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Hippocampal Synaptic Expansion Induced by Spatial Experience in Rats Correlates with Improved Information Processing in the Hippocampus.

Carasatorre M, Ochoa-Alvarez A, Velázquez-Campos G, Lozano-Flores C, Ramírez-Amaya V, Díaz-Cintra SY - PLoS ONE (2015)

Bottom Line: The "catFISH" imaging method provided neurophysiological evidence that hippocampal pattern separation improved in animals treated as SC, and this improvement was even clearer in animals that experienced the WM training.By measuring the area occupied by synaptophysin staining in both the stratum oriens and the stratun lucidum of the distal CA3, we found evidence of structural synaptic plasticity that likely includes MF expansion.Finally, the measures of hippocampal network coding obtained with catFISH correlate significantly with the increased density of synaptophysin staining, strongly suggesting that structural synaptic plasticity in the hippocampus induced by the WM and SC experience is related to the improvement of spatial information processing in the hippocampus.

View Article: PubMed Central - PubMed

Affiliation: Department of "Neurobiología del Desarrollo y Neurofisiología, Instituto de Neurobiología", Universidad Nacional Autónoma de México, Querétaro, México.

ABSTRACT
Spatial water maze (WM) overtraining induces hippocampal mossy fiber (MF) expansion, and it has been suggested that spatial pattern separation depends on the MF pathway. We hypothesized that WM experience inducing MF expansion in rats would improve spatial pattern separation in the hippocampal network. We first tested this by using the the delayed non-matching to place task (DNMP), in animals that had been previously trained on the water maze (WM) and found that these animals, as well as animals treated as swim controls (SC), performed better than home cage control animals the DNMP task. The "catFISH" imaging method provided neurophysiological evidence that hippocampal pattern separation improved in animals treated as SC, and this improvement was even clearer in animals that experienced the WM training. Moreover, these behavioral treatments also enhance network reliability and improve partial pattern separation in CA1 and pattern completion in CA3. By measuring the area occupied by synaptophysin staining in both the stratum oriens and the stratun lucidum of the distal CA3, we found evidence of structural synaptic plasticity that likely includes MF expansion. Finally, the measures of hippocampal network coding obtained with catFISH correlate significantly with the increased density of synaptophysin staining, strongly suggesting that structural synaptic plasticity in the hippocampus induced by the WM and SC experience is related to the improvement of spatial information processing in the hippocampus.

No MeSH data available.


Related in: MedlinePlus

The similarity score (SiSc) results from catFISH.This measure takes into account the 3 Arc staining classifications and expresses it in a single value. This value (±SEM) represents the degree of overlap between the two recruited ensemble in the CA1 (A) and CA3 (B) networks from animals exposed to the AA, AA’, and AB double exploration conditions after being pre-treated as either intact controls (IC), swimming controls (SC), or water maze trained (WM). Bonferroni post hoc analysis of the WM group vs. its respective IC ***p<0.001, **p<0.01, *p<0.05; or vs. its respective SC (###p<0.001, #p<0.05), and the important intragroup differences are shown with lines (***p<0.001, **p<0.01).
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pone.0132676.g005: The similarity score (SiSc) results from catFISH.This measure takes into account the 3 Arc staining classifications and expresses it in a single value. This value (±SEM) represents the degree of overlap between the two recruited ensemble in the CA1 (A) and CA3 (B) networks from animals exposed to the AA, AA’, and AB double exploration conditions after being pre-treated as either intact controls (IC), swimming controls (SC), or water maze trained (WM). Bonferroni post hoc analysis of the WM group vs. its respective IC ***p<0.001, **p<0.01, *p<0.05; or vs. its respective SC (###p<0.001, #p<0.05), and the important intragroup differences are shown with lines (***p<0.001, **p<0.01).

Mentions: A value close to 1 represents a single neuronal population faithfully activated in both behavioral epochs, whereas a value close to 0 indicates that two, statistically independent cell populations were activated during the two epochs [Fig 5].


