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Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s.

Hujaya SD, Engbersen JF, Paulusse JM - Pharm. Res. (2015)

Bottom Line: Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers.Graphical Abstract Layer-by-Layer Assembly.

View Article: PubMed Central - PubMed

Affiliation: Department of Controlled Drug Delivery, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands.

ABSTRACT

Purpose: To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells.

Methods: Copolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.

Results: PVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10-100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells.

Conclusions: Responsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants. Graphical Abstract Layer-by-Layer Assembly.

No MeSH data available.


Related in: MedlinePlus

Digital photograph of ABOL-BA-PAA-(PVA#ABOL-BA-PAA) and ABOL-BA-PAA-(ChS#ABOL-BA-PAA) built on silicon wafer at various bilayer numbers.
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Fig8: Digital photograph of ABOL-BA-PAA-(PVA#ABOL-BA-PAA) and ABOL-BA-PAA-(ChS#ABOL-BA-PAA) built on silicon wafer at various bilayer numbers.

Mentions: In addition to providing thickness information, AFM was also used to gain insights into the possible differences in surface profiles of the different films, and at different bilayer numbers. A digital photograph of the complete set of ABOL-BA-PAA-based samples, both with PVA and ChS at various bilayer numbers is shown in Fig. 8. DAB-BA-PAA-based films look similar, but with less homogeneous surfaces and increased turbidity. Figure 8 shows the typical thin-film interference color pattern observed during build-up on silicon wafers of various multilayers reported in this thesis. Notably, films of ~100 nm thick show deep blue interference color as shown for ABOL-BA-PAA-(PVA#ABOL-BA-PAA)10 in Fig. 8. In agreement with the thickness data, ABOL-BA-PAA-(ChS#ABOL-BA-PAA) reaches the same thickness much earlier at 3.5 bl and shows the same deep blue color.Fig. 8


Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s.

Hujaya SD, Engbersen JF, Paulusse JM - Pharm. Res. (2015)

Digital photograph of ABOL-BA-PAA-(PVA#ABOL-BA-PAA) and ABOL-BA-PAA-(ChS#ABOL-BA-PAA) built on silicon wafer at various bilayer numbers.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4526598&req=5

Fig8: Digital photograph of ABOL-BA-PAA-(PVA#ABOL-BA-PAA) and ABOL-BA-PAA-(ChS#ABOL-BA-PAA) built on silicon wafer at various bilayer numbers.
Mentions: In addition to providing thickness information, AFM was also used to gain insights into the possible differences in surface profiles of the different films, and at different bilayer numbers. A digital photograph of the complete set of ABOL-BA-PAA-based samples, both with PVA and ChS at various bilayer numbers is shown in Fig. 8. DAB-BA-PAA-based films look similar, but with less homogeneous surfaces and increased turbidity. Figure 8 shows the typical thin-film interference color pattern observed during build-up on silicon wafers of various multilayers reported in this thesis. Notably, films of ~100 nm thick show deep blue interference color as shown for ABOL-BA-PAA-(PVA#ABOL-BA-PAA)10 in Fig. 8. In agreement with the thickness data, ABOL-BA-PAA-(ChS#ABOL-BA-PAA) reaches the same thickness much earlier at 3.5 bl and shows the same deep blue color.Fig. 8

Bottom Line: Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers.Graphical Abstract Layer-by-Layer Assembly.

View Article: PubMed Central - PubMed

Affiliation: Department of Controlled Drug Delivery, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands.

ABSTRACT

Purpose: To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells.

Methods: Copolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.

Results: PVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10-100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells.

Conclusions: Responsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants. Graphical Abstract Layer-by-Layer Assembly.

No MeSH data available.


Related in: MedlinePlus