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α-Tocopherol Improves Microcirculatory Dysfunction on Fructose Fed Hamsters.

Boa BC, Barros CM, Souza Md, Castiglione RC, Cyrino FZ, Bouskela E - PLoS ONE (2015)

Bottom Line: On the other hand, vitamin E supplementation resulted in increased responses for both water and fructose drinking groups (177.4% for F vs.The number of leaky sites after I/R and histamine stimuli in vitamin E supplemented animals decreased (-25.1% and -15.3% for F vs.Our results strongly suggest that vitamin E could improve the endothelial function and permeability barrier and also reverse impairments elicited by sugar overload.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Clinical and Experimental Research on Vascular Biology (BioVasc), Biomedical Center, State University of Rio de Janeiro, Rio de Janeiro, Brazil.

ABSTRACT
Fructose, an everyday component of western diet associated to chronic hyperglycemia and enhanced free radical production, impairs endothelial function and supplementation with antioxidants might improve it. In this study we investigated if vitamin E could reverse the microvascular damage elicited by fructose. Male Syrian golden hamsters drank either 10% fructose solution (F) or filtered water (C), combined with three concentrations of vitamin E in their chows [zero, normal (VE) or 5X (5XVE)] during 60 days. Microvascular reactivity in response to topical application of acetylcholine (Ach; endothelium-dependent vasodilator) or sodium nitroprusside (SNP; endothelium-independent vasodilator) and macromolecular permeability increase induced by either 30 min ischemia followed by reperfusion (I/R) or topical application of histamine (5 μM) were assessed using the cheek pouch preparation. Compared to controls (drinking filtered water), fructose-drinking animals showed decreased vasodilatation to acetylcholine in all concentrations tested (-56.2% for 10-9M, -53.9% for 10-7M and -43.7% for 10-5M). On the other hand, vitamin E supplementation resulted in increased responses for both water and fructose drinking groups (177.4% for F vs. F/5XVE and 241.6% for C vs. C/5XVE for 10-5M Ach). Endothelial-independent vasodilatation explored by topical application of SNP was restored and even enhanced with the supplementation of 5X vitamin E in both groups (80.1% for F vs. F/5XVE; 144.2% for C vs. C/5XVE; 3.4% of difference for C/5XVE vs. F/5XVE on 10-5M SNP). The number of leaky sites after I/R and histamine stimuli in vitamin E supplemented animals decreased (-25.1% and -15.3% for F vs. F/5XVE; and -21.7% and -16% of leaky sites comparing C vs. C/5XVE, respectively for I/R and histamine stimuli) pointing to tightening of the endothelial barrier for macromolecular permeability. Our results strongly suggest that vitamin E could improve the endothelial function and permeability barrier and also reverse impairments elicited by sugar overload.

No MeSH data available.


Related in: MedlinePlus

Microvascular permeability measurements after I/R procedure and topical application of histamine.Data are shown as number of leaks, expressed as mean ± SD and plotted in column bars. (A) Number of extravasations after 30 min ischemia followed by reperfusion, 10 minutes after the onset of reperfusion. Significant differences were found between *C vs. F (p<0.01), + C vs. C/VE and C/5XVE (p<0.001), and F vs. F/VE and F/5XVE (p<0.01). (B) Histamine-mediated microvascular permeability. Animals treated with 10% fructose solution without vitamin E in the chow presented higher number of leaky sites, when compared to controls (C—filtered water without vitamin E in the chow) (*p<0.01- F vs. C). Supplementation with vitamin E restored microcirculatory function, as improvements may be seen on both fructose (#p<0.01 –F vs. F/5XVE) and control responses (+p<0.01 –C vs. C/5XVE).
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pone.0134740.g004: Microvascular permeability measurements after I/R procedure and topical application of histamine.Data are shown as number of leaks, expressed as mean ± SD and plotted in column bars. (A) Number of extravasations after 30 min ischemia followed by reperfusion, 10 minutes after the onset of reperfusion. Significant differences were found between *C vs. F (p<0.01), + C vs. C/VE and C/5XVE (p<0.001), and F vs. F/VE and F/5XVE (p<0.01). (B) Histamine-mediated microvascular permeability. Animals treated with 10% fructose solution without vitamin E in the chow presented higher number of leaky sites, when compared to controls (C—filtered water without vitamin E in the chow) (*p<0.01- F vs. C). Supplementation with vitamin E restored microcirculatory function, as improvements may be seen on both fructose (#p<0.01 –F vs. F/5XVE) and control responses (+p<0.01 –C vs. C/5XVE).

Mentions: Microvascular permeability induced by I/R and histamine also presented significant differences between the 2 groups investigated. Improvements on both ischemia/reperfusion and histamine-induced macromolecular permeability are illustrated on Fig 4A and 4B. The number of leaky sites after I/R procedure in fructose-drinking/without vitamin E animals showed an increase of only 11%, compared to its paired controls (*p<0.05 –C vs. F), while more substantial improvement occurred when supplementation with vitamin E was added. Controls and 10% fructose-drinking animals with normal and supplemented chows had significant decrease in the number leaky sites, in the order of 20% in control (*p<0.01 –C vs. C/VE and C/5XVE), and (F/VE) and (F/5XVE) in fructose-drinking groups (#p<0.01 –F vs. F/VE and F/5XVE).


