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Stress Tolerance Variations in Saccharomyces cerevisiae Strains from Diverse Ecological Sources and Geographical Locations.

Zheng YL, Wang SA - PLoS ONE (2015)

Bottom Line: The results showed that the isolates from human-associated environments overall presented a higher level of stress tolerance compared with those from forests spared anthropogenic influences.Statistical analyses indicated that the variations of stress tolerance were significantly correlated with both ecological sources and geographical locations of the strains.This study provides guidelines for selection of robust S. cerevisiae strains for bioethanol production from nature.

View Article: PubMed Central - PubMed

Affiliation: College of Mathematics and Systems Science, Shandong University of Science and Technology, Qingdao, 266590, China.

ABSTRACT
The budding yeast Saccharomyces cerevisiae is a platform organism for bioethanol production from various feedstocks and robust strains are desirable for efficient fermentation because yeast cells inevitably encounter stressors during the process. Recently, diverse S. cerevisiae lineages were identified, which provided novel resources for understanding stress tolerance variations and related shaping factors in the yeast. This study characterized the tolerance of diverse S. cerevisiae strains to the stressors of high ethanol concentrations, temperature shocks, and osmotic stress. The results showed that the isolates from human-associated environments overall presented a higher level of stress tolerance compared with those from forests spared anthropogenic influences. Statistical analyses indicated that the variations of stress tolerance were significantly correlated with both ecological sources and geographical locations of the strains. This study provides guidelines for selection of robust S. cerevisiae strains for bioethanol production from nature.

No MeSH data available.


Related in: MedlinePlus

Neighbor-joining tree constructed from concatenated sequences of gene loci, for which neutral evolution was not rejected.Bootstrap percentages over 50% from 1000 bootstrap replicates were shown. Saccharomyces paradoxus was used as the out-group. Light grey shadow denotes the world lineages, tawny shadow denotes the Chinese lineages, and purple shadow denotes the Clades proposed in this study.
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pone.0133889.g001: Neighbor-joining tree constructed from concatenated sequences of gene loci, for which neutral evolution was not rejected.Bootstrap percentages over 50% from 1000 bootstrap replicates were shown. Saccharomyces paradoxus was used as the out-group. Light grey shadow denotes the world lineages, tawny shadow denotes the Chinese lineages, and purple shadow denotes the Clades proposed in this study.

Mentions: Phylogenetic relationships among strains of S. cerevisiae were inferred from a neighbor-joining (NJ) tree constructed from concatenated sequences of gene loci, for which neutral evolution was not rejected, namely ACT1, HSP104, RPB1, RPN2, TBP, and the synonymous sites in TPS1. For the strains containing heterozygous sites, one randomly selected haplotype was used in the analysis. Seven of the eight Chinese lineages and the five world lineages previously proposed were depicted in the NJ tree (Fig 1). The strains in Chinese lineage CHN IV were separated into two clades in this study, which should be due to the used gene loci different from previous study for phylogenetic analysis [11]. In addition to the previous reported lineages, three other clades with strong bootstrap values (>92%) were identified and designated as Clade 1, Clade2, and Clade 3 (Fig 1). The Clade 1 and Clade2 strains were isolated from fruits in northern China and western China, respectively, while the Clade3 strains constituted beer yeasts from England (Table 1). Structure analysis supported the general groupings of the strains (S1 Fig). Additional gene loci or genome sequences are needed to estimate if the three Clades are novel S. cerevisiae lineages.


Stress Tolerance Variations in Saccharomyces cerevisiae Strains from Diverse Ecological Sources and Geographical Locations.

Zheng YL, Wang SA - PLoS ONE (2015)

Neighbor-joining tree constructed from concatenated sequences of gene loci, for which neutral evolution was not rejected.Bootstrap percentages over 50% from 1000 bootstrap replicates were shown. Saccharomyces paradoxus was used as the out-group. Light grey shadow denotes the world lineages, tawny shadow denotes the Chinese lineages, and purple shadow denotes the Clades proposed in this study.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526645&req=5

pone.0133889.g001: Neighbor-joining tree constructed from concatenated sequences of gene loci, for which neutral evolution was not rejected.Bootstrap percentages over 50% from 1000 bootstrap replicates were shown. Saccharomyces paradoxus was used as the out-group. Light grey shadow denotes the world lineages, tawny shadow denotes the Chinese lineages, and purple shadow denotes the Clades proposed in this study.
Mentions: Phylogenetic relationships among strains of S. cerevisiae were inferred from a neighbor-joining (NJ) tree constructed from concatenated sequences of gene loci, for which neutral evolution was not rejected, namely ACT1, HSP104, RPB1, RPN2, TBP, and the synonymous sites in TPS1. For the strains containing heterozygous sites, one randomly selected haplotype was used in the analysis. Seven of the eight Chinese lineages and the five world lineages previously proposed were depicted in the NJ tree (Fig 1). The strains in Chinese lineage CHN IV were separated into two clades in this study, which should be due to the used gene loci different from previous study for phylogenetic analysis [11]. In addition to the previous reported lineages, three other clades with strong bootstrap values (>92%) were identified and designated as Clade 1, Clade2, and Clade 3 (Fig 1). The Clade 1 and Clade2 strains were isolated from fruits in northern China and western China, respectively, while the Clade3 strains constituted beer yeasts from England (Table 1). Structure analysis supported the general groupings of the strains (S1 Fig). Additional gene loci or genome sequences are needed to estimate if the three Clades are novel S. cerevisiae lineages.

Bottom Line: The results showed that the isolates from human-associated environments overall presented a higher level of stress tolerance compared with those from forests spared anthropogenic influences.Statistical analyses indicated that the variations of stress tolerance were significantly correlated with both ecological sources and geographical locations of the strains.This study provides guidelines for selection of robust S. cerevisiae strains for bioethanol production from nature.

View Article: PubMed Central - PubMed

Affiliation: College of Mathematics and Systems Science, Shandong University of Science and Technology, Qingdao, 266590, China.

ABSTRACT
The budding yeast Saccharomyces cerevisiae is a platform organism for bioethanol production from various feedstocks and robust strains are desirable for efficient fermentation because yeast cells inevitably encounter stressors during the process. Recently, diverse S. cerevisiae lineages were identified, which provided novel resources for understanding stress tolerance variations and related shaping factors in the yeast. This study characterized the tolerance of diverse S. cerevisiae strains to the stressors of high ethanol concentrations, temperature shocks, and osmotic stress. The results showed that the isolates from human-associated environments overall presented a higher level of stress tolerance compared with those from forests spared anthropogenic influences. Statistical analyses indicated that the variations of stress tolerance were significantly correlated with both ecological sources and geographical locations of the strains. This study provides guidelines for selection of robust S. cerevisiae strains for bioethanol production from nature.

No MeSH data available.


Related in: MedlinePlus