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High nutrient availability reduces the diversity and stability of the equine caecal microbiota.

Hansen NC, Avershina E, Mydland LT, Næsset JA, Austbø D, Moen B, Måge I, Rudi K - Microb. Ecol. Health Dis. (2015)

Bottom Line: It is well known that nutrient availability can alter the gut microbiota composition, while the effect on diversity and temporal stability remains largely unknown.These observations concur with general ecological theories, suggesting a stabilising effect of biological diversity and that high nutrient availability has a destabilising effect through reduced diversity.Nutrient availability does not only change the composition but also the ecology of the caecal microbiota.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, Aas, Norway.

ABSTRACT

Background: It is well known that nutrient availability can alter the gut microbiota composition, while the effect on diversity and temporal stability remains largely unknown.

Methods: Here we address the equine caecal microbiota temporal stability, diversity, and functionality in response to diets with different levels of nutrient availability. Hay (low and slower nutrient availability) versus a mixture of hay and whole oats (high and more rapid nutrient availability) were used as experimental diets.

Results: We found major effects on the microbiota despite that the caecal pH was far from sub-clinical acidosis. We found that the low nutrient availability diet was associated with a higher level of both diversity and temporal stability of the caecal microbiota than the high nutrient availability diet. These observations concur with general ecological theories, suggesting a stabilising effect of biological diversity and that high nutrient availability has a destabilising effect through reduced diversity.

Conclusion: Nutrient availability does not only change the composition but also the ecology of the caecal microbiota.

No MeSH data available.


Related in: MedlinePlus

Caecal VFA and NH3-N. Temporal differences. Head titles indicate VFA, NH3N, and pH values. Caecal measurements related to the oat diet are marked with ◯, and caecal measurements related to a hay diet are marked with ■. Each time point represents mean values. Error bars represent standard deviations.
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Figure 0002: Caecal VFA and NH3-N. Temporal differences. Head titles indicate VFA, NH3N, and pH values. Caecal measurements related to the oat diet are marked with ◯, and caecal measurements related to a hay diet are marked with ■. Each time point represents mean values. Error bars represent standard deviations.

Mentions: Caecal fluid was collected 0, 1, 2, 3, 4, 5, 6, 7, 8, and 9 hours after the morning feeding. Each time series (10 samples) were collected from each horse in two repetitions on days 18 and 20 for both the first and second experimental period. Caecal samples for microbiota analyses were immediately conserved in ethanol (diluted 1:4 in ethanol) and stored at −20°C until extraction of DNA. Samples for NH3 and VFA analyses (9 ml) were immediately conserved in 1ml 12 M formic acid and stored at 4°C until analysis (Fig. 2). The first time series from Horse 1 fed the hay diet is suspected to be biased due to oxygen leakage through the silicone rubber plug that was sealing the caecal cannula; thus, these results were excluded from statistical analyses and results shown in Figs. 2 and 4.


High nutrient availability reduces the diversity and stability of the equine caecal microbiota.

Hansen NC, Avershina E, Mydland LT, Næsset JA, Austbø D, Moen B, Måge I, Rudi K - Microb. Ecol. Health Dis. (2015)

Caecal VFA and NH3-N. Temporal differences. Head titles indicate VFA, NH3N, and pH values. Caecal measurements related to the oat diet are marked with ◯, and caecal measurements related to a hay diet are marked with ■. Each time point represents mean values. Error bars represent standard deviations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526772&req=5

Figure 0002: Caecal VFA and NH3-N. Temporal differences. Head titles indicate VFA, NH3N, and pH values. Caecal measurements related to the oat diet are marked with ◯, and caecal measurements related to a hay diet are marked with ■. Each time point represents mean values. Error bars represent standard deviations.
Mentions: Caecal fluid was collected 0, 1, 2, 3, 4, 5, 6, 7, 8, and 9 hours after the morning feeding. Each time series (10 samples) were collected from each horse in two repetitions on days 18 and 20 for both the first and second experimental period. Caecal samples for microbiota analyses were immediately conserved in ethanol (diluted 1:4 in ethanol) and stored at −20°C until extraction of DNA. Samples for NH3 and VFA analyses (9 ml) were immediately conserved in 1ml 12 M formic acid and stored at 4°C until analysis (Fig. 2). The first time series from Horse 1 fed the hay diet is suspected to be biased due to oxygen leakage through the silicone rubber plug that was sealing the caecal cannula; thus, these results were excluded from statistical analyses and results shown in Figs. 2 and 4.

Bottom Line: It is well known that nutrient availability can alter the gut microbiota composition, while the effect on diversity and temporal stability remains largely unknown.These observations concur with general ecological theories, suggesting a stabilising effect of biological diversity and that high nutrient availability has a destabilising effect through reduced diversity.Nutrient availability does not only change the composition but also the ecology of the caecal microbiota.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, Aas, Norway.

ABSTRACT

Background: It is well known that nutrient availability can alter the gut microbiota composition, while the effect on diversity and temporal stability remains largely unknown.

Methods: Here we address the equine caecal microbiota temporal stability, diversity, and functionality in response to diets with different levels of nutrient availability. Hay (low and slower nutrient availability) versus a mixture of hay and whole oats (high and more rapid nutrient availability) were used as experimental diets.

Results: We found major effects on the microbiota despite that the caecal pH was far from sub-clinical acidosis. We found that the low nutrient availability diet was associated with a higher level of both diversity and temporal stability of the caecal microbiota than the high nutrient availability diet. These observations concur with general ecological theories, suggesting a stabilising effect of biological diversity and that high nutrient availability has a destabilising effect through reduced diversity.

Conclusion: Nutrient availability does not only change the composition but also the ecology of the caecal microbiota.

No MeSH data available.


Related in: MedlinePlus