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Long-Term Extensive Ectopic Hair Growth on the Spinal Cord of Mice from Transplanted Whisker Follicles.

Cao W, Li L, Mii S, Amoh Y, Liu F, Hoffman RM - PLoS ONE (2015)

Bottom Line: ND-GFP expression was intense at the healed area of the spinal cord, as observed by fluorescence microscopy, demonstrating that the hair follicle stem cells were involved in healing the spinal cord.The pigmented hair fibers, grown from the transplanted whisker histocultures, curved and enclosed the spinal cord.The unanticipated results demonstrate the great potential of hair growth after transplantation of Gelfoam hair follicle histocultures, even at an ectopic site.

View Article: PubMed Central - PubMed

Affiliation: AntiCancer Inc., San Diego, CA, United States of America; Department of Surgery, University of California San Diego, San Diego, CA, United States of America; Department of Anatomy, Second Military Medical University, Shanghai, China.

ABSTRACT
We have previously demonstrated that hair follicles contain nestin-expressing pluripotent stem cells that can effect nerve and spinal cord repair upon transplantation. In the present study, isolated whisker follicles from nestin-driven green fluorescent protein (ND-GFP) mice were histocultured on Gelfoam for 3 weeks for the purpose of transplantation to the spinal cord to heal an induced injury. The hair shaft was cut off from Gelfoam-histocultured whisker follicles, and the remaining part of the whisker follicles containing GFP-nestin expressing pluripotent stem cells were transplanted into the injured spinal cord of nude mice, along with the Gelfoam. After 90 days, the mice were sacrificed and the spinal cord lesion was observed to have healed. ND-GFP expression was intense at the healed area of the spinal cord, as observed by fluorescence microscopy, demonstrating that the hair follicle stem cells were involved in healing the spinal cord. Unexpectedly, the transplanted whisker follicles sprouted out remarkably long hair shafts in the spinal cord during the 90 days after transplantation of Gelfoam whisker histocultures to the injured spine. The pigmented hair fibers, grown from the transplanted whisker histocultures, curved and enclosed the spinal cord. The unanticipated results demonstrate the great potential of hair growth after transplantation of Gelfoam hair follicle histocultures, even at an ectopic site.

No MeSH data available.


Related in: MedlinePlus

Transplantation of nestin-driven green fluorescent protein (ND-GFP)-expressing hair follicle Gelfoam histocultures to the injured spinal cord of nude mice.After Gelfoam histoculture of isolated whisker hair follicles from nestin-driven GFP (ND-GFP) mice for 3 weeks, the long hair shafts of the whisker follicle were cut off, and the follicle, along with the Gelfoam, was transplanted into the injured nude-mouse spinal cord. The transplanted mouse was sacrificed after 90 days. ND-GFP expression intensified by 90 days and expanded in the injured area of the spinal cord, which was apparently healed by the ND-GFP expressing stem cells. A total of 7 mice were studied. The figure shows typical data.
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pone.0133475.g001: Transplantation of nestin-driven green fluorescent protein (ND-GFP)-expressing hair follicle Gelfoam histocultures to the injured spinal cord of nude mice.After Gelfoam histoculture of isolated whisker hair follicles from nestin-driven GFP (ND-GFP) mice for 3 weeks, the long hair shafts of the whisker follicle were cut off, and the follicle, along with the Gelfoam, was transplanted into the injured nude-mouse spinal cord. The transplanted mouse was sacrificed after 90 days. ND-GFP expression intensified by 90 days and expanded in the injured area of the spinal cord, which was apparently healed by the ND-GFP expressing stem cells. A total of 7 mice were studied. The figure shows typical data.

Mentions: After Gelfoam histoculture of whiskers for 3 weeks, elongated hair shafts were cut off and the whisker follicles, containing ND-GFP stem cells, which had increased during histoculture, were transplanted along with the Gelfoam into the injured spinal cord of nude mice (Fig 1).


