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Anti-Osteoarthritic Effects of the Litsea japonica Fruit in a Rat Model of Osteoarthritis Induced by Monosodium Iodoacetate.

Jeong YJ, Kim I, Cho JH, Park DW, Kwon JE, Jung MW, Meng X, Jo SM, Song HS, Cho YM, Song SM, Ham YM, Jung YH, Kim CS, Yoon WJ, Kang SC - PLoS ONE (2015)

Bottom Line: In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA.In addition, the LJFE decreased the expression of inflammatory cytokines.Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.

ABSTRACT
Osteoarthritis (OA) is a degenerative chronic disease that affects various tissues surrounding the joints, such as the subchondral bone and articular cartilage. The onset of OA is associated with uncontrolled catabolic and anabolic remodeling processes of the joints, including the cartilage and subchondral bone, to adapt to local biological and biochemical signals. In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA. Our results showed that administration of LJFE increased the bone volume and cross-section thickness, but the mean number of objects per slice in this group was lower than that in the OA control (OAC) group. In addition, the LJFE decreased the expression of inflammatory cytokines. Compared to the OAC group, the group treated with high doses of LJFE (100 and 200 mg/kg) showed a more than 80% inhibition of the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases. Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

No MeSH data available.


Related in: MedlinePlus

Three-dimensional micro-computed tomography (CT) images and parameters of medial subchondral bone for the knee of the tibiae.(A) 3D micro-computed tomography (CT) images of the medial subchondral bone. (a) Normal control, (b) OA control, (c) OA+70% ethanolic extract of Litsea japonica fruit (LJFE) 50 mg/kg, (d) OA+LJFE 100 mg/kg, (e) OA+LJFE 200 mg/kg, and (f) indomethacin 2 mg/kg. (B) The medial subchondral bone parameters were monitored using a 3D micro-CT analysis program. The medial subchondral bone parameters assessed were as follows: (a) bone volume (BV, mm3), (b) mean number of objects per slice (Obj.N.), and (c) cross-sectional thickness (Cs.Th, mm). The results are mean ± standard error of mean (SEM) for six rats per group (**p < 0.01 compared to NC, #p < 0.05, ##p < 0.01 compared to OAC).
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pone.0134856.g003: Three-dimensional micro-computed tomography (CT) images and parameters of medial subchondral bone for the knee of the tibiae.(A) 3D micro-computed tomography (CT) images of the medial subchondral bone. (a) Normal control, (b) OA control, (c) OA+70% ethanolic extract of Litsea japonica fruit (LJFE) 50 mg/kg, (d) OA+LJFE 100 mg/kg, (e) OA+LJFE 200 mg/kg, and (f) indomethacin 2 mg/kg. (B) The medial subchondral bone parameters were monitored using a 3D micro-CT analysis program. The medial subchondral bone parameters assessed were as follows: (a) bone volume (BV, mm3), (b) mean number of objects per slice (Obj.N.), and (c) cross-sectional thickness (Cs.Th, mm). The results are mean ± standard error of mean (SEM) for six rats per group (**p < 0.01 compared to NC, #p < 0.05, ##p < 0.01 compared to OAC).

Mentions: The medial subchondral bone showed structural characteristics similar to the lateral subchondral bone (Fig 3A). The BV and Cs.Th were significantly higher and the Obj.N. was significantly lower in the groups treated with LJFE and IM than in the OAC group (Fig 3B). Compared to the OAC group (0.8 ± 0.10 mm3 and 0.2 ± 0.03 mm, respectively), the group treated with 200 mg/kg of LJFE showed a marked increase in BV (1.9 ± 0.27 mm3, p < 0.05) and Cs.Th (0.3 ± 0.01 mm, p < 0.05), whereas the Obj.N. in this group (1.5 ± 0.13, p < 0.01) was lower than that in the OAC group (2.2 ± 0.11) (Fig 3B). Thus, treatment with LJFE (50, 100, and 200 mg/kg) showed significant and dose-dependent effects on MIA-induced architectural deterioration of the subchondral bone.


