Limits...
High nutrient availability reduces the diversity and stability of the equine caecal microbiota.

Hansen NC, Avershina E, Mydland LT, Næsset JA, Austbø D, Moen B, Måge I, Rudi K - Microb. Ecol. Health Dis. (2015)

Bottom Line: It is well known that nutrient availability can alter the gut microbiota composition, while the effect on diversity and temporal stability remains largely unknown.These observations concur with general ecological theories, suggesting a stabilising effect of biological diversity and that high nutrient availability has a destabilising effect through reduced diversity.Nutrient availability does not only change the composition but also the ecology of the caecal microbiota.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, Aas, Norway.

ABSTRACT

Background: It is well known that nutrient availability can alter the gut microbiota composition, while the effect on diversity and temporal stability remains largely unknown.

Methods: Here we address the equine caecal microbiota temporal stability, diversity, and functionality in response to diets with different levels of nutrient availability. Hay (low and slower nutrient availability) versus a mixture of hay and whole oats (high and more rapid nutrient availability) were used as experimental diets.

Results: We found major effects on the microbiota despite that the caecal pH was far from sub-clinical acidosis. We found that the low nutrient availability diet was associated with a higher level of both diversity and temporal stability of the caecal microbiota than the high nutrient availability diet. These observations concur with general ecological theories, suggesting a stabilising effect of biological diversity and that high nutrient availability has a destabilising effect through reduced diversity.

Conclusion: Nutrient availability does not only change the composition but also the ecology of the caecal microbiota.

No MeSH data available.


Related in: MedlinePlus

Community stability and diversity. (a) Stability index was calculated for each time series including the five resolved MCR components. Mean of community stability in caecum of horses fed a hay diet compared to horses fed an oat diet were significantly different (p<0.01). (b) Simpsons index was calculated for each sample and the mean value was calculated for each diet and found to be significantly different (p<0.01). Error bars represent standard deviations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4526772&req=5

Figure 0005: Community stability and diversity. (a) Stability index was calculated for each time series including the five resolved MCR components. Mean of community stability in caecum of horses fed a hay diet compared to horses fed an oat diet were significantly different (p<0.01). (b) Simpsons index was calculated for each sample and the mean value was calculated for each diet and found to be significantly different (p<0.01). Error bars represent standard deviations.

Mentions: For the stability analyses, we investigated the individual differences for each time point using the mixed sequence data. The reason why we did not use the pyrosequencing data for stability analyses is that we did not have complete time series. The mixed sequence data revealed both major individual and dietary differences (Supplementary Fig. 2). There was a statistically significant difference in the temporal stability index of the microbiota for the two diets, with the stability index being highest for the hay diet (Fig. 5a).


High nutrient availability reduces the diversity and stability of the equine caecal microbiota.

Hansen NC, Avershina E, Mydland LT, Næsset JA, Austbø D, Moen B, Måge I, Rudi K - Microb. Ecol. Health Dis. (2015)

Community stability and diversity. (a) Stability index was calculated for each time series including the five resolved MCR components. Mean of community stability in caecum of horses fed a hay diet compared to horses fed an oat diet were significantly different (p<0.01). (b) Simpsons index was calculated for each sample and the mean value was calculated for each diet and found to be significantly different (p<0.01). Error bars represent standard deviations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526772&req=5

Figure 0005: Community stability and diversity. (a) Stability index was calculated for each time series including the five resolved MCR components. Mean of community stability in caecum of horses fed a hay diet compared to horses fed an oat diet were significantly different (p<0.01). (b) Simpsons index was calculated for each sample and the mean value was calculated for each diet and found to be significantly different (p<0.01). Error bars represent standard deviations.
Mentions: For the stability analyses, we investigated the individual differences for each time point using the mixed sequence data. The reason why we did not use the pyrosequencing data for stability analyses is that we did not have complete time series. The mixed sequence data revealed both major individual and dietary differences (Supplementary Fig. 2). There was a statistically significant difference in the temporal stability index of the microbiota for the two diets, with the stability index being highest for the hay diet (Fig. 5a).

Bottom Line: It is well known that nutrient availability can alter the gut microbiota composition, while the effect on diversity and temporal stability remains largely unknown.These observations concur with general ecological theories, suggesting a stabilising effect of biological diversity and that high nutrient availability has a destabilising effect through reduced diversity.Nutrient availability does not only change the composition but also the ecology of the caecal microbiota.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, Aas, Norway.

ABSTRACT

Background: It is well known that nutrient availability can alter the gut microbiota composition, while the effect on diversity and temporal stability remains largely unknown.

Methods: Here we address the equine caecal microbiota temporal stability, diversity, and functionality in response to diets with different levels of nutrient availability. Hay (low and slower nutrient availability) versus a mixture of hay and whole oats (high and more rapid nutrient availability) were used as experimental diets.

Results: We found major effects on the microbiota despite that the caecal pH was far from sub-clinical acidosis. We found that the low nutrient availability diet was associated with a higher level of both diversity and temporal stability of the caecal microbiota than the high nutrient availability diet. These observations concur with general ecological theories, suggesting a stabilising effect of biological diversity and that high nutrient availability has a destabilising effect through reduced diversity.

Conclusion: Nutrient availability does not only change the composition but also the ecology of the caecal microbiota.

No MeSH data available.


Related in: MedlinePlus