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Antibacterial and residual antimicrobial activities against Enterococcus faecalis biofilm: A comparison between EDTA, chlorhexidine, cetrimide, MTAD and QMix.

Zhang R, Chen M, Lu Y, Guo X, Qiao F, Wu L - Sci Rep (2015)

Bottom Line: The number of viable E. faecalis was significantly fewer in the irrigant-treated groups compared with the untreated control (P < 0.05).Residual antimicrobial activities of CHX were significantly higher at 12 h, 24 h and 36 h compared to untreated control (P < 0.05).All five root canal irrigants were effective to some extent against E. faecalis, but QMix and CHX had the strongest, and CHX the longest (up to 36 h), antimicrobial activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Endodontics and Restorative Dentistry, School of Stomatology, Tianjin Medical University, Tianjin, China.

ABSTRACT
We compared the antibacterial and residual antimicrobial activities of five root canal irrigants (17% EDTA,2% chlorhexidine,0.2% cetrimide, MTAD, and QMix) in a model of Enterococcus faecalis biofilm formation. Sixty dentin blocks with 3-week E. faecalis biofilm were divided into six equal groups and flushed with irrigant for 2 min or left untreated. A blank control group was also established. Antibacterial activities of the irrigants were evaluated by counting colony forming units. To test residual antimicrobial activities, 280 dentin blocks were divided into seven equal groups and flushed with irrigant for 2 min or left untreated and then incubated with E. faecalis suspension for 48 h, or used as a blank. No bacteria were observed in the blank control group. The number of viable E. faecalis was significantly fewer in the irrigant-treated groups compared with the untreated control (P < 0.05). Among the five irrigants, QMix had the strongest antibacterial activity. Residual antimicrobial activities of CHX were significantly higher at 12 h, 24 h and 36 h compared to untreated control (P < 0.05). All five root canal irrigants were effective to some extent against E. faecalis, but QMix and CHX had the strongest, and CHX the longest (up to 36 h), antimicrobial activity.

No MeSH data available.


Related in: MedlinePlus

Residual antimicrobial activities (lgCFUs) of the five root canal irrigants (EDTA, CHX, CTR, MTAD, and QMix) and untreated control groups at 12ā€‰h, 24ā€‰h, 36ā€‰h, and 48ā€‰h.
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f2: Residual antimicrobial activities (lgCFUs) of the five root canal irrigants (EDTA, CHX, CTR, MTAD, and QMix) and untreated control groups at 12ā€‰h, 24ā€‰h, 36ā€‰h, and 48ā€‰h.

Mentions: Differences in the residual antimicrobial activities among the six groups at 12ā€‰h, 24ā€‰h, 36ā€‰h, and 48ā€‰h are shown in Table 1 and Fig. 2. Compared with the untreated control group, the residual antimicrobial activities of the EDTA, CHX, and MTAD groups were not significantly different (Pā€‰>ā€‰0.05). On the other hand, the lgCFUs of the CTR and QMix groups were significantly lower than those of the untreated control group (āˆ’3.85ā€‰Ā±ā€‰2.89 [CTR] and āˆ’6.00ā€‰Ā±ā€‰0.00 [QMix] vs. 1.89ā€‰Ā±ā€‰0.14 [untreated control]; Pā€‰<ā€‰0.05) at 12ā€‰h. There was no significant difference in the residual antimicrobial activities among the EDTA, MTAD, QMix, and the untreated control groups at 24ā€‰h (Pā€‰>ā€‰0.05). However, the lgCFUs of the CHX and CTR groups were significantly lower than those of the untreated control group (0.86ā€‰Ā±ā€‰6.51 and āˆ’2.33ā€‰Ā±ā€‰5.50 vs. 8.62ā€‰Ā±ā€‰0.02; Pā€‰<ā€‰0.05) at 24ā€‰h. At 36ā€‰h, only the lgCFUs of the CHX group was significantly lower than those of the untreated control group (0.31ā€‰Ā±ā€‰4.72 vs. 8.15ā€‰Ā±ā€‰0.09; Pā€‰<ā€‰0.05). There was no significant difference in the residual antimicrobial activities among the treated and untreated control groups at 48ā€‰h (Pā€‰>ā€‰0.05).


