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Anti-Osteoarthritic Effects of the Litsea japonica Fruit in a Rat Model of Osteoarthritis Induced by Monosodium Iodoacetate.

Jeong YJ, Kim I, Cho JH, Park DW, Kwon JE, Jung MW, Meng X, Jo SM, Song HS, Cho YM, Song SM, Ham YM, Jung YH, Kim CS, Yoon WJ, Kang SC - PLoS ONE (2015)

Bottom Line: In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA.In addition, the LJFE decreased the expression of inflammatory cytokines.Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.

ABSTRACT
Osteoarthritis (OA) is a degenerative chronic disease that affects various tissues surrounding the joints, such as the subchondral bone and articular cartilage. The onset of OA is associated with uncontrolled catabolic and anabolic remodeling processes of the joints, including the cartilage and subchondral bone, to adapt to local biological and biochemical signals. In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA. Our results showed that administration of LJFE increased the bone volume and cross-section thickness, but the mean number of objects per slice in this group was lower than that in the OA control (OAC) group. In addition, the LJFE decreased the expression of inflammatory cytokines. Compared to the OAC group, the group treated with high doses of LJFE (100 and 200 mg/kg) showed a more than 80% inhibition of the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases. Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

No MeSH data available.


Related in: MedlinePlus

Levels of matrix metalloproteinase 2 (MMP-2) (A), MMP-3 (B), MMP-7 (C), MMP-9 (D), MMP-13 (E), tissue inhibitor of metalloproteinase 1 (TIMP-1) (F), and TIMP-2 (G) in the cartilage tissue.RNA was extracted from the cartilage tissue of rats with osteoarthritis, and the mRNA levels of MMPs and TIMPs were determined using real-time polymerase chain reaction (PCR). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as a house-keeping gene. The results are mean ± standard error of mean (SEM) for six rats per group (*p < 0.05, **p < 0.01 compared to NC, #p < 0.05, ##p < 0.01 compared to OAC).
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pone.0134856.g005: Levels of matrix metalloproteinase 2 (MMP-2) (A), MMP-3 (B), MMP-7 (C), MMP-9 (D), MMP-13 (E), tissue inhibitor of metalloproteinase 1 (TIMP-1) (F), and TIMP-2 (G) in the cartilage tissue.RNA was extracted from the cartilage tissue of rats with osteoarthritis, and the mRNA levels of MMPs and TIMPs were determined using real-time polymerase chain reaction (PCR). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as a house-keeping gene. The results are mean ± standard error of mean (SEM) for six rats per group (*p < 0.05, **p < 0.01 compared to NC, #p < 0.05, ##p < 0.01 compared to OAC).

Mentions: The OAC group showed a markedly higher mRNA expression of MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, TIMP-1, and TIMP-2 in the articular cartilage (Fig 5). However, compared to OAC group, the group treated with LJFE and IM showed a decrease in the mRNA expression of MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, TIMP-1, and TIMP-2 (Fig 5A–5G). High doses of LJFE (100 and 200 mg/kg) showed a significant inhibition of more than 80% of the expression of all these genes. These results indicated that LJFE directly modulated the expression of MMPs but not via TIMP stimulation. Possible explanations could be that LJFE have another active component with the MMP-like activity, thereby exerting the MMP-like activity. Extended experiments will be continued to figure out the mechanism by which LJFE inhibits the expression of MMPs.


Anti-Osteoarthritic Effects of the Litsea japonica Fruit in a Rat Model of Osteoarthritis Induced by Monosodium Iodoacetate.

Jeong YJ, Kim I, Cho JH, Park DW, Kwon JE, Jung MW, Meng X, Jo SM, Song HS, Cho YM, Song SM, Ham YM, Jung YH, Kim CS, Yoon WJ, Kang SC - PLoS ONE (2015)

Levels of matrix metalloproteinase 2 (MMP-2) (A), MMP-3 (B), MMP-7 (C), MMP-9 (D), MMP-13 (E), tissue inhibitor of metalloproteinase 1 (TIMP-1) (F), and TIMP-2 (G) in the cartilage tissue.RNA was extracted from the cartilage tissue of rats with osteoarthritis, and the mRNA levels of MMPs and TIMPs were determined using real-time polymerase chain reaction (PCR). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as a house-keeping gene. The results are mean ± standard error of mean (SEM) for six rats per group (*p < 0.05, **p < 0.01 compared to NC, #p < 0.05, ##p < 0.01 compared to OAC).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4526681&req=5

pone.0134856.g005: Levels of matrix metalloproteinase 2 (MMP-2) (A), MMP-3 (B), MMP-7 (C), MMP-9 (D), MMP-13 (E), tissue inhibitor of metalloproteinase 1 (TIMP-1) (F), and TIMP-2 (G) in the cartilage tissue.RNA was extracted from the cartilage tissue of rats with osteoarthritis, and the mRNA levels of MMPs and TIMPs were determined using real-time polymerase chain reaction (PCR). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as a house-keeping gene. The results are mean ± standard error of mean (SEM) for six rats per group (*p < 0.05, **p < 0.01 compared to NC, #p < 0.05, ##p < 0.01 compared to OAC).
Mentions: The OAC group showed a markedly higher mRNA expression of MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, TIMP-1, and TIMP-2 in the articular cartilage (Fig 5). However, compared to OAC group, the group treated with LJFE and IM showed a decrease in the mRNA expression of MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, TIMP-1, and TIMP-2 (Fig 5A–5G). High doses of LJFE (100 and 200 mg/kg) showed a significant inhibition of more than 80% of the expression of all these genes. These results indicated that LJFE directly modulated the expression of MMPs but not via TIMP stimulation. Possible explanations could be that LJFE have another active component with the MMP-like activity, thereby exerting the MMP-like activity. Extended experiments will be continued to figure out the mechanism by which LJFE inhibits the expression of MMPs.

Bottom Line: In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA.In addition, the LJFE decreased the expression of inflammatory cytokines.Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.

ABSTRACT
Osteoarthritis (OA) is a degenerative chronic disease that affects various tissues surrounding the joints, such as the subchondral bone and articular cartilage. The onset of OA is associated with uncontrolled catabolic and anabolic remodeling processes of the joints, including the cartilage and subchondral bone, to adapt to local biological and biochemical signals. In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA. Our results showed that administration of LJFE increased the bone volume and cross-section thickness, but the mean number of objects per slice in this group was lower than that in the OA control (OAC) group. In addition, the LJFE decreased the expression of inflammatory cytokines. Compared to the OAC group, the group treated with high doses of LJFE (100 and 200 mg/kg) showed a more than 80% inhibition of the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases. Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

No MeSH data available.


Related in: MedlinePlus