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Stress Tolerance Variations in Saccharomyces cerevisiae Strains from Diverse Ecological Sources and Geographical Locations.

Zheng YL, Wang SA - PLoS ONE (2015)

Bottom Line: The results showed that the isolates from human-associated environments overall presented a higher level of stress tolerance compared with those from forests spared anthropogenic influences.Statistical analyses indicated that the variations of stress tolerance were significantly correlated with both ecological sources and geographical locations of the strains.This study provides guidelines for selection of robust S. cerevisiae strains for bioethanol production from nature.

View Article: PubMed Central - PubMed

Affiliation: College of Mathematics and Systems Science, Shandong University of Science and Technology, Qingdao, 266590, China.

ABSTRACT
The budding yeast Saccharomyces cerevisiae is a platform organism for bioethanol production from various feedstocks and robust strains are desirable for efficient fermentation because yeast cells inevitably encounter stressors during the process. Recently, diverse S. cerevisiae lineages were identified, which provided novel resources for understanding stress tolerance variations and related shaping factors in the yeast. This study characterized the tolerance of diverse S. cerevisiae strains to the stressors of high ethanol concentrations, temperature shocks, and osmotic stress. The results showed that the isolates from human-associated environments overall presented a higher level of stress tolerance compared with those from forests spared anthropogenic influences. Statistical analyses indicated that the variations of stress tolerance were significantly correlated with both ecological sources and geographical locations of the strains. This study provides guidelines for selection of robust S. cerevisiae strains for bioethanol production from nature.

No MeSH data available.


Related in: MedlinePlus

Boxplots depicting stress tolerance variations in the Wild group.(a) ethanol stress; (b) heat stress; (c) osmotic stress. ○ = values >1.5 box-lengths from the box but not extremes (outliers). * Values >3 box-lengths from the box (extremes).
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pone.0133889.g005: Boxplots depicting stress tolerance variations in the Wild group.(a) ethanol stress; (b) heat stress; (c) osmotic stress. ○ = values >1.5 box-lengths from the box but not extremes (outliers). * Values >3 box-lengths from the box (extremes).

Mentions: To investigate influences of geographic location on stress tolerance variations, strains in an identical ecological group were divided into sub-groups based on geographic locations (provinces) (Table 1 and S3 Fig). Significant differences (ANOVA, at the 0.05 level) presented among the sub-groups in both the Wild group and the Orchard group (Fig 5 and Fig 6). For example, the Wild strains isolated from Beijing and Shandong province displayed a significantly (ANOVA, at the 0.01 level) higher level of tolerance to ethanol and heat stress compared with those Wild strains (lineage CHN II) from Shaanxi province (Fig 5). The Pearson correlation coefficient (r) to the variability of ethanol and heat stress was 0.51 and 0.77, from which 25.67% and 59.98% of the variability could be explained by geographic locations, respectively. The Orchard isolates from Xinjiang province presented significantly (ANOVA, at the 0.05 level) higher sensitivity to osmotic stress compared with those Orchard isolates in other sub-groups (Fig 6). The Pearson correlation coefficient to the variability of osmotic stress was 0.78, from which 60.73% of the variability could be explained by geographic locations. These results indicated the correlation between phenotypic variations of S. cerevisiae strains and geographic location.


Stress Tolerance Variations in Saccharomyces cerevisiae Strains from Diverse Ecological Sources and Geographical Locations.

Zheng YL, Wang SA - PLoS ONE (2015)

Boxplots depicting stress tolerance variations in the Wild group.(a) ethanol stress; (b) heat stress; (c) osmotic stress. ○ = values >1.5 box-lengths from the box but not extremes (outliers). * Values >3 box-lengths from the box (extremes).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526645&req=5

pone.0133889.g005: Boxplots depicting stress tolerance variations in the Wild group.(a) ethanol stress; (b) heat stress; (c) osmotic stress. ○ = values >1.5 box-lengths from the box but not extremes (outliers). * Values >3 box-lengths from the box (extremes).
Mentions: To investigate influences of geographic location on stress tolerance variations, strains in an identical ecological group were divided into sub-groups based on geographic locations (provinces) (Table 1 and S3 Fig). Significant differences (ANOVA, at the 0.05 level) presented among the sub-groups in both the Wild group and the Orchard group (Fig 5 and Fig 6). For example, the Wild strains isolated from Beijing and Shandong province displayed a significantly (ANOVA, at the 0.01 level) higher level of tolerance to ethanol and heat stress compared with those Wild strains (lineage CHN II) from Shaanxi province (Fig 5). The Pearson correlation coefficient (r) to the variability of ethanol and heat stress was 0.51 and 0.77, from which 25.67% and 59.98% of the variability could be explained by geographic locations, respectively. The Orchard isolates from Xinjiang province presented significantly (ANOVA, at the 0.05 level) higher sensitivity to osmotic stress compared with those Orchard isolates in other sub-groups (Fig 6). The Pearson correlation coefficient to the variability of osmotic stress was 0.78, from which 60.73% of the variability could be explained by geographic locations. These results indicated the correlation between phenotypic variations of S. cerevisiae strains and geographic location.

Bottom Line: The results showed that the isolates from human-associated environments overall presented a higher level of stress tolerance compared with those from forests spared anthropogenic influences.Statistical analyses indicated that the variations of stress tolerance were significantly correlated with both ecological sources and geographical locations of the strains.This study provides guidelines for selection of robust S. cerevisiae strains for bioethanol production from nature.

View Article: PubMed Central - PubMed

Affiliation: College of Mathematics and Systems Science, Shandong University of Science and Technology, Qingdao, 266590, China.

ABSTRACT
The budding yeast Saccharomyces cerevisiae is a platform organism for bioethanol production from various feedstocks and robust strains are desirable for efficient fermentation because yeast cells inevitably encounter stressors during the process. Recently, diverse S. cerevisiae lineages were identified, which provided novel resources for understanding stress tolerance variations and related shaping factors in the yeast. This study characterized the tolerance of diverse S. cerevisiae strains to the stressors of high ethanol concentrations, temperature shocks, and osmotic stress. The results showed that the isolates from human-associated environments overall presented a higher level of stress tolerance compared with those from forests spared anthropogenic influences. Statistical analyses indicated that the variations of stress tolerance were significantly correlated with both ecological sources and geographical locations of the strains. This study provides guidelines for selection of robust S. cerevisiae strains for bioethanol production from nature.

No MeSH data available.


Related in: MedlinePlus