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Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s.

Hujaya SD, Engbersen JF, Paulusse JM - Pharm. Res. (2015)

Bottom Line: Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers.Graphical Abstract Layer-by-Layer Assembly.

View Article: PubMed Central - PubMed

Affiliation: Department of Controlled Drug Delivery, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands.

ABSTRACT

Purpose: To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells.

Methods: Copolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.

Results: PVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10-100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells.

Conclusions: Responsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants. Graphical Abstract Layer-by-Layer Assembly.

No MeSH data available.


Related in: MedlinePlus

Metabolic activity of COS-7 cells cultured on untreated PS and various multilayered surfaces relative to TCPS control. All values are not significantly different from one another (p > 0.05).
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Fig13: Metabolic activity of COS-7 cells cultured on untreated PS and various multilayered surfaces relative to TCPS control. All values are not significantly different from one another (p > 0.05).

Mentions: In agreement with the live/dead staining results shown in Fig. 12b, the alamarblue (AB) assay for metabolic activity (Fig. 13) indicates that there is no significant difference in the metabolic activity of cells cultured on all of the studied surfaces. As expected, the more charged TCPS provides the best cell proliferation through faster cell attachment, while untreated PS provides slower cell attachment leading to slightly less metabolic activity. On the multilayered systems, ChS-based surfaces facilitate the best cell attachment, morphology and metabolic activity. Such high biocompatibility is expected given the natural biological origin of ChS. PVA-systems were observed to initially facilitate cell attachment, which means that the surface performs well in adsorbing anchoring proteins to facilitate cell attachment (59,60). However, upon longer culture duration the cells start to tend to aggregate, probably indicating slight cytotoxic effect of the surface. Considering the generally accepted biocompatibility of PVA (61), the effect may be induced by interaction of the cells with the BA-PAAs. Nevertheless the effect is mild, as the cells still show metabolic activity comparable to all the other surfaces. It can therefore be concluded that all of the multilayered systems investigated in this study are biocompatible.Fig. 13


Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s.

Hujaya SD, Engbersen JF, Paulusse JM - Pharm. Res. (2015)

Metabolic activity of COS-7 cells cultured on untreated PS and various multilayered surfaces relative to TCPS control. All values are not significantly different from one another (p > 0.05).
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4526598&req=5

Fig13: Metabolic activity of COS-7 cells cultured on untreated PS and various multilayered surfaces relative to TCPS control. All values are not significantly different from one another (p > 0.05).
Mentions: In agreement with the live/dead staining results shown in Fig. 12b, the alamarblue (AB) assay for metabolic activity (Fig. 13) indicates that there is no significant difference in the metabolic activity of cells cultured on all of the studied surfaces. As expected, the more charged TCPS provides the best cell proliferation through faster cell attachment, while untreated PS provides slower cell attachment leading to slightly less metabolic activity. On the multilayered systems, ChS-based surfaces facilitate the best cell attachment, morphology and metabolic activity. Such high biocompatibility is expected given the natural biological origin of ChS. PVA-systems were observed to initially facilitate cell attachment, which means that the surface performs well in adsorbing anchoring proteins to facilitate cell attachment (59,60). However, upon longer culture duration the cells start to tend to aggregate, probably indicating slight cytotoxic effect of the surface. Considering the generally accepted biocompatibility of PVA (61), the effect may be induced by interaction of the cells with the BA-PAAs. Nevertheless the effect is mild, as the cells still show metabolic activity comparable to all the other surfaces. It can therefore be concluded that all of the multilayered systems investigated in this study are biocompatible.Fig. 13

Bottom Line: Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers.Graphical Abstract Layer-by-Layer Assembly.

View Article: PubMed Central - PubMed

Affiliation: Department of Controlled Drug Delivery, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands.

ABSTRACT

Purpose: To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells.

Methods: Copolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.

Results: PVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10-100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells.

Conclusions: Responsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants. Graphical Abstract Layer-by-Layer Assembly.

No MeSH data available.


Related in: MedlinePlus