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Anti-Osteoarthritic Effects of the Litsea japonica Fruit in a Rat Model of Osteoarthritis Induced by Monosodium Iodoacetate.

Jeong YJ, Kim I, Cho JH, Park DW, Kwon JE, Jung MW, Meng X, Jo SM, Song HS, Cho YM, Song SM, Ham YM, Jung YH, Kim CS, Yoon WJ, Kang SC - PLoS ONE (2015)

Bottom Line: In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA.In addition, the LJFE decreased the expression of inflammatory cytokines.Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.

ABSTRACT
Osteoarthritis (OA) is a degenerative chronic disease that affects various tissues surrounding the joints, such as the subchondral bone and articular cartilage. The onset of OA is associated with uncontrolled catabolic and anabolic remodeling processes of the joints, including the cartilage and subchondral bone, to adapt to local biological and biochemical signals. In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA. Our results showed that administration of LJFE increased the bone volume and cross-section thickness, but the mean number of objects per slice in this group was lower than that in the OA control (OAC) group. In addition, the LJFE decreased the expression of inflammatory cytokines. Compared to the OAC group, the group treated with high doses of LJFE (100 and 200 mg/kg) showed a more than 80% inhibition of the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases. Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

No MeSH data available.


Related in: MedlinePlus

HPLC chromatogram of the 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE).HPLC was performed using a Symmetry C18 column, 5 μL injection at 30°C, and detection at 254 nm.
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pone.0134856.g001: HPLC chromatogram of the 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE).HPLC was performed using a Symmetry C18 column, 5 μL injection at 30°C, and detection at 254 nm.

Mentions: In previous studies, we separated the active ingredients of LJFE hamabiwalactone A (3-[(1E)-1-dodecen-11-yn-1yl]-5-methyl-2(5H)-furanone, C17H26O2) and hamabiwalactone B (2(5H)-furanone,3-(1E)-1,11-dodecadien-1-yl-5-methyl-, (5S)-, C17H26O2), which have anti-inflammatory and analgesic effects [16]. OA is characterized by joint pain, and inflammation is substantially involved in the pathogenesis and progression of OA [1,8]. Therefore, hamabiwalactone A and B present in the L. japonica fruit may be used as active ingredients for the treatment of OA, and in this study, we measured the levels of hamabiwalactone A and B in the LJFE. Our results showed that content of hamabiwalactone A in the LFJE was 12.1 ± 0.08 mg/g and that of hamabiwalactone B was 15.9 ± 0.09 mg/g (Fig 1).


Anti-Osteoarthritic Effects of the Litsea japonica Fruit in a Rat Model of Osteoarthritis Induced by Monosodium Iodoacetate.

Jeong YJ, Kim I, Cho JH, Park DW, Kwon JE, Jung MW, Meng X, Jo SM, Song HS, Cho YM, Song SM, Ham YM, Jung YH, Kim CS, Yoon WJ, Kang SC - PLoS ONE (2015)

HPLC chromatogram of the 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE).HPLC was performed using a Symmetry C18 column, 5 μL injection at 30°C, and detection at 254 nm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526681&req=5

pone.0134856.g001: HPLC chromatogram of the 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE).HPLC was performed using a Symmetry C18 column, 5 μL injection at 30°C, and detection at 254 nm.
Mentions: In previous studies, we separated the active ingredients of LJFE hamabiwalactone A (3-[(1E)-1-dodecen-11-yn-1yl]-5-methyl-2(5H)-furanone, C17H26O2) and hamabiwalactone B (2(5H)-furanone,3-(1E)-1,11-dodecadien-1-yl-5-methyl-, (5S)-, C17H26O2), which have anti-inflammatory and analgesic effects [16]. OA is characterized by joint pain, and inflammation is substantially involved in the pathogenesis and progression of OA [1,8]. Therefore, hamabiwalactone A and B present in the L. japonica fruit may be used as active ingredients for the treatment of OA, and in this study, we measured the levels of hamabiwalactone A and B in the LJFE. Our results showed that content of hamabiwalactone A in the LFJE was 12.1 ± 0.08 mg/g and that of hamabiwalactone B was 15.9 ± 0.09 mg/g (Fig 1).

Bottom Line: In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA.In addition, the LJFE decreased the expression of inflammatory cytokines.Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Gachon University, Seongnam, Republic of Korea.

ABSTRACT
Osteoarthritis (OA) is a degenerative chronic disease that affects various tissues surrounding the joints, such as the subchondral bone and articular cartilage. The onset of OA is associated with uncontrolled catabolic and anabolic remodeling processes of the joints, including the cartilage and subchondral bone, to adapt to local biological and biochemical signals. In this study, we determined whether 70% ethanolic (EtOH) extract of Litsea japonica fruit (LJFE) had beneficial effects on the articular cartilage, including structural changes in the tibial subchondral bone, matrix degradation, and inflammatory responses, in OA by using a rat model of monosodium iodoacetate-induced OA. Our results showed that administration of LJFE increased the bone volume and cross-section thickness, but the mean number of objects per slice in this group was lower than that in the OA control (OAC) group. In addition, the LJFE decreased the expression of inflammatory cytokines. Compared to the OAC group, the group treated with high doses of LJFE (100 and 200 mg/kg) showed a more than 80% inhibition of the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases. Our results suggest that LJFE can be used as a potential anti-osteoarthritic agent.

No MeSH data available.


Related in: MedlinePlus