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Thuniopsis: A New Orchid Genus and Phylogeny of the Tribe Arethuseae (Orchidaceae).

Li L, Ye DP, Niu M, Yan HF, Wen TL, Li SJ - PLoS ONE (2015)

Bottom Line: The new genus is circumscribed and a description and illustrations of the new species are provided.The phylogenetic relationships among the genera in Arethuseae are accessed.Moreover, our phylogeny also shed light on the phylogenetic positions of several genera which, to date, remain uncertain.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, P. R. China.

ABSTRACT
An investigation of a questionable orchid led to the discovery of a new genus and species Thuniopsis cleistogama, endemic to Yunnan province, China. It is characterized by having a subglobose corm, a spike-like (racemose) inflorescence, half opened and spurless flowers, a collar-shaped stigma and subglobose capsules. Based on DNA sequence data from three gene regions (nuclear ribosomal ITS, chloroplast matK and trnL), we investigated its phylogenetic position within the tribe Arethuseae. Phylogenies using maximum likelihood and Bayesian inference support the recognition of Thuniopsis as a distinct genus, and suggest its close relationship to the genera Bletilla, Dilochia, and Thunia. The new genus is circumscribed and a description and illustrations of the new species are provided. The phylogenetic relationships among the genera in Arethuseae are accessed. Moreover, our phylogeny also shed light on the phylogenetic positions of several genera which, to date, remain uncertain.

No MeSH data available.


Related in: MedlinePlus

The phylogeny of Arethuseae produced by GARLI and MrBayes using sequence data from nrDNA ITS and cpDNA matK datasets.Maximum likelihood bootstrap values and Bayesian posterior probabilities are shown above and below branches, respectively. Bold letters signify clades that are discussed in further detail in the text. The phylogenetic position of Thuniopsis cleistogama is highlighted. Asterisk (*) indicate branches resolved only by the BI analysis.
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pone.0132777.g001: The phylogeny of Arethuseae produced by GARLI and MrBayes using sequence data from nrDNA ITS and cpDNA matK datasets.Maximum likelihood bootstrap values and Bayesian posterior probabilities are shown above and below branches, respectively. Bold letters signify clades that are discussed in further detail in the text. The phylogenetic position of Thuniopsis cleistogama is highlighted. Asterisk (*) indicate branches resolved only by the BI analysis.

Mentions: The ML analyses recovered relationships similar to those inferred by BI analyses, differing only in poorly supported internal branches. In some cases, the posterior probabilities are higher than the ML bootstrap percentages, but all clades with high posterior probabilities also receive at least moderate bootstrap support in the ML analyses. The ML phylogeny tree obtained from all three DNA regions by using all ingroup taxa (including those that missing trnL) is shown in S3 Fig. The trees generated from all data matrices, both separate and combined, had essentially the same topology. The topology of the combined ITS and matK is largely consistent with that recovered in the analysis of the combined dataset of all three DNA regions, but with somewhat higher clade resolution within Thunia and its related genera, which was selected to represent the evolutionary relationships within the tribe, with PP from the BI analysis shown where applicable (Fig 1). This analysis included 47 samples that had sequence data from both ITS and matK regions, 61 samples that had ITS sequence data, 50 samples that had matK sequence data (S2 Table).


Thuniopsis: A New Orchid Genus and Phylogeny of the Tribe Arethuseae (Orchidaceae).

Li L, Ye DP, Niu M, Yan HF, Wen TL, Li SJ - PLoS ONE (2015)

The phylogeny of Arethuseae produced by GARLI and MrBayes using sequence data from nrDNA ITS and cpDNA matK datasets.Maximum likelihood bootstrap values and Bayesian posterior probabilities are shown above and below branches, respectively. Bold letters signify clades that are discussed in further detail in the text. The phylogenetic position of Thuniopsis cleistogama is highlighted. Asterisk (*) indicate branches resolved only by the BI analysis.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526666&req=5

pone.0132777.g001: The phylogeny of Arethuseae produced by GARLI and MrBayes using sequence data from nrDNA ITS and cpDNA matK datasets.Maximum likelihood bootstrap values and Bayesian posterior probabilities are shown above and below branches, respectively. Bold letters signify clades that are discussed in further detail in the text. The phylogenetic position of Thuniopsis cleistogama is highlighted. Asterisk (*) indicate branches resolved only by the BI analysis.
Mentions: The ML analyses recovered relationships similar to those inferred by BI analyses, differing only in poorly supported internal branches. In some cases, the posterior probabilities are higher than the ML bootstrap percentages, but all clades with high posterior probabilities also receive at least moderate bootstrap support in the ML analyses. The ML phylogeny tree obtained from all three DNA regions by using all ingroup taxa (including those that missing trnL) is shown in S3 Fig. The trees generated from all data matrices, both separate and combined, had essentially the same topology. The topology of the combined ITS and matK is largely consistent with that recovered in the analysis of the combined dataset of all three DNA regions, but with somewhat higher clade resolution within Thunia and its related genera, which was selected to represent the evolutionary relationships within the tribe, with PP from the BI analysis shown where applicable (Fig 1). This analysis included 47 samples that had sequence data from both ITS and matK regions, 61 samples that had ITS sequence data, 50 samples that had matK sequence data (S2 Table).

Bottom Line: The new genus is circumscribed and a description and illustrations of the new species are provided.The phylogenetic relationships among the genera in Arethuseae are accessed.Moreover, our phylogeny also shed light on the phylogenetic positions of several genera which, to date, remain uncertain.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, P. R. China.

ABSTRACT
An investigation of a questionable orchid led to the discovery of a new genus and species Thuniopsis cleistogama, endemic to Yunnan province, China. It is characterized by having a subglobose corm, a spike-like (racemose) inflorescence, half opened and spurless flowers, a collar-shaped stigma and subglobose capsules. Based on DNA sequence data from three gene regions (nuclear ribosomal ITS, chloroplast matK and trnL), we investigated its phylogenetic position within the tribe Arethuseae. Phylogenies using maximum likelihood and Bayesian inference support the recognition of Thuniopsis as a distinct genus, and suggest its close relationship to the genera Bletilla, Dilochia, and Thunia. The new genus is circumscribed and a description and illustrations of the new species are provided. The phylogenetic relationships among the genera in Arethuseae are accessed. Moreover, our phylogeny also shed light on the phylogenetic positions of several genera which, to date, remain uncertain.

No MeSH data available.


Related in: MedlinePlus