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Novel MtCEP1 peptides produced in vivo differentially regulate root development in Medicago truncatula.

Mohd-Radzman NA, Binos S, Truong TT, Imin N, Mariani M, Djordjevic MA - J. Exp. Bot. (2015)

Bottom Line: In contrast, the domain 2 peptide hydroxylated at Pro11 (D2:HyP11) increased stage III-IV lateral root primordium numbers by 6-fold (P < 0.001) which failed to emerge.Auxin addition at levels which stimulated lateral root formation in wild-type plants had little or no ameliorating effect on CEP peptide-mediated inhibition of lateral root formation or emergence.The results showed that CEP primary sequence and post-translational modifications influence peptide activities and the improved isolation procedure effectively and reproducibly identifies and characterises CEPs.

View Article: PubMed Central - PubMed

Affiliation: Division of Plant Sciences, Research School of Biology, College of Medicine, Biology and Environment, The Australian National University, Canberra ACT 0200, Australia.

No MeSH data available.


Related in: MedlinePlus

Interactions of MtCEP1 peptides with NAA treatment and CEP peptide effect on GH3:GUS expression in roots. (A) Lateral root formation on 14-day-old plants grown on medium with or without synthetic D1:HyP4,11 or D2:HyP11 in the presence of NAA at 10–9 M or 10-10 M or 0M as the control. (Student’s t-test; **, P ≤ 0.01; n ≥ 16). (B) Non-treated control roots showing the formation of young lateral root primordium expressing GH3:GUS (white asterisk). (C) The D2:HyP11 peptide which stimulated the formation of lateral root primordia showed strong staining (white asterisks). (D, E) CCP sites (red asterisks) induced by the D2:HyP11 (D) and D1:HyP4,11 (E) are shown. Scale bars, 100 µm.
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Figure 5: Interactions of MtCEP1 peptides with NAA treatment and CEP peptide effect on GH3:GUS expression in roots. (A) Lateral root formation on 14-day-old plants grown on medium with or without synthetic D1:HyP4,11 or D2:HyP11 in the presence of NAA at 10–9 M or 10-10 M or 0M as the control. (Student’s t-test; **, P ≤ 0.01; n ≥ 16). (B) Non-treated control roots showing the formation of young lateral root primordium expressing GH3:GUS (white asterisk). (C) The D2:HyP11 peptide which stimulated the formation of lateral root primordia showed strong staining (white asterisks). (D, E) CCP sites (red asterisks) induced by the D2:HyP11 (D) and D1:HyP4,11 (E) are shown. Scale bars, 100 µm.

Mentions: NAA stimulates lateral root initiation by specifying lateral root founder cells (Casimiro et al., 2001; De Smet et al., 2007; Dubrovsky et al., 2008) and promotes lateral root emergence or both initiation and emergence in Arabidopsis, rice and tobacco (Bhalerao et al., 2002; Campanoni and Nick, 2005; Sreevidya et al., 2010). Therefore, NAA was assessed for its ability to override the inhibitory effects on lateral root formation of these two peptides (Fig. 5A). Using a titration assay, we determined that the optimal NAA concentration for stimulating lateral root emergence in Medicago without inhibiting the primary root growth was 10-10 M (Supplementary Fig. S6). Therefore, the effects of adding NAA at 10-10 or 10–9 M were assessed. NAA at 10-10 M significantly increased the number of emerged lateral roots in control samples. NAA (10-10 M) in the presence of D1:HyP4,11 induced a small but significant increase in the number of emerged lateral roots compared to roots treated only with the peptide, however the overall level was over three-fold lower than 10-10 M NAA treatment alone. Further raising NAA to 10–9 M did not significant increase lateral root number. NAA addition at 10-10 M or 10–9 M did not increase the number of emerged lateral roots on plant grown with D2:HyP11.


Novel MtCEP1 peptides produced in vivo differentially regulate root development in Medicago truncatula.

Mohd-Radzman NA, Binos S, Truong TT, Imin N, Mariani M, Djordjevic MA - J. Exp. Bot. (2015)

Interactions of MtCEP1 peptides with NAA treatment and CEP peptide effect on GH3:GUS expression in roots. (A) Lateral root formation on 14-day-old plants grown on medium with or without synthetic D1:HyP4,11 or D2:HyP11 in the presence of NAA at 10–9 M or 10-10 M or 0M as the control. (Student’s t-test; **, P ≤ 0.01; n ≥ 16). (B) Non-treated control roots showing the formation of young lateral root primordium expressing GH3:GUS (white asterisk). (C) The D2:HyP11 peptide which stimulated the formation of lateral root primordia showed strong staining (white asterisks). (D, E) CCP sites (red asterisks) induced by the D2:HyP11 (D) and D1:HyP4,11 (E) are shown. Scale bars, 100 µm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 5: Interactions of MtCEP1 peptides with NAA treatment and CEP peptide effect on GH3:GUS expression in roots. (A) Lateral root formation on 14-day-old plants grown on medium with or without synthetic D1:HyP4,11 or D2:HyP11 in the presence of NAA at 10–9 M or 10-10 M or 0M as the control. (Student’s t-test; **, P ≤ 0.01; n ≥ 16). (B) Non-treated control roots showing the formation of young lateral root primordium expressing GH3:GUS (white asterisk). (C) The D2:HyP11 peptide which stimulated the formation of lateral root primordia showed strong staining (white asterisks). (D, E) CCP sites (red asterisks) induced by the D2:HyP11 (D) and D1:HyP4,11 (E) are shown. Scale bars, 100 µm.
Mentions: NAA stimulates lateral root initiation by specifying lateral root founder cells (Casimiro et al., 2001; De Smet et al., 2007; Dubrovsky et al., 2008) and promotes lateral root emergence or both initiation and emergence in Arabidopsis, rice and tobacco (Bhalerao et al., 2002; Campanoni and Nick, 2005; Sreevidya et al., 2010). Therefore, NAA was assessed for its ability to override the inhibitory effects on lateral root formation of these two peptides (Fig. 5A). Using a titration assay, we determined that the optimal NAA concentration for stimulating lateral root emergence in Medicago without inhibiting the primary root growth was 10-10 M (Supplementary Fig. S6). Therefore, the effects of adding NAA at 10-10 or 10–9 M were assessed. NAA at 10-10 M significantly increased the number of emerged lateral roots in control samples. NAA (10-10 M) in the presence of D1:HyP4,11 induced a small but significant increase in the number of emerged lateral roots compared to roots treated only with the peptide, however the overall level was over three-fold lower than 10-10 M NAA treatment alone. Further raising NAA to 10–9 M did not significant increase lateral root number. NAA addition at 10-10 M or 10–9 M did not increase the number of emerged lateral roots on plant grown with D2:HyP11.

Bottom Line: In contrast, the domain 2 peptide hydroxylated at Pro11 (D2:HyP11) increased stage III-IV lateral root primordium numbers by 6-fold (P < 0.001) which failed to emerge.Auxin addition at levels which stimulated lateral root formation in wild-type plants had little or no ameliorating effect on CEP peptide-mediated inhibition of lateral root formation or emergence.The results showed that CEP primary sequence and post-translational modifications influence peptide activities and the improved isolation procedure effectively and reproducibly identifies and characterises CEPs.

View Article: PubMed Central - PubMed

Affiliation: Division of Plant Sciences, Research School of Biology, College of Medicine, Biology and Environment, The Australian National University, Canberra ACT 0200, Australia.

No MeSH data available.


Related in: MedlinePlus