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Glutathione-induced drought stress tolerance in mung bean: coordinated roles of the antioxidant defence and methylglyoxal detoxification systems.

Nahar K, Hasanuzzaman M, Alam MM, Fujita M - AoB Plants (2015)

Bottom Line: Drought stress decreased seedling dry weight and leaf area; resulted in oxidative stress as evidenced by histochemical detection of hydrogen peroxide (H2O2) and [Formula: see text] in the leaves; increased lipid peroxidation (malondialdehyde), reactive oxygen species like H2O2 content and [Formula: see text] generation rate and lipoxygenase activity; and increased the MG level.Drought decreased leaf succulence, leaf chlorophyll and relative water content (RWC); increased proline (Pro); decreased ascorbate (AsA); increased endogenous GSH and glutathione disulfide (GSSG) content; decreased the GSH/GSSG ratio; increased ascorbate peroxidase and glutathione S-transferase activities; and decreased the activities of monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR) and catalase.In contrast to drought stress alone, exogenous GSH enhanced most of the components of the antioxidant and glyoxalase systems in drought-affected mung bean seedlings at 24 h, but GSH did not significantly affect AsA, Pro, RWC, leaf succulence and the activities of Gly I and DHAR after 48 h of stress.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan Department of Agricultural Botany, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, Bangladesh.

No MeSH data available.


Related in: MedlinePlus

(A and B) Histochemical localization of H2O2 in leaves of mung bean seedlings. Here C, G, D and D + G indicate control, exogenous glutathione (GSH, 1 mM), drought stress (−0.7 MPa) and drought stress (−0.7 MPa) + exogenous glutathione (GSH, 1 mM), respectively. (C) Histochemical localization of H2O2 in leaves of drought-treated plants stained in 10 mM ascorbate where E and F indicate 24 and 48 h drought (control) treatment, respectively.
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PLV069F1: (A and B) Histochemical localization of H2O2 in leaves of mung bean seedlings. Here C, G, D and D + G indicate control, exogenous glutathione (GSH, 1 mM), drought stress (−0.7 MPa) and drought stress (−0.7 MPa) + exogenous glutathione (GSH, 1 mM), respectively. (C) Histochemical localization of H2O2 in leaves of drought-treated plants stained in 10 mM ascorbate where E and F indicate 24 and 48 h drought (control) treatment, respectively.

Mentions: Histochemical staining was performed to localize H2O2 and in the leaves of the mung bean seedlings (Figs 1 and 2). A significant increase in the accumulation of H2O2, indicated by brown spots (Fig. 1B), and accumulation of , indicated by dark blue spots (Fig. 2B), were noticed in the leaves of the mung bean seedlings under drought stress, and more spots on the leaves were evident after 48 h. Incubated leaves in 10 mM ascorbate or 10 mM MnCl2 removed the H2O2 or staining, respectively (Figs 1C and 2C), which indicated the specificity of staining. Hydrogen peroxide was suppressed by adding 10 mm ascorbic acid. The production of was suppressed by adding 10 mm MnCl2, which is considered a removing agent of (Hernández et al. 2001). However, GSH addition with drought stress reduced the spots of from the leaves. But exogenous GSH addition could not reduce the spots of H2O2 from mung bean leaves.Figure 1.


Glutathione-induced drought stress tolerance in mung bean: coordinated roles of the antioxidant defence and methylglyoxal detoxification systems.

Nahar K, Hasanuzzaman M, Alam MM, Fujita M - AoB Plants (2015)

(A and B) Histochemical localization of H2O2 in leaves of mung bean seedlings. Here C, G, D and D + G indicate control, exogenous glutathione (GSH, 1 mM), drought stress (−0.7 MPa) and drought stress (−0.7 MPa) + exogenous glutathione (GSH, 1 mM), respectively. (C) Histochemical localization of H2O2 in leaves of drought-treated plants stained in 10 mM ascorbate where E and F indicate 24 and 48 h drought (control) treatment, respectively.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526754&req=5

PLV069F1: (A and B) Histochemical localization of H2O2 in leaves of mung bean seedlings. Here C, G, D and D + G indicate control, exogenous glutathione (GSH, 1 mM), drought stress (−0.7 MPa) and drought stress (−0.7 MPa) + exogenous glutathione (GSH, 1 mM), respectively. (C) Histochemical localization of H2O2 in leaves of drought-treated plants stained in 10 mM ascorbate where E and F indicate 24 and 48 h drought (control) treatment, respectively.
Mentions: Histochemical staining was performed to localize H2O2 and in the leaves of the mung bean seedlings (Figs 1 and 2). A significant increase in the accumulation of H2O2, indicated by brown spots (Fig. 1B), and accumulation of , indicated by dark blue spots (Fig. 2B), were noticed in the leaves of the mung bean seedlings under drought stress, and more spots on the leaves were evident after 48 h. Incubated leaves in 10 mM ascorbate or 10 mM MnCl2 removed the H2O2 or staining, respectively (Figs 1C and 2C), which indicated the specificity of staining. Hydrogen peroxide was suppressed by adding 10 mm ascorbic acid. The production of was suppressed by adding 10 mm MnCl2, which is considered a removing agent of (Hernández et al. 2001). However, GSH addition with drought stress reduced the spots of from the leaves. But exogenous GSH addition could not reduce the spots of H2O2 from mung bean leaves.Figure 1.

Bottom Line: Drought stress decreased seedling dry weight and leaf area; resulted in oxidative stress as evidenced by histochemical detection of hydrogen peroxide (H2O2) and [Formula: see text] in the leaves; increased lipid peroxidation (malondialdehyde), reactive oxygen species like H2O2 content and [Formula: see text] generation rate and lipoxygenase activity; and increased the MG level.Drought decreased leaf succulence, leaf chlorophyll and relative water content (RWC); increased proline (Pro); decreased ascorbate (AsA); increased endogenous GSH and glutathione disulfide (GSSG) content; decreased the GSH/GSSG ratio; increased ascorbate peroxidase and glutathione S-transferase activities; and decreased the activities of monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR) and catalase.In contrast to drought stress alone, exogenous GSH enhanced most of the components of the antioxidant and glyoxalase systems in drought-affected mung bean seedlings at 24 h, but GSH did not significantly affect AsA, Pro, RWC, leaf succulence and the activities of Gly I and DHAR after 48 h of stress.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan Department of Agricultural Botany, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, Bangladesh.

No MeSH data available.


Related in: MedlinePlus