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Hippocampal Synaptic Expansion Induced by Spatial Experience in Rats Correlates with Improved Information Processing in the Hippocampus.

Carasatorre M, Ochoa-Alvarez A, Velázquez-Campos G, Lozano-Flores C, Ramírez-Amaya V, Díaz-Cintra SY - PLoS ONE (2015)

Bottom Line: The "catFISH" imaging method provided neurophysiological evidence that hippocampal pattern separation improved in animals treated as SC, and this improvement was even clearer in animals that experienced the WM training.By measuring the area occupied by synaptophysin staining in both the stratum oriens and the stratun lucidum of the distal CA3, we found evidence of structural synaptic plasticity that likely includes MF expansion.Finally, the measures of hippocampal network coding obtained with catFISH correlate significantly with the increased density of synaptophysin staining, strongly suggesting that structural synaptic plasticity in the hippocampus induced by the WM and SC experience is related to the improvement of spatial information processing in the hippocampus.

View Article: PubMed Central - PubMed

Affiliation: Department of "Neurobiología del Desarrollo y Neurofisiología, Instituto de Neurobiología", Universidad Nacional Autónoma de México, Querétaro, México.

ABSTRACT
Spatial water maze (WM) overtraining induces hippocampal mossy fiber (MF) expansion, and it has been suggested that spatial pattern separation depends on the MF pathway. We hypothesized that WM experience inducing MF expansion in rats would improve spatial pattern separation in the hippocampal network. We first tested this by using the the delayed non-matching to place task (DNMP), in animals that had been previously trained on the water maze (WM) and found that these animals, as well as animals treated as swim controls (SC), performed better than home cage control animals the DNMP task. The "catFISH" imaging method provided neurophysiological evidence that hippocampal pattern separation improved in animals treated as SC, and this improvement was even clearer in animals that experienced the WM training. Moreover, these behavioral treatments also enhance network reliability and improve partial pattern separation in CA1 and pattern completion in CA3. By measuring the area occupied by synaptophysin staining in both the stratum oriens and the stratun lucidum of the distal CA3, we found evidence of structural synaptic plasticity that likely includes MF expansion. Finally, the measures of hippocampal network coding obtained with catFISH correlate significantly with the increased density of synaptophysin staining, strongly suggesting that structural synaptic plasticity in the hippocampus induced by the WM and SC experience is related to the improvement of spatial information processing in the hippocampus.

No MeSH data available.


Related in: MedlinePlus

Synaptophysin/Map2 area and Similarity score correlation analysis.Correlation graphs between: the area of synaptophysin staining in the CA3 SOd and the similarity scores (SiSc) obtained in the CA3 network (first row); the area of synaptophysin staining in the CA3 SOd and the SiSc obtained in the CA1 network (second row); the area of synaptophysin staining in the CA3 SLd and the SiSc obtained in the CA3 network (Third row); and the area of synaptophysin staining in the CA3 SLd and the SiSc obtained in the CA1 network (Fourth row). The first column presents the correlation graphs for the AA double exploration condition, where pattern completion is observed in both the CA3 and CA1 networks; the second column presents the correlation graphs for the AA’ condition, where pattern completion occurred in the CA3 network, and partial pattern separation occurred in CA1; and the third column presents the graphs for the AB condition where pattern separation occurred in both CA1 and CA3. ***p<0.001, **p<0.01, *p<0.05.
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pone.0132676.g009: Synaptophysin/Map2 area and Similarity score correlation analysis.Correlation graphs between: the area of synaptophysin staining in the CA3 SOd and the similarity scores (SiSc) obtained in the CA3 network (first row); the area of synaptophysin staining in the CA3 SOd and the SiSc obtained in the CA1 network (second row); the area of synaptophysin staining in the CA3 SLd and the SiSc obtained in the CA3 network (Third row); and the area of synaptophysin staining in the CA3 SLd and the SiSc obtained in the CA1 network (Fourth row). The first column presents the correlation graphs for the AA double exploration condition, where pattern completion is observed in both the CA3 and CA1 networks; the second column presents the correlation graphs for the AA’ condition, where pattern completion occurred in the CA3 network, and partial pattern separation occurred in CA1; and the third column presents the graphs for the AB condition where pattern separation occurred in both CA1 and CA3. ***p<0.001, **p<0.01, *p<0.05.

Mentions: The SiSc obtained in the CA3 network showed significant, positive correlations with the synaptophysin-stained area in the CA3-SOd (Fig 9; S6 File) after both the AA (r (40) = 0.907 p<0.001) and AA’ double exploration (r (40) = 0.870 p<0.001), indicating that what may be primarily MF in the CA3-SOd correlates with a higher neural ensemble overlap in CA3, which suggests a more reliable network coding in CA3 and improved proper pattern completion.


