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Comparison of the Transcriptional Profiles of Melanocytes from Dark and Light Skinned Individuals under Basal Conditions and Following Ultraviolet-B Irradiation.

López S, Smith-Zubiaga I, García de Galdeano A, Boyano MD, García O, Gardeazábal J, Martinez-Cadenas C, Izagirre N, de la Rúa C, Alonso S - PLoS ONE (2015)

Bottom Line: Our results suggest that an interaction between ribosomal proteins and the P53 signaling pathway may occur in response to UVB in both DM and LM.Furthermore, the culture with KCM+ compared with KCM- had a noticeable effect on LM.This effect includes the activation of various signaling pathways such as the mTOR pathway, involved in the regulation of cell metabolism, growth, proliferation and survival.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, Physical Anthropology and Animal Physiology. University of the Basque Country UPV/EHU, Leioa, Bizkaia, Spain.

ABSTRACT
We analysed the whole-genome transcriptional profile of 6 cell lines of dark melanocytes (DM) and 6 of light melanocytes (LM) at basal conditions and after ultraviolet-B (UVB) radiation at different time points to investigate the mechanisms by which melanocytes protect human skin from the damaging effects of UVB. Further, we assessed the effect of different keratinocyte-conditioned media (KCM+ and KCM-) on melanocytes. Our results suggest that an interaction between ribosomal proteins and the P53 signaling pathway may occur in response to UVB in both DM and LM. We also observed that DM and LM show differentially expressed genes after irradiation, in particular at the first 6h after UVB. These are mainly associated with inflammatory reactions, cell survival or melanoma. Furthermore, the culture with KCM+ compared with KCM- had a noticeable effect on LM. This effect includes the activation of various signaling pathways such as the mTOR pathway, involved in the regulation of cell metabolism, growth, proliferation and survival. Finally, the comparison of the transcriptional profiles between LM and DM under basal conditions, and the application of natural selection tests in human populations allowed us to support the significant evolutionary role of MIF and ATP6V0B in the pigmentary phenotype.

No MeSH data available.


Related in: MedlinePlus

Gene expression of genes FDX6, RPL6, MDM2, TP53I3, ATP6VOB and MIF assessed by RT-qPCR.Unpaired t-test; *** p<0.0001; ** p<0.001; * p<0.01.
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pone.0134911.g004: Gene expression of genes FDX6, RPL6, MDM2, TP53I3, ATP6VOB and MIF assessed by RT-qPCR.Unpaired t-test; *** p<0.0001; ** p<0.001; * p<0.01.

Mentions: Six genes were selected for validation of the microarrays' results, which showed either a change of expression after UV treatment or a differential expression between LM and DM (ATP6VOB, TP53I3, MDM2, MIF, RPL6 and FDXR), and measured their expression levels by quantitative real-time PCR (RT-qPCR). We assessed the expression of 4 melanocytic cell lines (2 DM and 2 LM) at basal conditions and at 6 and 12 hours after UVB irradiation. The expression patterns and direction of changes of all of the genes were consistent with the microarray data (Fig 4), observing a significant increase in the expression of TP53I3, MDM2, RPL6 and FDXR in both LM and DM after UVB. The expression analysis of ATP6VOB and MIF also supported the differential expression of these genes by LM and DM, being ATP6VOB more expressed by LM, while MIF was more significantly expressed by DM (both at basal conditions and after UVB irradiation).


Comparison of the Transcriptional Profiles of Melanocytes from Dark and Light Skinned Individuals under Basal Conditions and Following Ultraviolet-B Irradiation.

López S, Smith-Zubiaga I, García de Galdeano A, Boyano MD, García O, Gardeazábal J, Martinez-Cadenas C, Izagirre N, de la Rúa C, Alonso S - PLoS ONE (2015)

Gene expression of genes FDX6, RPL6, MDM2, TP53I3, ATP6VOB and MIF assessed by RT-qPCR.Unpaired t-test; *** p<0.0001; ** p<0.001; * p<0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526690&req=5

pone.0134911.g004: Gene expression of genes FDX6, RPL6, MDM2, TP53I3, ATP6VOB and MIF assessed by RT-qPCR.Unpaired t-test; *** p<0.0001; ** p<0.001; * p<0.01.
Mentions: Six genes were selected for validation of the microarrays' results, which showed either a change of expression after UV treatment or a differential expression between LM and DM (ATP6VOB, TP53I3, MDM2, MIF, RPL6 and FDXR), and measured their expression levels by quantitative real-time PCR (RT-qPCR). We assessed the expression of 4 melanocytic cell lines (2 DM and 2 LM) at basal conditions and at 6 and 12 hours after UVB irradiation. The expression patterns and direction of changes of all of the genes were consistent with the microarray data (Fig 4), observing a significant increase in the expression of TP53I3, MDM2, RPL6 and FDXR in both LM and DM after UVB. The expression analysis of ATP6VOB and MIF also supported the differential expression of these genes by LM and DM, being ATP6VOB more expressed by LM, while MIF was more significantly expressed by DM (both at basal conditions and after UVB irradiation).

Bottom Line: Our results suggest that an interaction between ribosomal proteins and the P53 signaling pathway may occur in response to UVB in both DM and LM.Furthermore, the culture with KCM+ compared with KCM- had a noticeable effect on LM.This effect includes the activation of various signaling pathways such as the mTOR pathway, involved in the regulation of cell metabolism, growth, proliferation and survival.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, Physical Anthropology and Animal Physiology. University of the Basque Country UPV/EHU, Leioa, Bizkaia, Spain.

ABSTRACT
We analysed the whole-genome transcriptional profile of 6 cell lines of dark melanocytes (DM) and 6 of light melanocytes (LM) at basal conditions and after ultraviolet-B (UVB) radiation at different time points to investigate the mechanisms by which melanocytes protect human skin from the damaging effects of UVB. Further, we assessed the effect of different keratinocyte-conditioned media (KCM+ and KCM-) on melanocytes. Our results suggest that an interaction between ribosomal proteins and the P53 signaling pathway may occur in response to UVB in both DM and LM. We also observed that DM and LM show differentially expressed genes after irradiation, in particular at the first 6h after UVB. These are mainly associated with inflammatory reactions, cell survival or melanoma. Furthermore, the culture with KCM+ compared with KCM- had a noticeable effect on LM. This effect includes the activation of various signaling pathways such as the mTOR pathway, involved in the regulation of cell metabolism, growth, proliferation and survival. Finally, the comparison of the transcriptional profiles between LM and DM under basal conditions, and the application of natural selection tests in human populations allowed us to support the significant evolutionary role of MIF and ATP6V0B in the pigmentary phenotype.

No MeSH data available.


Related in: MedlinePlus