Long-Term Extensive Ectopic Hair Growth on the Spinal Cord of Mice from Transplanted Whisker Follicles.

Cao W, Li L, Mii S, Amoh Y, Liu F, Hoffman RM - PLoS ONE (2015)

Transplantation of nestin-driven green fluorescent protein (ND-GFP)-expressing hair follicle Gelfoam histocultures to the injured spinal cord of nude mice.After Gelfoam histoculture of isolated whisker hair follicles from nestin-driven GFP (ND-GFP) mice for 3 weeks, the long hair shafts of the whisker follicle were cut off, and the follicle, along with the Gelfoam, was transplanted into the injured nude-mouse spinal cord. The transplanted mouse was sacrificed after 90 days. ND-GFP expression intensified by 90 days and expanded in the injured area of the spinal cord, which was apparently healed by the ND-GFP expressing stem cells. A total of 7 mice were studied. The figure shows typical data.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526646&req=5

pone.0133475.g001: Transplantation of nestin-driven green fluorescent protein (ND-GFP)-expressing hair follicle Gelfoam histocultures to the injured spinal cord of nude mice.After Gelfoam histoculture of isolated whisker hair follicles from nestin-driven GFP (ND-GFP) mice for 3 weeks, the long hair shafts of the whisker follicle were cut off, and the follicle, along with the Gelfoam, was transplanted into the injured nude-mouse spinal cord. The transplanted mouse was sacrificed after 90 days. ND-GFP expression intensified by 90 days and expanded in the injured area of the spinal cord, which was apparently healed by the ND-GFP expressing stem cells. A total of 7 mice were studied. The figure shows typical data.
Mentions: After Gelfoam histoculture of whiskers for 3 weeks, elongated hair shafts were cut off and the whisker follicles, containing ND-GFP stem cells, which had increased during histoculture, were transplanted along with the Gelfoam into the injured spinal cord of nude mice (Fig 1).

Bottom Line: ND-GFP expression was intense at the healed area of the spinal cord, as observed by fluorescence microscopy, demonstrating that the hair follicle stem cells were involved in healing the spinal cord.The pigmented hair fibers, grown from the transplanted whisker histocultures, curved and enclosed the spinal cord.The unanticipated results demonstrate the great potential of hair growth after transplantation of Gelfoam hair follicle histocultures, even at an ectopic site.

View Article: PubMed Central - PubMed

Affiliation: AntiCancer Inc., San Diego, CA, United States of America; Department of Surgery, University of California San Diego, San Diego, CA, United States of America; Department of Anatomy, Second Military Medical University, Shanghai, China.

ABSTRACT
We have previously demonstrated that hair follicles contain nestin-expressing pluripotent stem cells that can effect nerve and spinal cord repair upon transplantation. In the present study, isolated whisker follicles from nestin-driven green fluorescent protein (ND-GFP) mice were histocultured on Gelfoam for 3 weeks for the purpose of transplantation to the spinal cord to heal an induced injury. The hair shaft was cut off from Gelfoam-histocultured whisker follicles, and the remaining part of the whisker follicles containing GFP-nestin expressing pluripotent stem cells were transplanted into the injured spinal cord of nude mice, along with the Gelfoam. After 90 days, the mice were sacrificed and the spinal cord lesion was observed to have healed. ND-GFP expression was intense at the healed area of the spinal cord, as observed by fluorescence microscopy, demonstrating that the hair follicle stem cells were involved in healing the spinal cord. Unexpectedly, the transplanted whisker follicles sprouted out remarkably long hair shafts in the spinal cord during the 90 days after transplantation of Gelfoam whisker histocultures to the injured spine. The pigmented hair fibers, grown from the transplanted whisker histocultures, curved and enclosed the spinal cord. The unanticipated results demonstrate the great potential of hair growth after transplantation of Gelfoam hair follicle histocultures, even at an ectopic site.

No MeSH data available.


Related in: MedlinePlus