Hippocampal Synaptic Expansion Induced by Spatial Experience in Rats Correlates with Improved Information Processing in the Hippocampus.

Carasatorre M, Ochoa-Alvarez A, Velázquez-Campos G, Lozano-Flores C, Ramírez-Amaya V, Díaz-Cintra SY - PLoS ONE (2015)

The similarity score (SiSc) results from catFISH.This measure takes into account the 3 Arc staining classifications and expresses it in a single value. This value (±SEM) represents the degree of overlap between the two recruited ensemble in the CA1 (A) and CA3 (B) networks from animals exposed to the AA, AA’, and AB double exploration conditions after being pre-treated as either intact controls (IC), swimming controls (SC), or water maze trained (WM). Bonferroni post hoc analysis of the WM group vs. its respective IC ***p<0.001, **p<0.01, *p<0.05; or vs. its respective SC (###p<0.001, #p<0.05), and the important intragroup differences are shown with lines (***p<0.001, **p<0.01).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526663&req=5

pone.0132676.g005: The similarity score (SiSc) results from catFISH.This measure takes into account the 3 Arc staining classifications and expresses it in a single value. This value (±SEM) represents the degree of overlap between the two recruited ensemble in the CA1 (A) and CA3 (B) networks from animals exposed to the AA, AA’, and AB double exploration conditions after being pre-treated as either intact controls (IC), swimming controls (SC), or water maze trained (WM). Bonferroni post hoc analysis of the WM group vs. its respective IC ***p<0.001, **p<0.01, *p<0.05; or vs. its respective SC (###p<0.001, #p<0.05), and the important intragroup differences are shown with lines (***p<0.001, **p<0.01).
Mentions: A value close to 1 represents a single neuronal population faithfully activated in both behavioral epochs, whereas a value close to 0 indicates that two, statistically independent cell populations were activated during the two epochs [Fig 5].

Bottom Line: The "catFISH" imaging method provided neurophysiological evidence that hippocampal pattern separation improved in animals treated as SC, and this improvement was even clearer in animals that experienced the WM training.By measuring the area occupied by synaptophysin staining in both the stratum oriens and the stratun lucidum of the distal CA3, we found evidence of structural synaptic plasticity that likely includes MF expansion.Finally, the measures of hippocampal network coding obtained with catFISH correlate significantly with the increased density of synaptophysin staining, strongly suggesting that structural synaptic plasticity in the hippocampus induced by the WM and SC experience is related to the improvement of spatial information processing in the hippocampus.

View Article: PubMed Central - PubMed

Affiliation: Department of "Neurobiología del Desarrollo y Neurofisiología, Instituto de Neurobiología", Universidad Nacional Autónoma de México, Querétaro, México.

ABSTRACT
Spatial water maze (WM) overtraining induces hippocampal mossy fiber (MF) expansion, and it has been suggested that spatial pattern separation depends on the MF pathway. We hypothesized that WM experience inducing MF expansion in rats would improve spatial pattern separation in the hippocampal network. We first tested this by using the the delayed non-matching to place task (DNMP), in animals that had been previously trained on the water maze (WM) and found that these animals, as well as animals treated as swim controls (SC), performed better than home cage control animals the DNMP task. The "catFISH" imaging method provided neurophysiological evidence that hippocampal pattern separation improved in animals treated as SC, and this improvement was even clearer in animals that experienced the WM training. Moreover, these behavioral treatments also enhance network reliability and improve partial pattern separation in CA1 and pattern completion in CA3. By measuring the area occupied by synaptophysin staining in both the stratum oriens and the stratun lucidum of the distal CA3, we found evidence of structural synaptic plasticity that likely includes MF expansion. Finally, the measures of hippocampal network coding obtained with catFISH correlate significantly with the increased density of synaptophysin staining, strongly suggesting that structural synaptic plasticity in the hippocampus induced by the WM and SC experience is related to the improvement of spatial information processing in the hippocampus.

No MeSH data available.


Related in: MedlinePlus