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Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s.

Hujaya SD, Engbersen JF, Paulusse JM - Pharm. Res. (2015)

Bottom Line: Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers.Graphical Abstract Layer-by-Layer Assembly.

View Article: PubMed Central - PubMed

Affiliation: Department of Controlled Drug Delivery, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands.

ABSTRACT

Purpose: To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells.

Methods: Copolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.

Results: PVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10-100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells.

Conclusions: Responsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants. Graphical Abstract Layer-by-Layer Assembly.

No MeSH data available.


Related in: MedlinePlus

Build-up profiles of DAB-BA-PAA-(PVA#DAB-BA-PAA), DAB-BA-PAA-(ChS#DAB-BA-PAA), ABOL-BA-PAA-(PVA#ABOL-BA-PAA), and ABOL-BA-PAA-(ChS#ABOL-BA-PAA) multilayers. Absorbance values shown are those at 227 nm for PVA-based films and 258 nm for ChS-based films.
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Fig5: Build-up profiles of DAB-BA-PAA-(PVA#DAB-BA-PAA), DAB-BA-PAA-(ChS#DAB-BA-PAA), ABOL-BA-PAA-(PVA#ABOL-BA-PAA), and ABOL-BA-PAA-(ChS#ABOL-BA-PAA) multilayers. Absorbance values shown are those at 227 nm for PVA-based films and 258 nm for ChS-based films.

Mentions: Based on the respective absorption maxima, build-up profiles can be plotted for the multilayers. All of the BA-based systems exhibit relatively linear build-up profiles as shown in Fig. 5. The control polymer DAB-Bn-PAA, however, provides a remarkable finding. For this polymer which does not contain a BA moiety, multilayer build-up is positive when paired with ChS, but negative when paired with PVA. This phenomenon indicates that the positive multilayer build-up of PVA-based films in this study depends substantially on the boronate ester formation. This finding also implies that multilayer build-up with ChS may still progress without boronate ester formation.Fig. 5


Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s.

Hujaya SD, Engbersen JF, Paulusse JM - Pharm. Res. (2015)

Build-up profiles of DAB-BA-PAA-(PVA#DAB-BA-PAA), DAB-BA-PAA-(ChS#DAB-BA-PAA), ABOL-BA-PAA-(PVA#ABOL-BA-PAA), and ABOL-BA-PAA-(ChS#ABOL-BA-PAA) multilayers. Absorbance values shown are those at 227 nm for PVA-based films and 258 nm for ChS-based films.
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Related In: Results  -  Collection

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Fig5: Build-up profiles of DAB-BA-PAA-(PVA#DAB-BA-PAA), DAB-BA-PAA-(ChS#DAB-BA-PAA), ABOL-BA-PAA-(PVA#ABOL-BA-PAA), and ABOL-BA-PAA-(ChS#ABOL-BA-PAA) multilayers. Absorbance values shown are those at 227 nm for PVA-based films and 258 nm for ChS-based films.
Mentions: Based on the respective absorption maxima, build-up profiles can be plotted for the multilayers. All of the BA-based systems exhibit relatively linear build-up profiles as shown in Fig. 5. The control polymer DAB-Bn-PAA, however, provides a remarkable finding. For this polymer which does not contain a BA moiety, multilayer build-up is positive when paired with ChS, but negative when paired with PVA. This phenomenon indicates that the positive multilayer build-up of PVA-based films in this study depends substantially on the boronate ester formation. This finding also implies that multilayer build-up with ChS may still progress without boronate ester formation.Fig. 5

Bottom Line: Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers.Graphical Abstract Layer-by-Layer Assembly.

View Article: PubMed Central - PubMed

Affiliation: Department of Controlled Drug Delivery, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands.

ABSTRACT

Purpose: To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells.

Methods: Copolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.

Results: PVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10-100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells.

Conclusions: Responsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants. Graphical Abstract Layer-by-Layer Assembly.

No MeSH data available.


Related in: MedlinePlus