidopsis.

Richards S, Wink RH, Simon R - J. Exp. Bot. (2015)

Bottom Line: We have also found that WOX5 contributes to, but is not absolutely necessary for, CSC maintenance.Furthermore, our modelling led us to postulate an additional signalling molecule that promotes CSC maintenance.We propose that this WOX5-independent signal originates in the QC, is targeted by CLE40 signalling and is capable of maintaining CSCs.

View Article: PubMed Central - PubMed

Affiliation: Institute of Developmental Genetics, Heinrich Heine University, 40225 Düsseldorf, Germany.

No MeSH data available.


CSC maintenance in Col-0 and wox5-1, cle40-2 and wox5-1/cle40-2 mutants. (A) Wild-type (Col-0) roots typically maintain stem cells in the D1 layer. Roots lacking CLE40 accumulate more stem cells than wild type, as cle40-2 mutants more frequently lack starch in D2 as well as D1. Roots lacking WOX5 accumulate less stem cells than the wild type, with a higher frequency of starch in D1. Roots lacking both WOX5 and CLE40 partially rescue the wox5-1 mutant phenotype. Typical phenotypes are shown for (B) Col-0, and mutants (C) cle40-2, (D) wox5-1 and (E) wox5-1/cle40-2. White arrows indicate the QC position, pink indicates starch-free cell layers, and blue indicates the first layer with starch. Bars, 30 µm.
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Figure 7: CSC maintenance in Col-0 and wox5-1, cle40-2 and wox5-1/cle40-2 mutants. (A) Wild-type (Col-0) roots typically maintain stem cells in the D1 layer. Roots lacking CLE40 accumulate more stem cells than wild type, as cle40-2 mutants more frequently lack starch in D2 as well as D1. Roots lacking WOX5 accumulate less stem cells than the wild type, with a higher frequency of starch in D1. Roots lacking both WOX5 and CLE40 partially rescue the wox5-1 mutant phenotype. Typical phenotypes are shown for (B) Col-0, and mutants (C) cle40-2, (D) wox5-1 and (E) wox5-1/cle40-2. White arrows indicate the QC position, pink indicates starch-free cell layers, and blue indicates the first layer with starch. Bars, 30 µm.

Mentions: Surprisingly, a partial rescue of the wox5-1 phenotype was observed in the wox5-1/cle40-2 double mutants (Fig. 7). Since the C/W multi-cell model assumes that W, a WOX5-dependent signal, is necessary for CSC maintenance, it is impossible for the model to correctly predict this result; if there is no WOX5, there is no W, and according to the model there are no CSCs present. The failure of the model indicates that a basic assumption was wrong: WOX5 is not absolutely necessary for CSC maintenance.


Mathematical modelling of WOX5- and CLE40-mediated columella stem cell homeostasis in Arabidopsis.

Richards S, Wink RH, Simon R - J. Exp. Bot. (2015)

CSC maintenance in Col-0 and wox5-1, cle40-2 and wox5-1/cle40-2 mutants. (A) Wild-type (Col-0) roots typically maintain stem cells in the D1 layer. Roots lacking CLE40 accumulate more stem cells than wild type, as cle40-2 mutants more frequently lack starch in D2 as well as D1. Roots lacking WOX5 accumulate less stem cells than the wild type, with a higher frequency of starch in D1. Roots lacking both WOX5 and CLE40 partially rescue the wox5-1 mutant phenotype. Typical phenotypes are shown for (B) Col-0, and mutants (C) cle40-2, (D) wox5-1 and (E) wox5-1/cle40-2. White arrows indicate the QC position, pink indicates starch-free cell layers, and blue indicates the first layer with starch. Bars, 30 µm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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Figure 7: CSC maintenance in Col-0 and wox5-1, cle40-2 and wox5-1/cle40-2 mutants. (A) Wild-type (Col-0) roots typically maintain stem cells in the D1 layer. Roots lacking CLE40 accumulate more stem cells than wild type, as cle40-2 mutants more frequently lack starch in D2 as well as D1. Roots lacking WOX5 accumulate less stem cells than the wild type, with a higher frequency of starch in D1. Roots lacking both WOX5 and CLE40 partially rescue the wox5-1 mutant phenotype. Typical phenotypes are shown for (B) Col-0, and mutants (C) cle40-2, (D) wox5-1 and (E) wox5-1/cle40-2. White arrows indicate the QC position, pink indicates starch-free cell layers, and blue indicates the first layer with starch. Bars, 30 µm.
Mentions: Surprisingly, a partial rescue of the wox5-1 phenotype was observed in the wox5-1/cle40-2 double mutants (Fig. 7). Since the C/W multi-cell model assumes that W, a WOX5-dependent signal, is necessary for CSC maintenance, it is impossible for the model to correctly predict this result; if there is no WOX5, there is no W, and according to the model there are no CSCs present. The failure of the model indicates that a basic assumption was wrong: WOX5 is not absolutely necessary for CSC maintenance.

Bottom Line: We have also found that WOX5 contributes to, but is not absolutely necessary for, CSC maintenance.Furthermore, our modelling led us to postulate an additional signalling molecule that promotes CSC maintenance.We propose that this WOX5-independent signal originates in the QC, is targeted by CLE40 signalling and is capable of maintaining CSCs.

View Article: PubMed Central - PubMed

Affiliation: Institute of Developmental Genetics, Heinrich Heine University, 40225 Düsseldorf, Germany.

No MeSH data available.