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Anti-Osteoarthritic Effects of the Litsea japonica Fruit in a Rat Model of Osteoarthritis Induced by Monosodium Iodoacetate.

Jeong YJ, Kim I, Cho JH, Park DW, Kwon JE, Jung MW, Meng X, Jo SM, Song HS, Cho YM, Song SM, Ham YM, Jung YH, Kim CS, Yoon WJ, Kang SC - PLoS ONE (2015)

Bottom Line: In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA.In addition, the LJFE decreased the expression of inflammatory cytokines.Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.

ABSTRACT
Osteoarthritis (OA) is a degenerative chronic disease that affects various tissues surrounding the joints, such as the subchondral bone and articular cartilage. The onset of OA is associated with uncontrolled catabolic and anabolic remodeling processes of the joints, including the cartilage and subchondral bone, to adapt to local biological and biochemical signals. In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA. Our results showed that administration of LJFE increased the bone volume and cross-section thickness, but the mean number of objects per slice in this group was lower than that in the OA control (OAC) group. In addition, the LJFE decreased the expression of inflammatory cytokines. Compared to the OAC group, the group treated with high doses of LJFE (100 and 200 mg/kg) showed a more than 80% inhibition of the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases. Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

No MeSH data available.


Related in: MedlinePlus

Levels of interleukin 6 (IL-6) (A), tumor necrosis factor-α (TNF-α) (B), and IL-1β (C) in the serum of rats with osteoarthritis.The levels of cytokines in the serum were determined using an enzyme-linked immunosorbent assay (ELISA). The results are mean ± standard error mean (SEM) for six rats per group (**p < 0.01 compared to NC, #p < 0.05, ##p < 0.01 compared to OAC).
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pone.0134856.g004: Levels of interleukin 6 (IL-6) (A), tumor necrosis factor-α (TNF-α) (B), and IL-1β (C) in the serum of rats with osteoarthritis.The levels of cytokines in the serum were determined using an enzyme-linked immunosorbent assay (ELISA). The results are mean ± standard error mean (SEM) for six rats per group (**p < 0.01 compared to NC, #p < 0.05, ##p < 0.01 compared to OAC).

Mentions: The serum levels of IL-6, IL-1β, and TNF-α were higher in the OAC group than in the NC group, and LJFE showed a significant and dose-dependent decrease in these levels, except those of IL-1β, compared to those in the OAC group (Fig 4A and 4B). Compared to the LJFE group, the OAC group did not show a dose-dependent decrease in IL-1β (Fig 4C). In particular, the group treated with 200 mg/kg of LJFE effectively inhibited the production of IL-6 (288.3 ± 11.67 pg/mL, p < 0.01), TNF-α (160.0 ± 10.00 pg/mL, p < 0.01), and IL-1β (410.0 ± 5.00 pg/mL, p < 0.05) compared to that in the OAC group (451.7 ± 18.78, 303.3 ± 13.33, and 510.0 ± 31.22 pg/mL, respectively).


Anti-Osteoarthritic Effects of the Litsea japonica Fruit in a Rat Model of Osteoarthritis Induced by Monosodium Iodoacetate.

Jeong YJ, Kim I, Cho JH, Park DW, Kwon JE, Jung MW, Meng X, Jo SM, Song HS, Cho YM, Song SM, Ham YM, Jung YH, Kim CS, Yoon WJ, Kang SC - PLoS ONE (2015)

Levels of interleukin 6 (IL-6) (A), tumor necrosis factor-α (TNF-α) (B), and IL-1β (C) in the serum of rats with osteoarthritis.The levels of cytokines in the serum were determined using an enzyme-linked immunosorbent assay (ELISA). The results are mean ± standard error mean (SEM) for six rats per group (**p < 0.01 compared to NC, #p < 0.05, ##p < 0.01 compared to OAC).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526681&req=5

pone.0134856.g004: Levels of interleukin 6 (IL-6) (A), tumor necrosis factor-α (TNF-α) (B), and IL-1β (C) in the serum of rats with osteoarthritis.The levels of cytokines in the serum were determined using an enzyme-linked immunosorbent assay (ELISA). The results are mean ± standard error mean (SEM) for six rats per group (**p < 0.01 compared to NC, #p < 0.05, ##p < 0.01 compared to OAC).
Mentions: The serum levels of IL-6, IL-1β, and TNF-α were higher in the OAC group than in the NC group, and LJFE showed a significant and dose-dependent decrease in these levels, except those of IL-1β, compared to those in the OAC group (Fig 4A and 4B). Compared to the LJFE group, the OAC group did not show a dose-dependent decrease in IL-1β (Fig 4C). In particular, the group treated with 200 mg/kg of LJFE effectively inhibited the production of IL-6 (288.3 ± 11.67 pg/mL, p < 0.01), TNF-α (160.0 ± 10.00 pg/mL, p < 0.01), and IL-1β (410.0 ± 5.00 pg/mL, p < 0.05) compared to that in the OAC group (451.7 ± 18.78, 303.3 ± 13.33, and 510.0 ± 31.22 pg/mL, respectively).

Bottom Line: In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA.In addition, the LJFE decreased the expression of inflammatory cytokines.Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.

ABSTRACT
Osteoarthritis (OA) is a degenerative chronic disease that affects various tissues surrounding the joints, such as the subchondral bone and articular cartilage. The onset of OA is associated with uncontrolled catabolic and anabolic remodeling processes of the joints, including the cartilage and subchondral bone, to adapt to local biological and biochemical signals. In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA. Our results showed that administration of LJFE increased the bone volume and cross-section thickness, but the mean number of objects per slice in this group was lower than that in the OA control (OAC) group. In addition, the LJFE decreased the expression of inflammatory cytokines. Compared to the OAC group, the group treated with high doses of LJFE (100 and 200 mg/kg) showed a more than 80% inhibition of the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases. Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

No MeSH data available.


Related in: MedlinePlus