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Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s.

Hujaya SD, Engbersen JF, Paulusse JM - Pharm. Res. (2015)

Bottom Line: Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers.Graphical Abstract Layer-by-Layer Assembly.

View Article: PubMed Central - PubMed

Affiliation: Department of Controlled Drug Delivery, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands.

ABSTRACT

Purpose: To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells.

Methods: Copolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.

Results: PVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10-100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells.

Conclusions: Responsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants. Graphical Abstract Layer-by-Layer Assembly.

No MeSH data available.


Related in: MedlinePlus

COS-7 (a) Light microscopy images of COS-7 cell after 6 h of culture on various surfaces. (b) Overlay fluorescence images following cell staining with Hoechst 33258 (blue, live) and EthD-1 (red, dead) after 2 days of culture on various surfaces. 10× magnification, bars = 400 μm.
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Fig12: COS-7 (a) Light microscopy images of COS-7 cell after 6 h of culture on various surfaces. (b) Overlay fluorescence images following cell staining with Hoechst 33258 (blue, live) and EthD-1 (red, dead) after 2 days of culture on various surfaces. 10× magnification, bars = 400 μm.

Mentions: In order to obtain a functionalized multilayered surface for biomedical applications, it is necessary to study the biocompatibility of the surface. For an in vitro experiment, the morphology and metabolic activity of cells cultured on the surface may serve as a preliminary indication. For this experiment, films were built directly on the surfaces of 96-well plates, and COS-7 cells were seeded directly on top of the films to emphasize the effect of the substrates on cell morphology and viability. At the end of the 2 days culture period, metabolic activity was measured. Figure 12a shows that at 6 h after cell seeding, all of the cells have attached. All of the COS-7 cells seeded on multilayers seem to have similar morphology to those seeded on regular commercial TCPS (tissue culture-treated polystyrene), i.e., much better than the morphology of the cells seeded on the non-tissue culture treated polystyrene (i.e., untreated PS, normally utilized for suspension culture). However, after 2 days of culture (Fig. 12b), COS-7 cells on PVA-based films are seen to aggregate, similar to those on untreated PS. The EthD-1 which stains for dead cells (red), however, shows no significant difference in the amount of dead cells. Therefore, to further confirm the results, metabolic activity assay was performed at the end of the 2 days culture experiment.Fig. 12


Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s.

Hujaya SD, Engbersen JF, Paulusse JM - Pharm. Res. (2015)

COS-7 (a) Light microscopy images of COS-7 cell after 6 h of culture on various surfaces. (b) Overlay fluorescence images following cell staining with Hoechst 33258 (blue, live) and EthD-1 (red, dead) after 2 days of culture on various surfaces. 10× magnification, bars = 400 μm.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4526598&req=5

Fig12: COS-7 (a) Light microscopy images of COS-7 cell after 6 h of culture on various surfaces. (b) Overlay fluorescence images following cell staining with Hoechst 33258 (blue, live) and EthD-1 (red, dead) after 2 days of culture on various surfaces. 10× magnification, bars = 400 μm.
Mentions: In order to obtain a functionalized multilayered surface for biomedical applications, it is necessary to study the biocompatibility of the surface. For an in vitro experiment, the morphology and metabolic activity of cells cultured on the surface may serve as a preliminary indication. For this experiment, films were built directly on the surfaces of 96-well plates, and COS-7 cells were seeded directly on top of the films to emphasize the effect of the substrates on cell morphology and viability. At the end of the 2 days culture period, metabolic activity was measured. Figure 12a shows that at 6 h after cell seeding, all of the cells have attached. All of the COS-7 cells seeded on multilayers seem to have similar morphology to those seeded on regular commercial TCPS (tissue culture-treated polystyrene), i.e., much better than the morphology of the cells seeded on the non-tissue culture treated polystyrene (i.e., untreated PS, normally utilized for suspension culture). However, after 2 days of culture (Fig. 12b), COS-7 cells on PVA-based films are seen to aggregate, similar to those on untreated PS. The EthD-1 which stains for dead cells (red), however, shows no significant difference in the amount of dead cells. Therefore, to further confirm the results, metabolic activity assay was performed at the end of the 2 days culture experiment.Fig. 12

Bottom Line: Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers.Graphical Abstract Layer-by-Layer Assembly.

View Article: PubMed Central - PubMed

Affiliation: Department of Controlled Drug Delivery, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands.

ABSTRACT

Purpose: To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells.

Methods: Copolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.

Results: PVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10-100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells.

Conclusions: Responsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants. Graphical Abstract Layer-by-Layer Assembly.

No MeSH data available.


Related in: MedlinePlus