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Anti-Osteoarthritic Effects of the Litsea japonica Fruit in a Rat Model of Osteoarthritis Induced by Monosodium Iodoacetate.

Jeong YJ, Kim I, Cho JH, Park DW, Kwon JE, Jung MW, Meng X, Jo SM, Song HS, Cho YM, Song SM, Ham YM, Jung YH, Kim CS, Yoon WJ, Kang SC - PLoS ONE (2015)

Bottom Line: In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA.In addition, the LJFE decreased the expression of inflammatory cytokines.Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.

ABSTRACT
Osteoarthritis (OA) is a degenerative chronic disease that affects various tissues surrounding the joints, such as the subchondral bone and articular cartilage. The onset of OA is associated with uncontrolled catabolic and anabolic remodeling processes of the joints, including the cartilage and subchondral bone, to adapt to local biological and biochemical signals. In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA. Our results showed that administration of LJFE increased the bone volume and cross-section thickness, but the mean number of objects per slice in this group was lower than that in the OA control (OAC) group. In addition, the LJFE decreased the expression of inflammatory cytokines. Compared to the OAC group, the group treated with high doses of LJFE (100 and 200 mg/kg) showed a more than 80% inhibition of the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases. Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

No MeSH data available.


Related in: MedlinePlus

Three-dimensional micro-computed tomography images and parameters of lateral subchondral bone of the knee of the tibiae.(A) 3D micro-computed tomography (CT) images of the lateral subchondral bone. (a) Normal control, (b) OA control, (c) OA+70% ethanolic extract of Litsea japonica fruit (LJFE) 50 mg/kg, (d) OA+LJFE 100 mg/kg, (e) OA+LJFE 200 mg/kg, and (f) indomethacin 2 mg/kg. (B) The lateral subchondral bone parameters were monitored using a 3D micro-CT analysis program. The lateral subchondral bone parameters assessed were as follows: (a) bone volume (BV, mm3), (b) mean number of objects per slice (Obj.N.), and (c) cross-sectional thickness (Cs.Th, mm). The results are mean ± standard error of mean (SEM) for six rats per group (*p < 0.05, **p < 0.01 compared to NC, #p < 0.05, ##p < 0.01 compared to OAC).
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pone.0134856.g002: Three-dimensional micro-computed tomography images and parameters of lateral subchondral bone of the knee of the tibiae.(A) 3D micro-computed tomography (CT) images of the lateral subchondral bone. (a) Normal control, (b) OA control, (c) OA+70% ethanolic extract of Litsea japonica fruit (LJFE) 50 mg/kg, (d) OA+LJFE 100 mg/kg, (e) OA+LJFE 200 mg/kg, and (f) indomethacin 2 mg/kg. (B) The lateral subchondral bone parameters were monitored using a 3D micro-CT analysis program. The lateral subchondral bone parameters assessed were as follows: (a) bone volume (BV, mm3), (b) mean number of objects per slice (Obj.N.), and (c) cross-sectional thickness (Cs.Th, mm). The results are mean ± standard error of mean (SEM) for six rats per group (*p < 0.05, **p < 0.01 compared to NC, #p < 0.05, ##p < 0.01 compared to OAC).

Mentions: OA rats treated with LJFE and IM showed a lesser loss of the lateral subchondral bone than rats in the OAC group (Fig 2A). In addition the BV and Cs.Th were significantly higher, whereas the Obj.N. was significantly lower in the groups treated with LJFE and IM than in the OAC group (Fig 2B). In particular, the group receiving 200 mg/kg of LJFE for 3 weeks showed a significantly greater increase in the BV (3.1 ± 0.10 mm3, p < 0.05) and Cs.Th (0.3 ± 0.01 mm, p < 0.05) than the OAC group (2.3 ± 0.22 mm3 and 0.2 ± 0.03 mm, respectively); however, the Obj.N. (2.0 ± 0.06, p < 0.01) in this group was lower than that in the OAC group (3.0 ± 0.23) (Fig 2B).