Hippocampal Synaptic Expansion Induced by Spatial Experience in Rats Correlates with Improved Information Processing in the Hippocampus.

Carasatorre M, Ochoa-Alvarez A, Velázquez-Campos G, Lozano-Flores C, Ramírez-Amaya V, Díaz-Cintra SY - PLoS ONE (2015)

Synaptophysin/Map2 area and Similarity score correlation analysis.Correlation graphs between: the area of synaptophysin staining in the CA3 SOd and the similarity scores (SiSc) obtained in the CA3 network (first row); the area of synaptophysin staining in the CA3 SOd and the SiSc obtained in the CA1 network (second row); the area of synaptophysin staining in the CA3 SLd and the SiSc obtained in the CA3 network (Third row); and the area of synaptophysin staining in the CA3 SLd and the SiSc obtained in the CA1 network (Fourth row). The first column presents the correlation graphs for the AA double exploration condition, where pattern completion is observed in both the CA3 and CA1 networks; the second column presents the correlation graphs for the AA’ condition, where pattern completion occurred in the CA3 network, and partial pattern separation occurred in CA1; and the third column presents the graphs for the AB condition where pattern separation occurred in both CA1 and CA3. ***p<0.001, **p<0.01, *p<0.05.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4526663&req=5

pone.0132676.g009: Synaptophysin/Map2 area and Similarity score correlation analysis.Correlation graphs between: the area of synaptophysin staining in the CA3 SOd and the similarity scores (SiSc) obtained in the CA3 network (first row); the area of synaptophysin staining in the CA3 SOd and the SiSc obtained in the CA1 network (second row); the area of synaptophysin staining in the CA3 SLd and the SiSc obtained in the CA3 network (Third row); and the area of synaptophysin staining in the CA3 SLd and the SiSc obtained in the CA1 network (Fourth row). The first column presents the correlation graphs for the AA double exploration condition, where pattern completion is observed in both the CA3 and CA1 networks; the second column presents the correlation graphs for the AA’ condition, where pattern completion occurred in the CA3 network, and partial pattern separation occurred in CA1; and the third column presents the graphs for the AB condition where pattern separation occurred in both CA1 and CA3. ***p<0.001, **p<0.01, *p<0.05.
Mentions: The SiSc obtained in the CA3 network showed significant, positive correlations with the synaptophysin-stained area in the CA3-SOd (Fig 9; S6 File) after both the AA (r (40) = 0.907 p<0.001) and AA’ double exploration (r (40) = 0.870 p<0.001), indicating that what may be primarily MF in the CA3-SOd correlates with a higher neural ensemble overlap in CA3, which suggests a more reliable network coding in CA3 and improved proper pattern completion.

Bottom Line: The "catFISH" imaging method provided neurophysiological evidence that hippocampal pattern separation improved in animals treated as SC, and this improvement was even clearer in animals that experienced the WM training.By measuring the area occupied by synaptophysin staining in both the stratum oriens and the stratun lucidum of the distal CA3, we found evidence of structural synaptic plasticity that likely includes MF expansion.Finally, the measures of hippocampal network coding obtained with catFISH correlate significantly with the increased density of synaptophysin staining, strongly suggesting that structural synaptic plasticity in the hippocampus induced by the WM and SC experience is related to the improvement of spatial information processing in the hippocampus.

View Article: PubMed Central - PubMed

Affiliation: Department of "Neurobiología del Desarrollo y Neurofisiología, Instituto de Neurobiología", Universidad Nacional Autónoma de México, Querétaro, México.

ABSTRACT
Spatial water maze (WM) overtraining induces hippocampal mossy fiber (MF) expansion, and it has been suggested that spatial pattern separation depends on the MF pathway. We hypothesized that WM experience inducing MF expansion in rats would improve spatial pattern separation in the hippocampal network. We first tested this by using the the delayed non-matching to place task (DNMP), in animals that had been previously trained on the water maze (WM) and found that these animals, as well as animals treated as swim controls (SC), performed better than home cage control animals the DNMP task. The "catFISH" imaging method provided neurophysiological evidence that hippocampal pattern separation improved in animals treated as SC, and this improvement was even clearer in animals that experienced the WM training. Moreover, these behavioral treatments also enhance network reliability and improve partial pattern separation in CA1 and pattern completion in CA3. By measuring the area occupied by synaptophysin staining in both the stratum oriens and the stratun lucidum of the distal CA3, we found evidence of structural synaptic plasticity that likely includes MF expansion. Finally, the measures of hippocampal network coding obtained with catFISH correlate significantly with the increased density of synaptophysin staining, strongly suggesting that structural synaptic plasticity in the hippocampus induced by the WM and SC experience is related to the improvement of spatial information processing in the hippocampus.

No MeSH data available.


Related in: MedlinePlus