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Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s.

Hujaya SD, Engbersen JF, Paulusse JM - Pharm. Res. (2015)

Bottom Line: Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers.Graphical Abstract Layer-by-Layer Assembly.

View Article: PubMed Central - PubMed

Affiliation: Department of Controlled Drug Delivery, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands.

ABSTRACT

Purpose: To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells.

Methods: Copolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.

Results: PVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10-100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells.

Conclusions: Responsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants. Graphical Abstract Layer-by-Layer Assembly.

No MeSH data available.


Related in: MedlinePlus

Stability profiles of: (a) PVA- and (b) ChS-based multilayers under physiological conditions (PBS pH 7.4 at 37°C) and in the presence of glutathione (0.4 and 10 mM) or DTT (2.5 mM). The left part of the abscissa shows the trend of fast decrease (burst release) within 0.5 h (30 min) in the initial phase of the incubation while the right part shows the much slower decrease throughout the rest of the long incubation duration.
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Fig9: Stability profiles of: (a) PVA- and (b) ChS-based multilayers under physiological conditions (PBS pH 7.4 at 37°C) and in the presence of glutathione (0.4 and 10 mM) or DTT (2.5 mM). The left part of the abscissa shows the trend of fast decrease (burst release) within 0.5 h (30 min) in the initial phase of the incubation while the right part shows the much slower decrease throughout the rest of the long incubation duration.

Mentions: Figure 9 shows the decrease in relative absorbance values in time, i.e., the ratio of absorbance value against the respective value at time zero. Figure 9a and b show the results for PVA- and ChS-based systems, respectively. Throughout the incubation time, the four multilayers remain stable under physiological conditions, but are readily degraded in the presence of reducing agents. Higher concentrations of reducing agent degraded the films faster as shown by the use of 0.4 and 10 mM glutathione. It is interesting to note that at 2.5 mM, DTT shows a higher reducing rate than 10 mM glutathione for ChS-based films, but lower for PVA-based films. Glutathione has an extra negative charge due to the presence of glutamic acid in this molecule. It is therefore more negative than DTT and thus may experience more electrostatic repulsion in ChS films. This does not play a role in PVA films. Moreover, from both the UV spectroscopy and thickness characterizations, it is likely that in addition to having much higher thickness, the ChS-based films also contain higher portion of ChS in comparison to the degradable BA-PAAs and therefore, diffusion of reducing agents into the inner part of the film may be slightly impeded relative to the thinner PVA multilayers. This may further explain the high standard deviation observed for the ChS systems.Fig. 9


Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s.

Hujaya SD, Engbersen JF, Paulusse JM - Pharm. Res. (2015)

Stability profiles of: (a) PVA- and (b) ChS-based multilayers under physiological conditions (PBS pH 7.4 at 37°C) and in the presence of glutathione (0.4 and 10 mM) or DTT (2.5 mM). The left part of the abscissa shows the trend of fast decrease (burst release) within 0.5 h (30 min) in the initial phase of the incubation while the right part shows the much slower decrease throughout the rest of the long incubation duration.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4526598&req=5

Fig9: Stability profiles of: (a) PVA- and (b) ChS-based multilayers under physiological conditions (PBS pH 7.4 at 37°C) and in the presence of glutathione (0.4 and 10 mM) or DTT (2.5 mM). The left part of the abscissa shows the trend of fast decrease (burst release) within 0.5 h (30 min) in the initial phase of the incubation while the right part shows the much slower decrease throughout the rest of the long incubation duration.
Mentions: Figure 9 shows the decrease in relative absorbance values in time, i.e., the ratio of absorbance value against the respective value at time zero. Figure 9a and b show the results for PVA- and ChS-based systems, respectively. Throughout the incubation time, the four multilayers remain stable under physiological conditions, but are readily degraded in the presence of reducing agents. Higher concentrations of reducing agent degraded the films faster as shown by the use of 0.4 and 10 mM glutathione. It is interesting to note that at 2.5 mM, DTT shows a higher reducing rate than 10 mM glutathione for ChS-based films, but lower for PVA-based films. Glutathione has an extra negative charge due to the presence of glutamic acid in this molecule. It is therefore more negative than DTT and thus may experience more electrostatic repulsion in ChS films. This does not play a role in PVA films. Moreover, from both the UV spectroscopy and thickness characterizations, it is likely that in addition to having much higher thickness, the ChS-based films also contain higher portion of ChS in comparison to the degradable BA-PAAs and therefore, diffusion of reducing agents into the inner part of the film may be slightly impeded relative to the thinner PVA multilayers. This may further explain the high standard deviation observed for the ChS systems.Fig. 9

Bottom Line: Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers.Graphical Abstract Layer-by-Layer Assembly.

View Article: PubMed Central - PubMed

Affiliation: Department of Controlled Drug Delivery, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands.

ABSTRACT

Purpose: To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells.

Methods: Copolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.

Results: PVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10-100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells.

Conclusions: Responsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants. Graphical Abstract Layer-by-Layer Assembly.

No MeSH data available.


Related in: MedlinePlus