Limits...
High nutrient availability reduces the diversity and stability of the equine caecal microbiota.

Hansen NC, Avershina E, Mydland LT, Næsset JA, Austbø D, Moen B, Måge I, Rudi K - Microb. Ecol. Health Dis. (2015)

Bottom Line: It is well known that nutrient availability can alter the gut microbiota composition, while the effect on diversity and temporal stability remains largely unknown.These observations concur with general ecological theories, suggesting a stabilising effect of biological diversity and that high nutrient availability has a destabilising effect through reduced diversity.Nutrient availability does not only change the composition but also the ecology of the caecal microbiota.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, Aas, Norway.

ABSTRACT

Background: It is well known that nutrient availability can alter the gut microbiota composition, while the effect on diversity and temporal stability remains largely unknown.

Methods: Here we address the equine caecal microbiota temporal stability, diversity, and functionality in response to diets with different levels of nutrient availability. Hay (low and slower nutrient availability) versus a mixture of hay and whole oats (high and more rapid nutrient availability) were used as experimental diets.

Results: We found major effects on the microbiota despite that the caecal pH was far from sub-clinical acidosis. We found that the low nutrient availability diet was associated with a higher level of both diversity and temporal stability of the caecal microbiota than the high nutrient availability diet. These observations concur with general ecological theories, suggesting a stabilising effect of biological diversity and that high nutrient availability has a destabilising effect through reduced diversity.

Conclusion: Nutrient availability does not only change the composition but also the ecology of the caecal microbiota.

No MeSH data available.


Related in: MedlinePlus

Postprandial microbial concentration profiles in caecum of horses fed the hay diet (black) and the oat diet (red). Estimated Multivariate Curve Resolution (MCR) concentration profiles obtained PCR amplification of the variable V3 and V4 regions of 16S rRNA gene, direct sequencing, and automatic capillary electrophoresis. Main resolved bacterial groups of the mixed sequences at the family level were Porphyromonadaceae, Prevotellaceae, and Lachnospiraceae. Two of the components (Mixed 1 and 2) could not be resolved at a family level, but belong to the phylum Proteobacter. Error bars represent standard deviations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4526772&req=5

Figure 0004: Postprandial microbial concentration profiles in caecum of horses fed the hay diet (black) and the oat diet (red). Estimated Multivariate Curve Resolution (MCR) concentration profiles obtained PCR amplification of the variable V3 and V4 regions of 16S rRNA gene, direct sequencing, and automatic capillary electrophoresis. Main resolved bacterial groups of the mixed sequences at the family level were Porphyromonadaceae, Prevotellaceae, and Lachnospiraceae. Two of the components (Mixed 1 and 2) could not be resolved at a family level, but belong to the phylum Proteobacter. Error bars represent standard deviations.

Mentions: Caecal fluid was collected 0, 1, 2, 3, 4, 5, 6, 7, 8, and 9 hours after the morning feeding. Each time series (10 samples) were collected from each horse in two repetitions on days 18 and 20 for both the first and second experimental period. Caecal samples for microbiota analyses were immediately conserved in ethanol (diluted 1:4 in ethanol) and stored at −20°C until extraction of DNA. Samples for NH3 and VFA analyses (9 ml) were immediately conserved in 1ml 12 M formic acid and stored at 4°C until analysis (Fig. 2). The first time series from Horse 1 fed the hay diet is suspected to be biased due to oxygen leakage through the silicone rubber plug that was sealing the caecal cannula; thus, these results were excluded from statistical analyses and results shown in Figs. 2 and 4.


High nutrient availability reduces the diversity and stability of the equine caecal microbiota.

Hansen NC, Avershina E, Mydland LT, Næsset JA, Austbø D, Moen B, Måge I, Rudi K - Microb. Ecol. Health Dis. (2015)

Postprandial microbial concentration profiles in caecum of horses fed the hay diet (black) and the oat diet (red). Estimated Multivariate Curve Resolution (MCR) concentration profiles obtained PCR amplification of the variable V3 and V4 regions of 16S rRNA gene, direct sequencing, and automatic capillary electrophoresis. Main resolved bacterial groups of the mixed sequences at the family level were Porphyromonadaceae, Prevotellaceae, and Lachnospiraceae. Two of the components (Mixed 1 and 2) could not be resolved at a family level, but belong to the phylum Proteobacter. Error bars represent standard deviations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526772&req=5

Figure 0004: Postprandial microbial concentration profiles in caecum of horses fed the hay diet (black) and the oat diet (red). Estimated Multivariate Curve Resolution (MCR) concentration profiles obtained PCR amplification of the variable V3 and V4 regions of 16S rRNA gene, direct sequencing, and automatic capillary electrophoresis. Main resolved bacterial groups of the mixed sequences at the family level were Porphyromonadaceae, Prevotellaceae, and Lachnospiraceae. Two of the components (Mixed 1 and 2) could not be resolved at a family level, but belong to the phylum Proteobacter. Error bars represent standard deviations.
Mentions: Caecal fluid was collected 0, 1, 2, 3, 4, 5, 6, 7, 8, and 9 hours after the morning feeding. Each time series (10 samples) were collected from each horse in two repetitions on days 18 and 20 for both the first and second experimental period. Caecal samples for microbiota analyses were immediately conserved in ethanol (diluted 1:4 in ethanol) and stored at −20°C until extraction of DNA. Samples for NH3 and VFA analyses (9 ml) were immediately conserved in 1ml 12 M formic acid and stored at 4°C until analysis (Fig. 2). The first time series from Horse 1 fed the hay diet is suspected to be biased due to oxygen leakage through the silicone rubber plug that was sealing the caecal cannula; thus, these results were excluded from statistical analyses and results shown in Figs. 2 and 4.

Bottom Line: It is well known that nutrient availability can alter the gut microbiota composition, while the effect on diversity and temporal stability remains largely unknown.These observations concur with general ecological theories, suggesting a stabilising effect of biological diversity and that high nutrient availability has a destabilising effect through reduced diversity.Nutrient availability does not only change the composition but also the ecology of the caecal microbiota.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, Aas, Norway.

ABSTRACT

Background: It is well known that nutrient availability can alter the gut microbiota composition, while the effect on diversity and temporal stability remains largely unknown.

Methods: Here we address the equine caecal microbiota temporal stability, diversity, and functionality in response to diets with different levels of nutrient availability. Hay (low and slower nutrient availability) versus a mixture of hay and whole oats (high and more rapid nutrient availability) were used as experimental diets.

Results: We found major effects on the microbiota despite that the caecal pH was far from sub-clinical acidosis. We found that the low nutrient availability diet was associated with a higher level of both diversity and temporal stability of the caecal microbiota than the high nutrient availability diet. These observations concur with general ecological theories, suggesting a stabilising effect of biological diversity and that high nutrient availability has a destabilising effect through reduced diversity.

Conclusion: Nutrient availability does not only change the composition but also the ecology of the caecal microbiota.

No MeSH data available.


Related in: MedlinePlus