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Stress Tolerance Variations in Saccharomyces cerevisiae Strains from Diverse Ecological Sources and Geographical Locations.

Zheng YL, Wang SA - PLoS ONE (2015)

Bottom Line: The results showed that the isolates from human-associated environments overall presented a higher level of stress tolerance compared with those from forests spared anthropogenic influences.Statistical analyses indicated that the variations of stress tolerance were significantly correlated with both ecological sources and geographical locations of the strains.This study provides guidelines for selection of robust S. cerevisiae strains for bioethanol production from nature.

View Article: PubMed Central - PubMed

Affiliation: College of Mathematics and Systems Science, Shandong University of Science and Technology, Qingdao, 266590, China.

ABSTRACT
The budding yeast Saccharomyces cerevisiae is a platform organism for bioethanol production from various feedstocks and robust strains are desirable for efficient fermentation because yeast cells inevitably encounter stressors during the process. Recently, diverse S. cerevisiae lineages were identified, which provided novel resources for understanding stress tolerance variations and related shaping factors in the yeast. This study characterized the tolerance of diverse S. cerevisiae strains to the stressors of high ethanol concentrations, temperature shocks, and osmotic stress. The results showed that the isolates from human-associated environments overall presented a higher level of stress tolerance compared with those from forests spared anthropogenic influences. Statistical analyses indicated that the variations of stress tolerance were significantly correlated with both ecological sources and geographical locations of the strains. This study provides guidelines for selection of robust S. cerevisiae strains for bioethanol production from nature.

No MeSH data available.


Related in: MedlinePlus

Pearson correlation between ethanol and heat stress tolerance determined by bivariate correlation analysis.Ethanol and heat stress tolerance was inferred from the biomass (gram) of 3 ml cultures grown for 72h under ethanol or heat stress.
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pone.0133889.g003: Pearson correlation between ethanol and heat stress tolerance determined by bivariate correlation analysis.Ethanol and heat stress tolerance was inferred from the biomass (gram) of 3 ml cultures grown for 72h under ethanol or heat stress.

Mentions: The range of biomass variations under stresses of ethanol, heat, and osmotic stress were 0.3 to 3.7 mg, 0.17 to 3.1 mg, and 0.54 to 1.9 mg, respectively. These data displayed a larger variation on tolerance to ethanol or heat compared with osmotic stress in S. cerevisiae populations (Fig 2). Bivariate correlation analysis represented a high correlation (Pearson r = 0.71 at p<0.0001) between ethanol and heat stress tolerance (Fig 3). This finding was similar (Pearson r = 0.51 at p<0.0006) to that deduced from testing a panel of strains from vineyard, sake and clinical environments [15]. Overlapping stress responses could be induced by heat and ethanol shock, such as causing similar changes to plasma membrane protein composition and inducing heat shock proteins that contribute to both thermal and ethanol tolerance [32]. These phenomena can be explained by environmental modification of S. cerevisiae via fermentation and the subsequent selection pressures imposed on them by the production of both ethanol and heat together [33,34].


Stress Tolerance Variations in Saccharomyces cerevisiae Strains from Diverse Ecological Sources and Geographical Locations.

Zheng YL, Wang SA - PLoS ONE (2015)

Pearson correlation between ethanol and heat stress tolerance determined by bivariate correlation analysis.Ethanol and heat stress tolerance was inferred from the biomass (gram) of 3 ml cultures grown for 72h under ethanol or heat stress.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526645&req=5

pone.0133889.g003: Pearson correlation between ethanol and heat stress tolerance determined by bivariate correlation analysis.Ethanol and heat stress tolerance was inferred from the biomass (gram) of 3 ml cultures grown for 72h under ethanol or heat stress.
Mentions: The range of biomass variations under stresses of ethanol, heat, and osmotic stress were 0.3 to 3.7 mg, 0.17 to 3.1 mg, and 0.54 to 1.9 mg, respectively. These data displayed a larger variation on tolerance to ethanol or heat compared with osmotic stress in S. cerevisiae populations (Fig 2). Bivariate correlation analysis represented a high correlation (Pearson r = 0.71 at p<0.0001) between ethanol and heat stress tolerance (Fig 3). This finding was similar (Pearson r = 0.51 at p<0.0006) to that deduced from testing a panel of strains from vineyard, sake and clinical environments [15]. Overlapping stress responses could be induced by heat and ethanol shock, such as causing similar changes to plasma membrane protein composition and inducing heat shock proteins that contribute to both thermal and ethanol tolerance [32]. These phenomena can be explained by environmental modification of S. cerevisiae via fermentation and the subsequent selection pressures imposed on them by the production of both ethanol and heat together [33,34].

Bottom Line: The results showed that the isolates from human-associated environments overall presented a higher level of stress tolerance compared with those from forests spared anthropogenic influences.Statistical analyses indicated that the variations of stress tolerance were significantly correlated with both ecological sources and geographical locations of the strains.This study provides guidelines for selection of robust S. cerevisiae strains for bioethanol production from nature.

View Article: PubMed Central - PubMed

Affiliation: College of Mathematics and Systems Science, Shandong University of Science and Technology, Qingdao, 266590, China.

ABSTRACT
The budding yeast Saccharomyces cerevisiae is a platform organism for bioethanol production from various feedstocks and robust strains are desirable for efficient fermentation because yeast cells inevitably encounter stressors during the process. Recently, diverse S. cerevisiae lineages were identified, which provided novel resources for understanding stress tolerance variations and related shaping factors in the yeast. This study characterized the tolerance of diverse S. cerevisiae strains to the stressors of high ethanol concentrations, temperature shocks, and osmotic stress. The results showed that the isolates from human-associated environments overall presented a higher level of stress tolerance compared with those from forests spared anthropogenic influences. Statistical analyses indicated that the variations of stress tolerance were significantly correlated with both ecological sources and geographical locations of the strains. This study provides guidelines for selection of robust S. cerevisiae strains for bioethanol production from nature.

No MeSH data available.


Related in: MedlinePlus