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Somatostatin receptor 1-5; expression profiles during rat development.

Ludvigsen E, Carlsson C, Tiensuu Janson E, Sandler S, Stridsberg M - Ups. J. Med. Sci. (2015)

Bottom Line: In mRNA isolated from whole rat embryos SSTR1-2 and SSTR4 expression showed a peak at day 14, while SSTR3 mRNA was not present until day 15.The present data suggest a role for SSTRs during the development of the rat embryo.Subsequent functional studies may elucidate regulatory roles of specific SSTRs for the growth and differentiation of the pancreas as well as other organs.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Cell Biology, Uppsala University , Uppsala , Sweden.

ABSTRACT

Background: Somatostatin acts through five receptor subtypes (SSTRs 1-5). We aimed to investigate SSTRs mRNA expression and protein distribution in whole rat embryos, with special emphasis on the pancreas.

Material and methods: Rat embryos were collected on embryonal days 10, 11, 12, 14, 15, 17, 19, 21, and at birth. Presence of SSTRs was investigated with RT-PCR techniques and immunohistochemistry.

Results: There was no SSTR5 mRNA expression in the whole rat embryos. All SSTR1-5 proteins were observed at embryonal day 10, but the localization varied between the different subtypes. From day 11 to birth SSTRs protein presence increased with time in major structures such as skin and cartilage. It remained similar over time in the heart and liver. In the fetal pancreas mRNA expression of SSTR2 and 4 was detected at day 14, and there was an increase up to birth. Only SSTR1 protein co-localized to a higher extent with the islet hormones studied. SSTR2 was present in all islet endocrine cells except for β-cells. In contrast, the immunostaining for SSTR3-4 was co-localized with insulin and PP, and, finally, SSTR5 with glucagon and pancreatic polypeptide. In mRNA isolated from whole rat embryos SSTR1-2 and SSTR4 expression showed a peak at day 14, while SSTR3 mRNA was not present until day 15.

Conclusion: The present data suggest a role for SSTRs during the development of the rat embryo. Subsequent functional studies may elucidate regulatory roles of specific SSTRs for the growth and differentiation of the pancreas as well as other organs.

No MeSH data available.


Expression of SSTR mRNAs in the embryonic rat pancreas amplified using real-time PCR. A: SSTR1; B: SSTR2; C: SSTR3; D: SSTR4; E: SSTR5. Data are given as mean relative expression (2-Δ(cpssts-cpTBP)) of embryonal pancreases from the same day.
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Figure 2: Expression of SSTR mRNAs in the embryonic rat pancreas amplified using real-time PCR. A: SSTR1; B: SSTR2; C: SSTR3; D: SSTR4; E: SSTR5. Data are given as mean relative expression (2-Δ(cpssts-cpTBP)) of embryonal pancreases from the same day.

Mentions: SSTR1: The expression of SSTR1 was faint at day 14. At day 15 the levels were higher and peaked at day 17. Subsequently, at day 19 it disappeared, but returned with high expression in the newborn. In the adult pancreas, expression of SSTR1 was very weak (Figure 2A).


Somatostatin receptor 1-5; expression profiles during rat development.

Ludvigsen E, Carlsson C, Tiensuu Janson E, Sandler S, Stridsberg M - Ups. J. Med. Sci. (2015)

Expression of SSTR mRNAs in the embryonic rat pancreas amplified using real-time PCR. A: SSTR1; B: SSTR2; C: SSTR3; D: SSTR4; E: SSTR5. Data are given as mean relative expression (2-Δ(cpssts-cpTBP)) of embryonal pancreases from the same day.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526871&req=5

Figure 2: Expression of SSTR mRNAs in the embryonic rat pancreas amplified using real-time PCR. A: SSTR1; B: SSTR2; C: SSTR3; D: SSTR4; E: SSTR5. Data are given as mean relative expression (2-Δ(cpssts-cpTBP)) of embryonal pancreases from the same day.
Mentions: SSTR1: The expression of SSTR1 was faint at day 14. At day 15 the levels were higher and peaked at day 17. Subsequently, at day 19 it disappeared, but returned with high expression in the newborn. In the adult pancreas, expression of SSTR1 was very weak (Figure 2A).

Bottom Line: In mRNA isolated from whole rat embryos SSTR1-2 and SSTR4 expression showed a peak at day 14, while SSTR3 mRNA was not present until day 15.The present data suggest a role for SSTRs during the development of the rat embryo.Subsequent functional studies may elucidate regulatory roles of specific SSTRs for the growth and differentiation of the pancreas as well as other organs.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Cell Biology, Uppsala University , Uppsala , Sweden.

ABSTRACT

Background: Somatostatin acts through five receptor subtypes (SSTRs 1-5). We aimed to investigate SSTRs mRNA expression and protein distribution in whole rat embryos, with special emphasis on the pancreas.

Material and methods: Rat embryos were collected on embryonal days 10, 11, 12, 14, 15, 17, 19, 21, and at birth. Presence of SSTRs was investigated with RT-PCR techniques and immunohistochemistry.

Results: There was no SSTR5 mRNA expression in the whole rat embryos. All SSTR1-5 proteins were observed at embryonal day 10, but the localization varied between the different subtypes. From day 11 to birth SSTRs protein presence increased with time in major structures such as skin and cartilage. It remained similar over time in the heart and liver. In the fetal pancreas mRNA expression of SSTR2 and 4 was detected at day 14, and there was an increase up to birth. Only SSTR1 protein co-localized to a higher extent with the islet hormones studied. SSTR2 was present in all islet endocrine cells except for β-cells. In contrast, the immunostaining for SSTR3-4 was co-localized with insulin and PP, and, finally, SSTR5 with glucagon and pancreatic polypeptide. In mRNA isolated from whole rat embryos SSTR1-2 and SSTR4 expression showed a peak at day 14, while SSTR3 mRNA was not present until day 15.

Conclusion: The present data suggest a role for SSTRs during the development of the rat embryo. Subsequent functional studies may elucidate regulatory roles of specific SSTRs for the growth and differentiation of the pancreas as well as other organs.

No MeSH data available.