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Stress Tolerance Variations in Saccharomyces cerevisiae Strains from Diverse Ecological Sources and Geographical Locations.

Zheng YL, Wang SA - PLoS ONE (2015)

Bottom Line: The results showed that the isolates from human-associated environments overall presented a higher level of stress tolerance compared with those from forests spared anthropogenic influences.Statistical analyses indicated that the variations of stress tolerance were significantly correlated with both ecological sources and geographical locations of the strains.This study provides guidelines for selection of robust S. cerevisiae strains for bioethanol production from nature.

View Article: PubMed Central - PubMed

Affiliation: College of Mathematics and Systems Science, Shandong University of Science and Technology, Qingdao, 266590, China.

ABSTRACT
The budding yeast Saccharomyces cerevisiae is a platform organism for bioethanol production from various feedstocks and robust strains are desirable for efficient fermentation because yeast cells inevitably encounter stressors during the process. Recently, diverse S. cerevisiae lineages were identified, which provided novel resources for understanding stress tolerance variations and related shaping factors in the yeast. This study characterized the tolerance of diverse S. cerevisiae strains to the stressors of high ethanol concentrations, temperature shocks, and osmotic stress. The results showed that the isolates from human-associated environments overall presented a higher level of stress tolerance compared with those from forests spared anthropogenic influences. Statistical analyses indicated that the variations of stress tolerance were significantly correlated with both ecological sources and geographical locations of the strains. This study provides guidelines for selection of robust S. cerevisiae strains for bioethanol production from nature.

No MeSH data available.


Related in: MedlinePlus

Stress tolerance variations in the S. cerevisiae isolates.Hierarchical clustering of phenotypes was performed using a centered Pearson correlation metric and average linkage mapping. Biomass of strains cultured for 72h in stressful conditions was used to infer stress tolerance. Locations of the isolates were also depicted. The industrial strains from multiple locations were not subdivided.
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pone.0133889.g002: Stress tolerance variations in the S. cerevisiae isolates.Hierarchical clustering of phenotypes was performed using a centered Pearson correlation metric and average linkage mapping. Biomass of strains cultured for 72h in stressful conditions was used to infer stress tolerance. Locations of the isolates were also depicted. The industrial strains from multiple locations were not subdivided.

Mentions: Hierarchical clustering of the 83 strains of S. cerevisiae was performed according to biomass dry weight data (Fig 2). Five clusters were recognized and designated as A to E. Most strains (40/60) in an identical genetic lineage were grouped in an identical phenotypic cluster. All isolates in lineages CHN I, CHN II, CHN III, and Clade 3 gathered in cluster B; all isolates in lineage CHN VIII and Clade 1 gathered in cluster D and E, respectively; The Clade 2 isolates excluding XL4 were placed in cluster D. Varied stress tolerance was shown in lineages CHN IV, CHN V and CHN VI, in which the isolates were placed in clusters B to E (Fig 2). These findings indicated correspondence between genotype and phenotype in lineages of S. cerevisiae.


Stress Tolerance Variations in Saccharomyces cerevisiae Strains from Diverse Ecological Sources and Geographical Locations.

Zheng YL, Wang SA - PLoS ONE (2015)

Stress tolerance variations in the S. cerevisiae isolates.Hierarchical clustering of phenotypes was performed using a centered Pearson correlation metric and average linkage mapping. Biomass of strains cultured for 72h in stressful conditions was used to infer stress tolerance. Locations of the isolates were also depicted. The industrial strains from multiple locations were not subdivided.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526645&req=5

pone.0133889.g002: Stress tolerance variations in the S. cerevisiae isolates.Hierarchical clustering of phenotypes was performed using a centered Pearson correlation metric and average linkage mapping. Biomass of strains cultured for 72h in stressful conditions was used to infer stress tolerance. Locations of the isolates were also depicted. The industrial strains from multiple locations were not subdivided.
Mentions: Hierarchical clustering of the 83 strains of S. cerevisiae was performed according to biomass dry weight data (Fig 2). Five clusters were recognized and designated as A to E. Most strains (40/60) in an identical genetic lineage were grouped in an identical phenotypic cluster. All isolates in lineages CHN I, CHN II, CHN III, and Clade 3 gathered in cluster B; all isolates in lineage CHN VIII and Clade 1 gathered in cluster D and E, respectively; The Clade 2 isolates excluding XL4 were placed in cluster D. Varied stress tolerance was shown in lineages CHN IV, CHN V and CHN VI, in which the isolates were placed in clusters B to E (Fig 2). These findings indicated correspondence between genotype and phenotype in lineages of S. cerevisiae.

Bottom Line: The results showed that the isolates from human-associated environments overall presented a higher level of stress tolerance compared with those from forests spared anthropogenic influences.Statistical analyses indicated that the variations of stress tolerance were significantly correlated with both ecological sources and geographical locations of the strains.This study provides guidelines for selection of robust S. cerevisiae strains for bioethanol production from nature.

View Article: PubMed Central - PubMed

Affiliation: College of Mathematics and Systems Science, Shandong University of Science and Technology, Qingdao, 266590, China.

ABSTRACT
The budding yeast Saccharomyces cerevisiae is a platform organism for bioethanol production from various feedstocks and robust strains are desirable for efficient fermentation because yeast cells inevitably encounter stressors during the process. Recently, diverse S. cerevisiae lineages were identified, which provided novel resources for understanding stress tolerance variations and related shaping factors in the yeast. This study characterized the tolerance of diverse S. cerevisiae strains to the stressors of high ethanol concentrations, temperature shocks, and osmotic stress. The results showed that the isolates from human-associated environments overall presented a higher level of stress tolerance compared with those from forests spared anthropogenic influences. Statistical analyses indicated that the variations of stress tolerance were significantly correlated with both ecological sources and geographical locations of the strains. This study provides guidelines for selection of robust S. cerevisiae strains for bioethanol production from nature.

No MeSH data available.


Related in: MedlinePlus