Limits...
Diacetin, a reliable cue and private communication channel in a specialized pollination system.

Schäffler I, Steiner KE, Haid M, van Berkel SS, Gerlach G, Johnson SD, Wessjohann L, Dötterl S - Sci Rep (2015)

Bottom Line: The structural and obvious biosynthetic similarity between diacetin and associated floral oils make it a reliable cue for oil-collecting bees.It is easily perceived by oil bees, but can't be detected by other potential pollinators.Therefore, diacetin represents the first demonstrated private communication channel in a pollination system.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Ecology and Evolution, University of Salzburg, Hellbrunnerstr. 34, 5020 Salzburg, Austria [2] Department of Plant Systematics, University of Bayreuth, 95440 Bayreuth.

ABSTRACT
The interaction between floral oil secreting plants and oil-collecting bees is one of the most specialized of all pollination mutualisms. Yet, the specific stimuli used by the bees to locate their host flowers have remained elusive. This study identifies diacetin, a volatile acetylated glycerol, as a floral signal compound shared by unrelated oil plants from around the globe. Electrophysiological measurements of antennae and behavioural assays identified diacetin as the key volatile used by oil-collecting bees to locate their host flowers. Furthermore, electrophysiological measurements indicate that only oil-collecting bees are capable of detecting diacetin. The structural and obvious biosynthetic similarity between diacetin and associated floral oils make it a reliable cue for oil-collecting bees. It is easily perceived by oil bees, but can't be detected by other potential pollinators. Therefore, diacetin represents the first demonstrated private communication channel in a pollination system.

No MeSH data available.


Physiological dose-response curves of different bees for diacetin.Electroantennographic responses (EAG) of oil bees (Macropis fulvipes/Rediviva neliana) and non-oil bees (Melitta haemorrhoidalis/Apis mellifera) to different dilutions of diacetin. SEM = standard error of the mean. Means (within the same species) that share same letters or do not have letters are not significantly different.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4526864&req=5

f2: Physiological dose-response curves of different bees for diacetin.Electroantennographic responses (EAG) of oil bees (Macropis fulvipes/Rediviva neliana) and non-oil bees (Melitta haemorrhoidalis/Apis mellifera) to different dilutions of diacetin. SEM = standard error of the mean. Means (within the same species) that share same letters or do not have letters are not significantly different.

Mentions: Overall analysis revealed significant effects of bee species (F2,17 = 15.97, P < 0.001), dilution (F3,51 = 60.21, P < 0.001), and the bee species × dilution interaction (F6,51 = 17.94, P < 0.001) on antennal responses to diacetin. Antennal responses increased with increasing concentration of diacetin for M. fulvipes and Rediviva neliana oil bees, but not for honey bees (Fig. 2). As was the case for honey bees, there was no dilution effect of diacetin for the non-oil bee Melitta haemorrhoidalis (t = 1.00, df = 4, P = 0.37).


Diacetin, a reliable cue and private communication channel in a specialized pollination system.

Schäffler I, Steiner KE, Haid M, van Berkel SS, Gerlach G, Johnson SD, Wessjohann L, Dötterl S - Sci Rep (2015)

Physiological dose-response curves of different bees for diacetin.Electroantennographic responses (EAG) of oil bees (Macropis fulvipes/Rediviva neliana) and non-oil bees (Melitta haemorrhoidalis/Apis mellifera) to different dilutions of diacetin. SEM = standard error of the mean. Means (within the same species) that share same letters or do not have letters are not significantly different.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526864&req=5

f2: Physiological dose-response curves of different bees for diacetin.Electroantennographic responses (EAG) of oil bees (Macropis fulvipes/Rediviva neliana) and non-oil bees (Melitta haemorrhoidalis/Apis mellifera) to different dilutions of diacetin. SEM = standard error of the mean. Means (within the same species) that share same letters or do not have letters are not significantly different.
Mentions: Overall analysis revealed significant effects of bee species (F2,17 = 15.97, P < 0.001), dilution (F3,51 = 60.21, P < 0.001), and the bee species × dilution interaction (F6,51 = 17.94, P < 0.001) on antennal responses to diacetin. Antennal responses increased with increasing concentration of diacetin for M. fulvipes and Rediviva neliana oil bees, but not for honey bees (Fig. 2). As was the case for honey bees, there was no dilution effect of diacetin for the non-oil bee Melitta haemorrhoidalis (t = 1.00, df = 4, P = 0.37).

Bottom Line: The structural and obvious biosynthetic similarity between diacetin and associated floral oils make it a reliable cue for oil-collecting bees.It is easily perceived by oil bees, but can't be detected by other potential pollinators.Therefore, diacetin represents the first demonstrated private communication channel in a pollination system.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Ecology and Evolution, University of Salzburg, Hellbrunnerstr. 34, 5020 Salzburg, Austria [2] Department of Plant Systematics, University of Bayreuth, 95440 Bayreuth.

ABSTRACT
The interaction between floral oil secreting plants and oil-collecting bees is one of the most specialized of all pollination mutualisms. Yet, the specific stimuli used by the bees to locate their host flowers have remained elusive. This study identifies diacetin, a volatile acetylated glycerol, as a floral signal compound shared by unrelated oil plants from around the globe. Electrophysiological measurements of antennae and behavioural assays identified diacetin as the key volatile used by oil-collecting bees to locate their host flowers. Furthermore, electrophysiological measurements indicate that only oil-collecting bees are capable of detecting diacetin. The structural and obvious biosynthetic similarity between diacetin and associated floral oils make it a reliable cue for oil-collecting bees. It is easily perceived by oil bees, but can't be detected by other potential pollinators. Therefore, diacetin represents the first demonstrated private communication channel in a pollination system.

No MeSH data available.