Antibacterial and residual antimicrobial activities against Enterococcus faecalis biofilm: A comparison between EDTA, chlorhexidine, cetrimide, MTAD and QMix.

Zhang R, Chen M, Lu Y, Guo X, Qiao F, Wu L - Sci Rep (2015)

Residual antimicrobial activities (lgCFUs) of the five root canal irrigants (EDTA, CHX, CTR, MTAD, and QMix) and untreated control groups at 12ā€‰h, 24ā€‰h, 36ā€‰h, and 48ā€‰h.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526883&req=5

f2: Residual antimicrobial activities (lgCFUs) of the five root canal irrigants (EDTA, CHX, CTR, MTAD, and QMix) and untreated control groups at 12ā€‰h, 24ā€‰h, 36ā€‰h, and 48ā€‰h.
Mentions: Differences in the residual antimicrobial activities among the six groups at 12ā€‰h, 24ā€‰h, 36ā€‰h, and 48ā€‰h are shown in Table 1 and Fig. 2. Compared with the untreated control group, the residual antimicrobial activities of the EDTA, CHX, and MTAD groups were not significantly different (Pā€‰>ā€‰0.05). On the other hand, the lgCFUs of the CTR and QMix groups were significantly lower than those of the untreated control group (āˆ’3.85ā€‰Ā±ā€‰2.89 [CTR] and āˆ’6.00ā€‰Ā±ā€‰0.00 [QMix] vs. 1.89ā€‰Ā±ā€‰0.14 [untreated control]; Pā€‰<ā€‰0.05) at 12ā€‰h. There was no significant difference in the residual antimicrobial activities among the EDTA, MTAD, QMix, and the untreated control groups at 24ā€‰h (Pā€‰>ā€‰0.05). However, the lgCFUs of the CHX and CTR groups were significantly lower than those of the untreated control group (0.86ā€‰Ā±ā€‰6.51 and āˆ’2.33ā€‰Ā±ā€‰5.50 vs. 8.62ā€‰Ā±ā€‰0.02; Pā€‰<ā€‰0.05) at 24ā€‰h. At 36ā€‰h, only the lgCFUs of the CHX group was significantly lower than those of the untreated control group (0.31ā€‰Ā±ā€‰4.72 vs. 8.15ā€‰Ā±ā€‰0.09; Pā€‰<ā€‰0.05). There was no significant difference in the residual antimicrobial activities among the treated and untreated control groups at 48ā€‰h (Pā€‰>ā€‰0.05).

Bottom Line: The number of viable E. faecalis was significantly fewer in the irrigant-treated groups compared with the untreated control (P < 0.05).Residual antimicrobial activities of CHX were significantly higher at 12 h, 24 h and 36 h compared to untreated control (P < 0.05).All five root canal irrigants were effective to some extent against E. faecalis, but QMix and CHX had the strongest, and CHX the longest (up to 36 h), antimicrobial activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Endodontics and Restorative Dentistry, School of Stomatology, Tianjin Medical University, Tianjin, China.

ABSTRACT
We compared the antibacterial and residual antimicrobial activities of five root canal irrigants (17% EDTA,2% chlorhexidine,0.2% cetrimide, MTAD, and QMix) in a model of Enterococcus faecalis biofilm formation. Sixty dentin blocks with 3-week E. faecalis biofilm were divided into six equal groups and flushed with irrigant for 2 min or left untreated. A blank control group was also established. Antibacterial activities of the irrigants were evaluated by counting colony forming units. To test residual antimicrobial activities, 280 dentin blocks were divided into seven equal groups and flushed with irrigant for 2 min or left untreated and then incubated with E. faecalis suspension for 48 h, or used as a blank. No bacteria were observed in the blank control group. The number of viable E. faecalis was significantly fewer in the irrigant-treated groups compared with the untreated control (P < 0.05). Among the five irrigants, QMix had the strongest antibacterial activity. Residual antimicrobial activities of CHX were significantly higher at 12 h, 24 h and 36 h compared to untreated control (P < 0.05). All five root canal irrigants were effective to some extent against E. faecalis, but QMix and CHX had the strongest, and CHX the longest (up to 36 h), antimicrobial activity.

No MeSH data available.


Related in: MedlinePlus