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Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s.

Hujaya SD, Engbersen JF, Paulusse JM - Pharm. Res. (2015)

Bottom Line: Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers.Graphical Abstract Layer-by-Layer Assembly.

View Article: PubMed Central - PubMed

Affiliation: Department of Controlled Drug Delivery, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands.

ABSTRACT

Purpose: To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells.

Methods: Copolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.

Results: PVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10-100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells.

Conclusions: Responsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants. Graphical Abstract Layer-by-Layer Assembly.

No MeSH data available.


Related in: MedlinePlus

Stability profiles of: (a) PVA- and (b) ChS-based multilayers under physiological conditions (PBS pH 7.4 at 37°C) and in the presence of 10, 20, and 100 mM glucose. For ChS systems, only the 100 mM glucose data is shown for clarity.
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Fig11: Stability profiles of: (a) PVA- and (b) ChS-based multilayers under physiological conditions (PBS pH 7.4 at 37°C) and in the presence of 10, 20, and 100 mM glucose. For ChS systems, only the 100 mM glucose data is shown for clarity.

Mentions: As indicated in Scheme 1, boronate ester formation may be affected by the presence of other diol moieties, such as glucose, through competitive binding. To investigate whether the multilayered systems possess glucose-responsiveness, the multilayers were incubated at various glucose concentrations under physiological conditions. Figure 11a and b show the stability profiles of PVA and ChS systems, respectively. From this figure it can be observed that the PVA systems are more responsive to glucose compared to the ChS system, especially apparent at the highest glucose concentration (100 mM). (ChS#DAB-BA-PAA) films show similar profiles with and without the presence of 100 mM glucose, while (ChS#ABOL-BA-PAA) systems seem to be only slightly responsive to glucose at the same concentration. At lower concentration, the ChS-based films were progressively more stable or identical to their profiles under physiological conditions (data not shown). The low responsiveness of the ChS system to elevated glucose concentrations, despite the long incubation duration, may be due to the more electrostatic nature of the interactions, possibly further enhanced by the thickness of the film limiting diffusion of glucose. As this may indicate that the majority of BA-moieties in the multilayered film are free of ester formation with ChS, this system may be utilized as a depot for drugs which have affinity to the BA-moiety, thereby providing more controlled or triggered release. This possibility is currently under investigation.Fig. 11


Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s.

Hujaya SD, Engbersen JF, Paulusse JM - Pharm. Res. (2015)

Stability profiles of: (a) PVA- and (b) ChS-based multilayers under physiological conditions (PBS pH 7.4 at 37°C) and in the presence of 10, 20, and 100 mM glucose. For ChS systems, only the 100 mM glucose data is shown for clarity.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4526598&req=5

Fig11: Stability profiles of: (a) PVA- and (b) ChS-based multilayers under physiological conditions (PBS pH 7.4 at 37°C) and in the presence of 10, 20, and 100 mM glucose. For ChS systems, only the 100 mM glucose data is shown for clarity.
Mentions: As indicated in Scheme 1, boronate ester formation may be affected by the presence of other diol moieties, such as glucose, through competitive binding. To investigate whether the multilayered systems possess glucose-responsiveness, the multilayers were incubated at various glucose concentrations under physiological conditions. Figure 11a and b show the stability profiles of PVA and ChS systems, respectively. From this figure it can be observed that the PVA systems are more responsive to glucose compared to the ChS system, especially apparent at the highest glucose concentration (100 mM). (ChS#DAB-BA-PAA) films show similar profiles with and without the presence of 100 mM glucose, while (ChS#ABOL-BA-PAA) systems seem to be only slightly responsive to glucose at the same concentration. At lower concentration, the ChS-based films were progressively more stable or identical to their profiles under physiological conditions (data not shown). The low responsiveness of the ChS system to elevated glucose concentrations, despite the long incubation duration, may be due to the more electrostatic nature of the interactions, possibly further enhanced by the thickness of the film limiting diffusion of glucose. As this may indicate that the majority of BA-moieties in the multilayered film are free of ester formation with ChS, this system may be utilized as a depot for drugs which have affinity to the BA-moiety, thereby providing more controlled or triggered release. This possibility is currently under investigation.Fig. 11

Bottom Line: Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers.Graphical Abstract Layer-by-Layer Assembly.

View Article: PubMed Central - PubMed

Affiliation: Department of Controlled Drug Delivery, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands.

ABSTRACT

Purpose: To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells.

Methods: Copolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.

Results: PVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10-100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells.

Conclusions: Responsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants. Graphical Abstract Layer-by-Layer Assembly.

No MeSH data available.


Related in: MedlinePlus