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Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s.

Hujaya SD, Engbersen JF, Paulusse JM - Pharm. Res. (2015)

Bottom Line: Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers.Graphical Abstract Layer-by-Layer Assembly.

View Article: PubMed Central - PubMed

Affiliation: Department of Controlled Drug Delivery, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands.

ABSTRACT

Purpose: To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells.

Methods: Copolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.

Results: PVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10-100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells.

Conclusions: Responsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants. Graphical Abstract Layer-by-Layer Assembly.

No MeSH data available.


Related in: MedlinePlus

UV spectra of DAB-BA-PAA-(ChS#DAB-BA-PAA) and DAB-BA-PAA-(PVA#DAB-BA-PAA) at various bilayer numbers.
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Fig3: UV spectra of DAB-BA-PAA-(ChS#DAB-BA-PAA) and DAB-BA-PAA-(PVA#DAB-BA-PAA) at various bilayer numbers.

Mentions: As indicated in the previous section, the study of build-up profiles and the relative incremental increase in material deposition was carried out through the UV spectra of the multilayers in the dry state which were recorded after every deposition cycle, starting from 1 bl (with BA-PAA layer on top). The UV spectra of the different multilayers were similar with respect to whether DAB-BA-PAA, ABOL-BA-PAA, or DAB-Bn-PAA was employed as the BA- or Bn-functionalized polymer. Therefore, as a representative example, the UV spectra of DAB-BA-PAA-based systems at increasing bilayer numbers are displayed in Fig. 3. Depending on whether ChS or PVA was incorporated, the UV spectra had maxima at 258 and 227 nm, respectively. On the ChS system, the maximum at 227 nm appears as a shoulder with much higher absorption that exceeds the ideal absorption of ≤ 1 A.U. for maintained linearity at higher bilayer numbers. Therefore the more reliable maximum at 258 nm is used for further study on the ChS system.Fig. 3


Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s.

Hujaya SD, Engbersen JF, Paulusse JM - Pharm. Res. (2015)

UV spectra of DAB-BA-PAA-(ChS#DAB-BA-PAA) and DAB-BA-PAA-(PVA#DAB-BA-PAA) at various bilayer numbers.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4526598&req=5

Fig3: UV spectra of DAB-BA-PAA-(ChS#DAB-BA-PAA) and DAB-BA-PAA-(PVA#DAB-BA-PAA) at various bilayer numbers.
Mentions: As indicated in the previous section, the study of build-up profiles and the relative incremental increase in material deposition was carried out through the UV spectra of the multilayers in the dry state which were recorded after every deposition cycle, starting from 1 bl (with BA-PAA layer on top). The UV spectra of the different multilayers were similar with respect to whether DAB-BA-PAA, ABOL-BA-PAA, or DAB-Bn-PAA was employed as the BA- or Bn-functionalized polymer. Therefore, as a representative example, the UV spectra of DAB-BA-PAA-based systems at increasing bilayer numbers are displayed in Fig. 3. Depending on whether ChS or PVA was incorporated, the UV spectra had maxima at 258 and 227 nm, respectively. On the ChS system, the maximum at 227 nm appears as a shoulder with much higher absorption that exceeds the ideal absorption of ≤ 1 A.U. for maintained linearity at higher bilayer numbers. Therefore the more reliable maximum at 258 nm is used for further study on the ChS system.Fig. 3

Bottom Line: Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers.Graphical Abstract Layer-by-Layer Assembly.

View Article: PubMed Central - PubMed

Affiliation: Department of Controlled Drug Delivery, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands.

ABSTRACT

Purpose: To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells.

Methods: Copolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.

Results: PVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10-100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells.

Conclusions: Responsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants. Graphical Abstract Layer-by-Layer Assembly.

No MeSH data available.


Related in: MedlinePlus