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The Arabidopsis Pep-PEPR system is induced by herbivore feeding and contributes to JA-mediated plant defence against herbivory.

Klauser D, Desurmont GA, Glauser G, Vallat A, Flury P, Boller T, Turlings TC, Bartels S - J. Exp. Bot. (2015)

Bottom Line: By using Arabidopsis GUS-reporter lines, it is now shown that the promoters of both Pep-receptors, PEPR1 and PEPR2, as well as PROPEP3 are strongly activated upon herbivore attack.Moreover, pepr1 pepr2 double mutant plants, which are insensitive to Peps, display a reduced resistance to feeding Spodoptera littoralis larvae and a reduced accumulation of jasmonic acid upon exposure to herbivore oral secretions.Taken together, these lines of evidence extend the role of the AtPep-PEPR system as a danger detection mechanism from microbial pathogens to herbivores and further underline its strong interaction with jasmonic acid signalling.

View Article: PubMed Central - PubMed

Affiliation: Zürich-Basel Plant Science Center, University of Basel, Department of Environmental Sciences, Botany, Hebelstrasse 1, CH-4056 Basel, Switzerland.

No MeSH data available.


Related in: MedlinePlus

Spodoptera feeding strongly induces the promoters of PEPR1, PEPR2, and PROPEP3. Leaves of transgenic Arabidopsis plants expressing pPEPR::GUS and pPROPEP3::GUS reporter constructs were either wounded using cork borers or exposed to feeding Spodoptera littoralis (S.l.) larvae. After 12h, the leaves were detached from the plant, fixed, and stained. For each construct, two independent lines were assessed with very similar results.
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Figure 2: Spodoptera feeding strongly induces the promoters of PEPR1, PEPR2, and PROPEP3. Leaves of transgenic Arabidopsis plants expressing pPEPR::GUS and pPROPEP3::GUS reporter constructs were either wounded using cork borers or exposed to feeding Spodoptera littoralis (S.l.) larvae. After 12h, the leaves were detached from the plant, fixed, and stained. For each construct, two independent lines were assessed with very similar results.

Mentions: To assess directly PROPEP and PEPR gene expression upon herbivore attack, the response of PROPEP3 and both PEPRs to feeding S. littoralis larvae were analysed. Similar to the OS application, feeding of S. littoralis also strongly activated all three promoters (Fig. 2). The increased activity of the PEPR promoters is located directly around areas of herbivore attack and does not extend to unharmed parts of the leaves. In the case of the promoter of PROPEP3 the detected GUS staining was not limited to the actual feeding sites, but also spread into the leaf veins (Fig. 2). No GUS signal was detected upon wounding the plants by cutting out small leaf pieces using a sterile cork borer (Fig. 2).


The Arabidopsis Pep-PEPR system is induced by herbivore feeding and contributes to JA-mediated plant defence against herbivory.

Klauser D, Desurmont GA, Glauser G, Vallat A, Flury P, Boller T, Turlings TC, Bartels S - J. Exp. Bot. (2015)

Spodoptera feeding strongly induces the promoters of PEPR1, PEPR2, and PROPEP3. Leaves of transgenic Arabidopsis plants expressing pPEPR::GUS and pPROPEP3::GUS reporter constructs were either wounded using cork borers or exposed to feeding Spodoptera littoralis (S.l.) larvae. After 12h, the leaves were detached from the plant, fixed, and stained. For each construct, two independent lines were assessed with very similar results.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526914&req=5

Figure 2: Spodoptera feeding strongly induces the promoters of PEPR1, PEPR2, and PROPEP3. Leaves of transgenic Arabidopsis plants expressing pPEPR::GUS and pPROPEP3::GUS reporter constructs were either wounded using cork borers or exposed to feeding Spodoptera littoralis (S.l.) larvae. After 12h, the leaves were detached from the plant, fixed, and stained. For each construct, two independent lines were assessed with very similar results.
Mentions: To assess directly PROPEP and PEPR gene expression upon herbivore attack, the response of PROPEP3 and both PEPRs to feeding S. littoralis larvae were analysed. Similar to the OS application, feeding of S. littoralis also strongly activated all three promoters (Fig. 2). The increased activity of the PEPR promoters is located directly around areas of herbivore attack and does not extend to unharmed parts of the leaves. In the case of the promoter of PROPEP3 the detected GUS staining was not limited to the actual feeding sites, but also spread into the leaf veins (Fig. 2). No GUS signal was detected upon wounding the plants by cutting out small leaf pieces using a sterile cork borer (Fig. 2).

Bottom Line: By using Arabidopsis GUS-reporter lines, it is now shown that the promoters of both Pep-receptors, PEPR1 and PEPR2, as well as PROPEP3 are strongly activated upon herbivore attack.Moreover, pepr1 pepr2 double mutant plants, which are insensitive to Peps, display a reduced resistance to feeding Spodoptera littoralis larvae and a reduced accumulation of jasmonic acid upon exposure to herbivore oral secretions.Taken together, these lines of evidence extend the role of the AtPep-PEPR system as a danger detection mechanism from microbial pathogens to herbivores and further underline its strong interaction with jasmonic acid signalling.

View Article: PubMed Central - PubMed

Affiliation: Zürich-Basel Plant Science Center, University of Basel, Department of Environmental Sciences, Botany, Hebelstrasse 1, CH-4056 Basel, Switzerland.

No MeSH data available.


Related in: MedlinePlus