Anti-Osteoarthritic Effects of the Litsea japonica Fruit in a Rat Model of Osteoarthritis Induced by Monosodium Iodoacetate.

Jeong YJ, Kim I, Cho JH, Park DW, Kwon JE, Jung MW, Meng X, Jo SM, Song HS, Cho YM, Song SM, Ham YM, Jung YH, Kim CS, Yoon WJ, Kang SC - PLoS ONE (2015)

Three-dimensional micro-computed tomography images and parameters of lateral subchondral bone of the knee of the tibiae.(A) 3D micro-computed tomography (CT) images of the lateral subchondral bone. (a) Normal control, (b) OA control, (c) OA+70% ethanolic extract of Litsea japonica fruit (LJFE) 50 mg/kg, (d) OA+LJFE 100 mg/kg, (e) OA+LJFE 200 mg/kg, and (f) indomethacin 2 mg/kg. (B) The lateral subchondral bone parameters were monitored using a 3D micro-CT analysis program. The lateral subchondral bone parameters assessed were as follows: (a) bone volume (BV, mm3), (b) mean number of objects per slice (Obj.N.), and (c) cross-sectional thickness (Cs.Th, mm). The results are mean ± standard error of mean (SEM) for six rats per group (*p < 0.05, **p < 0.01 compared to NC, #p < 0.05, ##p < 0.01 compared to OAC).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4526681&req=5

pone.0134856.g002: Three-dimensional micro-computed tomography images and parameters of lateral subchondral bone of the knee of the tibiae.(A) 3D micro-computed tomography (CT) images of the lateral subchondral bone. (a) Normal control, (b) OA control, (c) OA+70% ethanolic extract of Litsea japonica fruit (LJFE) 50 mg/kg, (d) OA+LJFE 100 mg/kg, (e) OA+LJFE 200 mg/kg, and (f) indomethacin 2 mg/kg. (B) The lateral subchondral bone parameters were monitored using a 3D micro-CT analysis program. The lateral subchondral bone parameters assessed were as follows: (a) bone volume (BV, mm3), (b) mean number of objects per slice (Obj.N.), and (c) cross-sectional thickness (Cs.Th, mm). The results are mean ± standard error of mean (SEM) for six rats per group (*p < 0.05, **p < 0.01 compared to NC, #p < 0.05, ##p < 0.01 compared to OAC).
Mentions: OA rats treated with LJFE and IM showed a lesser loss of the lateral subchondral bone than rats in the OAC group (Fig 2A). In addition the BV and Cs.Th were significantly higher, whereas the Obj.N. was significantly lower in the groups treated with LJFE and IM than in the OAC group (Fig 2B). In particular, the group receiving 200 mg/kg of LJFE for 3 weeks showed a significantly greater increase in the BV (3.1 ± 0.10 mm3, p < 0.05) and Cs.Th (0.3 ± 0.01 mm, p < 0.05) than the OAC group (2.3 ± 0.22 mm3 and 0.2 ± 0.03 mm, respectively); however, the Obj.N. (2.0 ± 0.06, p < 0.01) in this group was lower than that in the OAC group (3.0 ± 0.23) (Fig 2B).

Bottom Line: In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA.In addition, the LJFE decreased the expression of inflammatory cytokines.Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.

ABSTRACT
Osteoarthritis (OA) is a degenerative chronic disease that affects various tissues surrounding the joints, such as the subchondral bone and articular cartilage. The onset of OA is associated with uncontrolled catabolic and anabolic remodeling processes of the joints, including the cartilage and subchondral bone, to adapt to local biological and biochemical signals. In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA. Our results showed that administration of LJFE increased the bone volume and cross-section thickness, but the mean number of objects per slice in this group was lower than that in the OA control (OAC) group. In addition, the LJFE decreased the expression of inflammatory cytokines. Compared to the OAC group, the group treated with high doses of LJFE (100 and 200 mg/kg) showed a more than 80% inhibition of the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases. Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

No MeSH data available.


Related in: MedlinePlus