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Antibacterial and residual antimicrobial activities against Enterococcus faecalis biofilm: A comparison between EDTA, chlorhexidine, cetrimide, MTAD and QMix.

Zhang R, Chen M, Lu Y, Guo X, Qiao F, Wu L - Sci Rep (2015)

Bottom Line: The number of viable E. faecalis was significantly fewer in the irrigant-treated groups compared with the untreated control (P < 0.05).Residual antimicrobial activities of CHX were significantly higher at 12 h, 24 h and 36 h compared to untreated control (P < 0.05).All five root canal irrigants were effective to some extent against E. faecalis, but QMix and CHX had the strongest, and CHX the longest (up to 36 h), antimicrobial activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Endodontics and Restorative Dentistry, School of Stomatology, Tianjin Medical University, Tianjin, China.

ABSTRACT
We compared the antibacterial and residual antimicrobial activities of five root canal irrigants (17% EDTA,2% chlorhexidine,0.2% cetrimide, MTAD, and QMix) in a model of Enterococcus faecalis biofilm formation. Sixty dentin blocks with 3-week E. faecalis biofilm were divided into six equal groups and flushed with irrigant for 2 min or left untreated. A blank control group was also established. Antibacterial activities of the irrigants were evaluated by counting colony forming units. To test residual antimicrobial activities, 280 dentin blocks were divided into seven equal groups and flushed with irrigant for 2 min or left untreated and then incubated with E. faecalis suspension for 48 h, or used as a blank. No bacteria were observed in the blank control group. The number of viable E. faecalis was significantly fewer in the irrigant-treated groups compared with the untreated control (P < 0.05). Among the five irrigants, QMix had the strongest antibacterial activity. Residual antimicrobial activities of CHX were significantly higher at 12 h, 24 h and 36 h compared to untreated control (P < 0.05). All five root canal irrigants were effective to some extent against E. faecalis, but QMix and CHX had the strongest, and CHX the longest (up to 36 h), antimicrobial activity.

No MeSH data available.


Related in: MedlinePlus

Antibacterial activities (lgCFUs) of the five root canal irrigants (EDTA, CHX, CTR, MTAD, and QMix) and untreated control groups.*P < 0.05, **P < 0.01 compared with the untreated control group.
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f1: Antibacterial activities (lgCFUs) of the five root canal irrigants (EDTA, CHX, CTR, MTAD, and QMix) and untreated control groups.*P < 0.05, **P < 0.01 compared with the untreated control group.

Mentions: No bacteria were observed in the blank control group. Compared with the untreated control group, E. faecalis IgCFUs in dentin blocks treated with EDTA, CHX, CTR, MTAD, and QMix were significantly reduced (P < 0.05) (Table 1, Fig. 1). The QMix group had the lowest lgCFU value (2.31 ± 0.32), followed by the CHX group (2.41 ± 0.21), the MTAD group (3.26 ± 0.23), the CTR group (3.71 ± 0.72), and the EDTA group (4.37 ± 0.31). The antibacterial activities of the six groups were significantly different (P < 0.05), except between CHX and QMix groups (P > 0.05).


Antibacterial and residual antimicrobial activities against Enterococcus faecalis biofilm: A comparison between EDTA, chlorhexidine, cetrimide, MTAD and QMix.

Zhang R, Chen M, Lu Y, Guo X, Qiao F, Wu L - Sci Rep (2015)

Antibacterial activities (lgCFUs) of the five root canal irrigants (EDTA, CHX, CTR, MTAD, and QMix) and untreated control groups.*P < 0.05, **P < 0.01 compared with the untreated control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526883&req=5

f1: Antibacterial activities (lgCFUs) of the five root canal irrigants (EDTA, CHX, CTR, MTAD, and QMix) and untreated control groups.*P < 0.05, **P < 0.01 compared with the untreated control group.
Mentions: No bacteria were observed in the blank control group. Compared with the untreated control group, E. faecalis IgCFUs in dentin blocks treated with EDTA, CHX, CTR, MTAD, and QMix were significantly reduced (P < 0.05) (Table 1, Fig. 1). The QMix group had the lowest lgCFU value (2.31 ± 0.32), followed by the CHX group (2.41 ± 0.21), the MTAD group (3.26 ± 0.23), the CTR group (3.71 ± 0.72), and the EDTA group (4.37 ± 0.31). The antibacterial activities of the six groups were significantly different (P < 0.05), except between CHX and QMix groups (P > 0.05).

Bottom Line: The number of viable E. faecalis was significantly fewer in the irrigant-treated groups compared with the untreated control (P < 0.05).Residual antimicrobial activities of CHX were significantly higher at 12 h, 24 h and 36 h compared to untreated control (P < 0.05).All five root canal irrigants were effective to some extent against E. faecalis, but QMix and CHX had the strongest, and CHX the longest (up to 36 h), antimicrobial activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Endodontics and Restorative Dentistry, School of Stomatology, Tianjin Medical University, Tianjin, China.

ABSTRACT
We compared the antibacterial and residual antimicrobial activities of five root canal irrigants (17% EDTA,2% chlorhexidine,0.2% cetrimide, MTAD, and QMix) in a model of Enterococcus faecalis biofilm formation. Sixty dentin blocks with 3-week E. faecalis biofilm were divided into six equal groups and flushed with irrigant for 2 min or left untreated. A blank control group was also established. Antibacterial activities of the irrigants were evaluated by counting colony forming units. To test residual antimicrobial activities, 280 dentin blocks were divided into seven equal groups and flushed with irrigant for 2 min or left untreated and then incubated with E. faecalis suspension for 48 h, or used as a blank. No bacteria were observed in the blank control group. The number of viable E. faecalis was significantly fewer in the irrigant-treated groups compared with the untreated control (P < 0.05). Among the five irrigants, QMix had the strongest antibacterial activity. Residual antimicrobial activities of CHX were significantly higher at 12 h, 24 h and 36 h compared to untreated control (P < 0.05). All five root canal irrigants were effective to some extent against E. faecalis, but QMix and CHX had the strongest, and CHX the longest (up to 36 h), antimicrobial activity.

No MeSH data available.


Related in: MedlinePlus