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High Glucose Concentration Promotes Vancomycin-Enhanced Biofilm Formation of Vancomycin-Non-Susceptible Staphylococcus aureus in Diabetic Mice.

Hsu CY, Shu JC, Lin MH, Chong KY, Chen CC, Wen SM, Hsieh YT, Liao WT - PLoS ONE (2015)

Bottom Line: To address this question, the diabetic mouse model infected by vancomycin-resistant S. aureus (VRSA) was used under vancomycin treatment.A 10- and 1000-fold increase in biofilm-bound bacterial colony forming units was observed in samples from diabetic mice without and with vancomycin treatment, respectively, compared to healthy mice.By contrast, in the absence of glucose vancomycin reduced propensity to form biofilms in vitro through the increased production of proteases and DNases from VRSA.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, No. 259, Wenhua 1st Road, Guishan, Taoyuan 333, Taiwan.

ABSTRACT
We previously demonstrated that vancomycin treatment increased acquisition of eDNA and enhanced biofilm formation of drug-resistant Staphylococcus aureus through a cidA-mediated autolysis mechanism. Recently we found that such enhancement became more significant under a higher glucose concentration in vitro. We propose that besides improper antibiotic treatment, increased glucose concentration environment in diabetic animals may further enhance biofilm formation of drug-resistant S. aureus. To address this question, the diabetic mouse model infected by vancomycin-resistant S. aureus (VRSA) was used under vancomycin treatment. The capacity to form biofilms was evaluated through a catheter-associated biofilm assay. A 10- and 1000-fold increase in biofilm-bound bacterial colony forming units was observed in samples from diabetic mice without and with vancomycin treatment, respectively, compared to healthy mice. By contrast, in the absence of glucose vancomycin reduced propensity to form biofilms in vitro through the increased production of proteases and DNases from VRSA. Our study highlights the potentially important role of increased glucose concentration in enhancing biofilm formation in vancomycin-treated diabetic mice infected by drug-resistant S. aureus.

No MeSH data available.


Related in: MedlinePlus

Vancomycin plays a different role in bladder catheter biofilm formation between healthy and diabetic rats.Determination of the number of viable biofilm-bound bacteria in the bladder catheters removed from healthy/diabetic rats infected with VRSA SJC1200 in the absence/presence of vancomycin treatment. Six rats were used in each group.
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pone.0134852.g004: Vancomycin plays a different role in bladder catheter biofilm formation between healthy and diabetic rats.Determination of the number of viable biofilm-bound bacteria in the bladder catheters removed from healthy/diabetic rats infected with VRSA SJC1200 in the absence/presence of vancomycin treatment. Six rats were used in each group.

Mentions: As shown in Fig 1, vancomycin reduced the propensity to form biofilms in the absence of glucose, and the urine glucose concentration is always undetectable in healthy people and rats. We therefore investigated the effect of vancomycin treatment on catheter-associated biofilm formation in a urinary bladder following the same treatment protocol as above. Mice were replaced by rats because of the enough bladder space for the implantation of catheters. Both the inner and outer walls of the catheter were covered by biofilm materials when the catheters were removed from the bladders. Among the healthy rats infected by VRSA, a significant decrease in biofilm formation was found in vancomycin-treated rats compared to untreated rats (P < 0.05). The biofilm-forming capacity is approximately 10 times higher in diabetic rats than that in healthy rats when vancomycin was not applied (P < 0.001). A further 10-fold increase in biofilm formation was observed in diabetic rats upon vancomycin treatment (P < 0.05; Fig 4). Above results suggested that glucose was essential for the vancomycin-enhanced biofilm formation.


High Glucose Concentration Promotes Vancomycin-Enhanced Biofilm Formation of Vancomycin-Non-Susceptible Staphylococcus aureus in Diabetic Mice.

Hsu CY, Shu JC, Lin MH, Chong KY, Chen CC, Wen SM, Hsieh YT, Liao WT - PLoS ONE (2015)

Vancomycin plays a different role in bladder catheter biofilm formation between healthy and diabetic rats.Determination of the number of viable biofilm-bound bacteria in the bladder catheters removed from healthy/diabetic rats infected with VRSA SJC1200 in the absence/presence of vancomycin treatment. Six rats were used in each group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526670&req=5

pone.0134852.g004: Vancomycin plays a different role in bladder catheter biofilm formation between healthy and diabetic rats.Determination of the number of viable biofilm-bound bacteria in the bladder catheters removed from healthy/diabetic rats infected with VRSA SJC1200 in the absence/presence of vancomycin treatment. Six rats were used in each group.
Mentions: As shown in Fig 1, vancomycin reduced the propensity to form biofilms in the absence of glucose, and the urine glucose concentration is always undetectable in healthy people and rats. We therefore investigated the effect of vancomycin treatment on catheter-associated biofilm formation in a urinary bladder following the same treatment protocol as above. Mice were replaced by rats because of the enough bladder space for the implantation of catheters. Both the inner and outer walls of the catheter were covered by biofilm materials when the catheters were removed from the bladders. Among the healthy rats infected by VRSA, a significant decrease in biofilm formation was found in vancomycin-treated rats compared to untreated rats (P < 0.05). The biofilm-forming capacity is approximately 10 times higher in diabetic rats than that in healthy rats when vancomycin was not applied (P < 0.001). A further 10-fold increase in biofilm formation was observed in diabetic rats upon vancomycin treatment (P < 0.05; Fig 4). Above results suggested that glucose was essential for the vancomycin-enhanced biofilm formation.

Bottom Line: To address this question, the diabetic mouse model infected by vancomycin-resistant S. aureus (VRSA) was used under vancomycin treatment.A 10- and 1000-fold increase in biofilm-bound bacterial colony forming units was observed in samples from diabetic mice without and with vancomycin treatment, respectively, compared to healthy mice.By contrast, in the absence of glucose vancomycin reduced propensity to form biofilms in vitro through the increased production of proteases and DNases from VRSA.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, No. 259, Wenhua 1st Road, Guishan, Taoyuan 333, Taiwan.

ABSTRACT
We previously demonstrated that vancomycin treatment increased acquisition of eDNA and enhanced biofilm formation of drug-resistant Staphylococcus aureus through a cidA-mediated autolysis mechanism. Recently we found that such enhancement became more significant under a higher glucose concentration in vitro. We propose that besides improper antibiotic treatment, increased glucose concentration environment in diabetic animals may further enhance biofilm formation of drug-resistant S. aureus. To address this question, the diabetic mouse model infected by vancomycin-resistant S. aureus (VRSA) was used under vancomycin treatment. The capacity to form biofilms was evaluated through a catheter-associated biofilm assay. A 10- and 1000-fold increase in biofilm-bound bacterial colony forming units was observed in samples from diabetic mice without and with vancomycin treatment, respectively, compared to healthy mice. By contrast, in the absence of glucose vancomycin reduced propensity to form biofilms in vitro through the increased production of proteases and DNases from VRSA. Our study highlights the potentially important role of increased glucose concentration in enhancing biofilm formation in vancomycin-treated diabetic mice infected by drug-resistant S. aureus.

No MeSH data available.


Related in: MedlinePlus