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Diacetin, a reliable cue and private communication channel in a specialized pollination system.

Schäffler I, Steiner KE, Haid M, van Berkel SS, Gerlach G, Johnson SD, Wessjohann L, Dötterl S - Sci Rep (2015)

Bottom Line: The structural and obvious biosynthetic similarity between diacetin and associated floral oils make it a reliable cue for oil-collecting bees.It is easily perceived by oil bees, but can't be detected by other potential pollinators.Therefore, diacetin represents the first demonstrated private communication channel in a pollination system.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Ecology and Evolution, University of Salzburg, Hellbrunnerstr. 34, 5020 Salzburg, Austria [2] Department of Plant Systematics, University of Bayreuth, 95440 Bayreuth.

ABSTRACT
The interaction between floral oil secreting plants and oil-collecting bees is one of the most specialized of all pollination mutualisms. Yet, the specific stimuli used by the bees to locate their host flowers have remained elusive. This study identifies diacetin, a volatile acetylated glycerol, as a floral signal compound shared by unrelated oil plants from around the globe. Electrophysiological measurements of antennae and behavioural assays identified diacetin as the key volatile used by oil-collecting bees to locate their host flowers. Furthermore, electrophysiological measurements indicate that only oil-collecting bees are capable of detecting diacetin. The structural and obvious biosynthetic similarity between diacetin and associated floral oils make it a reliable cue for oil-collecting bees. It is easily perceived by oil bees, but can't be detected by other potential pollinators. Therefore, diacetin represents the first demonstrated private communication channel in a pollination system.

No MeSH data available.


Electroantennographic responses of Macropis fulvipes oil bees to scents of different oil flowers.GC-FID (black line) and GC-EAD responses of M. fulvipes antennae (inverted blue line) to floral extracts of L. punctata (a), L. congestiflora (b), Diascia integerrima (c), and Corycium dracomontanum (d); (UNK RI 1264: unknown compound, kovats retention index 1264; m/z: 122, 78, 106, 51, 50). Plant photographs by Irmgard Schäffler (a,b) and Kim E. Steiner (c,d).
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f1: Electroantennographic responses of Macropis fulvipes oil bees to scents of different oil flowers.GC-FID (black line) and GC-EAD responses of M. fulvipes antennae (inverted blue line) to floral extracts of L. punctata (a), L. congestiflora (b), Diascia integerrima (c), and Corycium dracomontanum (d); (UNK RI 1264: unknown compound, kovats retention index 1264; m/z: 122, 78, 106, 51, 50). Plant photographs by Irmgard Schäffler (a,b) and Kim E. Steiner (c,d).

Mentions: In the GC-EAD analyses with antennae of M. fulvipes and scent samples collected from four different oil plants, we found only one EAD-active compound, diacetin, that occurred in all of these plant species (Fig. 1). Two EAD-active compounds (heptanoic acid, 2-tridecanone) occurred in three of the plant species, whereas six compounds (1-hydroxy-1-phenyl-2-propanone, triacetin, (E)-2-dodecenal, 3,5-dimethoxytoluene, 4-hydroxy-3-methoxystyrene, UNK RI: 1264) occurred in one of the four plant species.


Diacetin, a reliable cue and private communication channel in a specialized pollination system.

Schäffler I, Steiner KE, Haid M, van Berkel SS, Gerlach G, Johnson SD, Wessjohann L, Dötterl S - Sci Rep (2015)

Electroantennographic responses of Macropis fulvipes oil bees to scents of different oil flowers.GC-FID (black line) and GC-EAD responses of M. fulvipes antennae (inverted blue line) to floral extracts of L. punctata (a), L. congestiflora (b), Diascia integerrima (c), and Corycium dracomontanum (d); (UNK RI 1264: unknown compound, kovats retention index 1264; m/z: 122, 78, 106, 51, 50). Plant photographs by Irmgard Schäffler (a,b) and Kim E. Steiner (c,d).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526864&req=5

f1: Electroantennographic responses of Macropis fulvipes oil bees to scents of different oil flowers.GC-FID (black line) and GC-EAD responses of M. fulvipes antennae (inverted blue line) to floral extracts of L. punctata (a), L. congestiflora (b), Diascia integerrima (c), and Corycium dracomontanum (d); (UNK RI 1264: unknown compound, kovats retention index 1264; m/z: 122, 78, 106, 51, 50). Plant photographs by Irmgard Schäffler (a,b) and Kim E. Steiner (c,d).
Mentions: In the GC-EAD analyses with antennae of M. fulvipes and scent samples collected from four different oil plants, we found only one EAD-active compound, diacetin, that occurred in all of these plant species (Fig. 1). Two EAD-active compounds (heptanoic acid, 2-tridecanone) occurred in three of the plant species, whereas six compounds (1-hydroxy-1-phenyl-2-propanone, triacetin, (E)-2-dodecenal, 3,5-dimethoxytoluene, 4-hydroxy-3-methoxystyrene, UNK RI: 1264) occurred in one of the four plant species.

Bottom Line: The structural and obvious biosynthetic similarity between diacetin and associated floral oils make it a reliable cue for oil-collecting bees.It is easily perceived by oil bees, but can't be detected by other potential pollinators.Therefore, diacetin represents the first demonstrated private communication channel in a pollination system.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Ecology and Evolution, University of Salzburg, Hellbrunnerstr. 34, 5020 Salzburg, Austria [2] Department of Plant Systematics, University of Bayreuth, 95440 Bayreuth.

ABSTRACT
The interaction between floral oil secreting plants and oil-collecting bees is one of the most specialized of all pollination mutualisms. Yet, the specific stimuli used by the bees to locate their host flowers have remained elusive. This study identifies diacetin, a volatile acetylated glycerol, as a floral signal compound shared by unrelated oil plants from around the globe. Electrophysiological measurements of antennae and behavioural assays identified diacetin as the key volatile used by oil-collecting bees to locate their host flowers. Furthermore, electrophysiological measurements indicate that only oil-collecting bees are capable of detecting diacetin. The structural and obvious biosynthetic similarity between diacetin and associated floral oils make it a reliable cue for oil-collecting bees. It is easily perceived by oil bees, but can't be detected by other potential pollinators. Therefore, diacetin represents the first demonstrated private communication channel in a pollination system.

No MeSH data available.