Anti-Osteoarthritic Effects of the Litsea japonica Fruit in a Rat Model of Osteoarthritis Induced by Monosodium Iodoacetate.

Jeong YJ, Kim I, Cho JH, Park DW, Kwon JE, Jung MW, Meng X, Jo SM, Song HS, Cho YM, Song SM, Ham YM, Jung YH, Kim CS, Yoon WJ, Kang SC - PLoS ONE (2015)

Three-dimensional micro-computed tomography (CT) images and parameters of medial subchondral bone for the knee of the tibiae.(A) 3D micro-computed tomography (CT) images of the medial subchondral bone. (a) Normal control, (b) OA control, (c) OA+70% ethanolic extract of Litsea japonica fruit (LJFE) 50 mg/kg, (d) OA+LJFE 100 mg/kg, (e) OA+LJFE 200 mg/kg, and (f) indomethacin 2 mg/kg. (B) The medial subchondral bone parameters were monitored using a 3D micro-CT analysis program. The medial subchondral bone parameters assessed were as follows: (a) bone volume (BV, mm3), (b) mean number of objects per slice (Obj.N.), and (c) cross-sectional thickness (Cs.Th, mm). The results are mean ± standard error of mean (SEM) for six rats per group (**p < 0.01 compared to NC, #p < 0.05, ##p < 0.01 compared to OAC).
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4526681&req=5

pone.0134856.g003: Three-dimensional micro-computed tomography (CT) images and parameters of medial subchondral bone for the knee of the tibiae.(A) 3D micro-computed tomography (CT) images of the medial subchondral bone. (a) Normal control, (b) OA control, (c) OA+70% ethanolic extract of Litsea japonica fruit (LJFE) 50 mg/kg, (d) OA+LJFE 100 mg/kg, (e) OA+LJFE 200 mg/kg, and (f) indomethacin 2 mg/kg. (B) The medial subchondral bone parameters were monitored using a 3D micro-CT analysis program. The medial subchondral bone parameters assessed were as follows: (a) bone volume (BV, mm3), (b) mean number of objects per slice (Obj.N.), and (c) cross-sectional thickness (Cs.Th, mm). The results are mean ± standard error of mean (SEM) for six rats per group (**p < 0.01 compared to NC, #p < 0.05, ##p < 0.01 compared to OAC).
Mentions: The medial subchondral bone showed structural characteristics similar to the lateral subchondral bone (Fig 3A). The BV and Cs.Th were significantly higher and the Obj.N. was significantly lower in the groups treated with LJFE and IM than in the OAC group (Fig 3B). Compared to the OAC group (0.8 ± 0.10 mm3 and 0.2 ± 0.03 mm, respectively), the group treated with 200 mg/kg of LJFE showed a marked increase in BV (1.9 ± 0.27 mm3, p < 0.05) and Cs.Th (0.3 ± 0.01 mm, p < 0.05), whereas the Obj.N. in this group (1.5 ± 0.13, p < 0.01) was lower than that in the OAC group (2.2 ± 0.11) (Fig 3B). Thus, treatment with LJFE (50, 100, and 200 mg/kg) showed significant and dose-dependent effects on MIA-induced architectural deterioration of the subchondral bone.

Bottom Line: In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA.In addition, the LJFE decreased the expression of inflammatory cytokines.Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.

ABSTRACT
Osteoarthritis (OA) is a degenerative chronic disease that affects various tissues surrounding the joints, such as the subchondral bone and articular cartilage. The onset of OA is associated with uncontrolled catabolic and anabolic remodeling processes of the joints, including the cartilage and subchondral bone, to adapt to local biological and biochemical signals. In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA. Our results showed that administration of LJFE increased the bone volume and cross-section thickness, but the mean number of objects per slice in this group was lower than that in the OA control (OAC) group. In addition, the LJFE decreased the expression of inflammatory cytokines. Compared to the OAC group, the group treated with high doses of LJFE (100 and 200 mg/kg) showed a more than 80% inhibition of the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases. Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

No MeSH data available.


Related in: MedlinePlus