α-Tocopherol Improves Microcirculatory Dysfunction on Fructose Fed Hamsters.

Boa BC, Barros CM, Souza Md, Castiglione RC, Cyrino FZ, Bouskela E - PLoS ONE (2015)

Microvascular permeability measurements after I/R procedure and topical application of histamine.Data are shown as number of leaks, expressed as mean ± SD and plotted in column bars. (A) Number of extravasations after 30 min ischemia followed by reperfusion, 10 minutes after the onset of reperfusion. Significant differences were found between *C vs. F (p<0.01), + C vs. C/VE and C/5XVE (p<0.001), and F vs. F/VE and F/5XVE (p<0.01). (B) Histamine-mediated microvascular permeability. Animals treated with 10% fructose solution without vitamin E in the chow presented higher number of leaky sites, when compared to controls (C—filtered water without vitamin E in the chow) (*p<0.01- F vs. C). Supplementation with vitamin E restored microcirculatory function, as improvements may be seen on both fructose (#p<0.01 –F vs. F/5XVE) and control responses (+p<0.01 –C vs. C/5XVE).
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4526657&req=5

pone.0134740.g004: Microvascular permeability measurements after I/R procedure and topical application of histamine.Data are shown as number of leaks, expressed as mean ± SD and plotted in column bars. (A) Number of extravasations after 30 min ischemia followed by reperfusion, 10 minutes after the onset of reperfusion. Significant differences were found between *C vs. F (p<0.01), + C vs. C/VE and C/5XVE (p<0.001), and F vs. F/VE and F/5XVE (p<0.01). (B) Histamine-mediated microvascular permeability. Animals treated with 10% fructose solution without vitamin E in the chow presented higher number of leaky sites, when compared to controls (C—filtered water without vitamin E in the chow) (*p<0.01- F vs. C). Supplementation with vitamin E restored microcirculatory function, as improvements may be seen on both fructose (#p<0.01 –F vs. F/5XVE) and control responses (+p<0.01 –C vs. C/5XVE).
Mentions: Microvascular permeability induced by I/R and histamine also presented significant differences between the 2 groups investigated. Improvements on both ischemia/reperfusion and histamine-induced macromolecular permeability are illustrated on Fig 4A and 4B. The number of leaky sites after I/R procedure in fructose-drinking/without vitamin E animals showed an increase of only 11%, compared to its paired controls (*p<0.05 –C vs. F), while more substantial improvement occurred when supplementation with vitamin E was added. Controls and 10% fructose-drinking animals with normal and supplemented chows had significant decrease in the number leaky sites, in the order of 20% in control (*p<0.01 –C vs. C/VE and C/5XVE), and (F/VE) and (F/5XVE) in fructose-drinking groups (#p<0.01 –F vs. F/VE and F/5XVE).

Bottom Line: On the other hand, vitamin E supplementation resulted in increased responses for both water and fructose drinking groups (177.4% for F vs.The number of leaky sites after I/R and histamine stimuli in vitamin E supplemented animals decreased (-25.1% and -15.3% for F vs.Our results strongly suggest that vitamin E could improve the endothelial function and permeability barrier and also reverse impairments elicited by sugar overload.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Clinical and Experimental Research on Vascular Biology (BioVasc), Biomedical Center, State University of Rio de Janeiro, Rio de Janeiro, Brazil.

ABSTRACT
Fructose, an everyday component of western diet associated to chronic hyperglycemia and enhanced free radical production, impairs endothelial function and supplementation with antioxidants might improve it. In this study we investigated if vitamin E could reverse the microvascular damage elicited by fructose. Male Syrian golden hamsters drank either 10% fructose solution (F) or filtered water (C), combined with three concentrations of vitamin E in their chows [zero, normal (VE) or 5X (5XVE)] during 60 days. Microvascular reactivity in response to topical application of acetylcholine (Ach; endothelium-dependent vasodilator) or sodium nitroprusside (SNP; endothelium-independent vasodilator) and macromolecular permeability increase induced by either 30 min ischemia followed by reperfusion (I/R) or topical application of histamine (5 μM) were assessed using the cheek pouch preparation. Compared to controls (drinking filtered water), fructose-drinking animals showed decreased vasodilatation to acetylcholine in all concentrations tested (-56.2% for 10-9M, -53.9% for 10-7M and -43.7% for 10-5M). On the other hand, vitamin E supplementation resulted in increased responses for both water and fructose drinking groups (177.4% for F vs. F/5XVE and 241.6% for C vs. C/5XVE for 10-5M Ach). Endothelial-independent vasodilatation explored by topical application of SNP was restored and even enhanced with the supplementation of 5X vitamin E in both groups (80.1% for F vs. F/5XVE; 144.2% for C vs. C/5XVE; 3.4% of difference for C/5XVE vs. F/5XVE on 10-5M SNP). The number of leaky sites after I/R and histamine stimuli in vitamin E supplemented animals decreased (-25.1% and -15.3% for F vs. F/5XVE; and -21.7% and -16% of leaky sites comparing C vs. C/5XVE, respectively for I/R and histamine stimuli) pointing to tightening of the endothelial barrier for macromolecular permeability. Our results strongly suggest that vitamin E could improve the endothelial function and permeability barrier and also reverse impairments elicited by sugar overload.

No MeSH data available.


Related in: MedlinePlus