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Promising upshot of silver nanoparticles primed from Gracilaria crassa against bacterial pathogens.

Lavakumar V, Masilamani K, Ravichandiran V, Venkateshan N, Saigopal DV, Ashok Kumar CK, Sowmya C - Chem Cent J (2015)

Bottom Line: Average size lays at 122.7 nm and zeta potential was found to be -34.9 mV.The activity was found to be sky-scraping in a dose dependent manner.This will assure the out put of superior antibacterial formulation for near future.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biotechnology, Sree Vidhyanikethan College of Pharmacy, A.Rangampet, Tirupati, 517102 AP India.

ABSTRACT

Background: The study on newer antimicrobial agent from metal based nano materials has augmented in recent years for the management of multidrug resistance microorganisms. In our present investigation, we synthesized silver nanoparticles (AgNP's) from red algae, Gracilaria crassa as beginning material which effectively condensed the silver ions to silver nanoparticles with less price tag and no risk.

Methods: Silver nanoparticles were prepared by simple reaction of 1 mM AgNO3 with G. crassa extracts at room temperature. The fabricated AgNP's were subjected for characterization and screened against various microorganisms for antibacterial activity.

Results: UV-Vis spectroscopy (200-800 nm), XRD, FESEM and EDAX, were performed for AgNP's. UV-Vis spectroscopy demonstrated the absorption edge at 443 nm and EDAX pattern is purely due to the particle size and face centered cubic (fcc) symmetry of nanoparticles. Average size lays at 122.7 nm and zeta potential was found to be -34.9 mV. The antibacterial outcome of synthesized AgNP's (at the dose of 20 and 40 µg/ml) was evaluated against Escherichia coli, Proteus mirabilis, Bacillus subtilis and Pseudomonas aeruginosa. The mechanism of synthesized AgNP's bactericidal bustle is discussed in terms of interaction with the cell membrane of bacteria. The activity was found to be sky-scraping in a dose dependent manner.

Conclusion: Thus, environmental friendly, cost effective, non hazardous stable nanoparticles were prepared by green synthesis using red algae, G. crassa. Synthesized G. crassa AgNP's were in acceptable size and shape. Further, it elicits better bactericidal activity against microorganism. This will assure the out put of superior antibacterial formulation for near future.

No MeSH data available.


Related in: MedlinePlus

Sensitivity prototypes of silver nanoparticles against various microbial pathogens.
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Fig9: Sensitivity prototypes of silver nanoparticles against various microbial pathogens.

Mentions: Antibacterial activity by agar well diffusion technique was recorded after 24 h incubation of culture plates. The AgNP’s demonstrate excellent antibacterial activity against all tested microorganisms (Fig. 8). AgNP’s showed high spectrum of activity against E. coli and P. mirabilis at the concentrations of 20 and 40 µg/ml when compared with standard (Fig. 9). The significant zone of inhibition was exerted due to effect of AgNP’s on biochemical process of the bacterial cell by interacting thiol and amino groups of proteins and nucleic acids of cell wall [37–39]. Further, this could lead to interaction between nanoparticles and microorganism which results in triggering the discharge of highly reactive oxygen species (ROS), mostly hydroxyl radicals and singlet oxygen [40–42]. This augments the deposition of nanoparticles on the bacterial cell surface results large accumulation of silver nanoparticles causing disruption of cellular functions.Fig. 8


Promising upshot of silver nanoparticles primed from Gracilaria crassa against bacterial pathogens.

Lavakumar V, Masilamani K, Ravichandiran V, Venkateshan N, Saigopal DV, Ashok Kumar CK, Sowmya C - Chem Cent J (2015)

Sensitivity prototypes of silver nanoparticles against various microbial pathogens.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526595&req=5

Fig9: Sensitivity prototypes of silver nanoparticles against various microbial pathogens.
Mentions: Antibacterial activity by agar well diffusion technique was recorded after 24 h incubation of culture plates. The AgNP’s demonstrate excellent antibacterial activity against all tested microorganisms (Fig. 8). AgNP’s showed high spectrum of activity against E. coli and P. mirabilis at the concentrations of 20 and 40 µg/ml when compared with standard (Fig. 9). The significant zone of inhibition was exerted due to effect of AgNP’s on biochemical process of the bacterial cell by interacting thiol and amino groups of proteins and nucleic acids of cell wall [37–39]. Further, this could lead to interaction between nanoparticles and microorganism which results in triggering the discharge of highly reactive oxygen species (ROS), mostly hydroxyl radicals and singlet oxygen [40–42]. This augments the deposition of nanoparticles on the bacterial cell surface results large accumulation of silver nanoparticles causing disruption of cellular functions.Fig. 8

Bottom Line: Average size lays at 122.7 nm and zeta potential was found to be -34.9 mV.The activity was found to be sky-scraping in a dose dependent manner.This will assure the out put of superior antibacterial formulation for near future.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biotechnology, Sree Vidhyanikethan College of Pharmacy, A.Rangampet, Tirupati, 517102 AP India.

ABSTRACT

Background: The study on newer antimicrobial agent from metal based nano materials has augmented in recent years for the management of multidrug resistance microorganisms. In our present investigation, we synthesized silver nanoparticles (AgNP's) from red algae, Gracilaria crassa as beginning material which effectively condensed the silver ions to silver nanoparticles with less price tag and no risk.

Methods: Silver nanoparticles were prepared by simple reaction of 1 mM AgNO3 with G. crassa extracts at room temperature. The fabricated AgNP's were subjected for characterization and screened against various microorganisms for antibacterial activity.

Results: UV-Vis spectroscopy (200-800 nm), XRD, FESEM and EDAX, were performed for AgNP's. UV-Vis spectroscopy demonstrated the absorption edge at 443 nm and EDAX pattern is purely due to the particle size and face centered cubic (fcc) symmetry of nanoparticles. Average size lays at 122.7 nm and zeta potential was found to be -34.9 mV. The antibacterial outcome of synthesized AgNP's (at the dose of 20 and 40 µg/ml) was evaluated against Escherichia coli, Proteus mirabilis, Bacillus subtilis and Pseudomonas aeruginosa. The mechanism of synthesized AgNP's bactericidal bustle is discussed in terms of interaction with the cell membrane of bacteria. The activity was found to be sky-scraping in a dose dependent manner.

Conclusion: Thus, environmental friendly, cost effective, non hazardous stable nanoparticles were prepared by green synthesis using red algae, G. crassa. Synthesized G. crassa AgNP's were in acceptable size and shape. Further, it elicits better bactericidal activity against microorganism. This will assure the out put of superior antibacterial formulation for near future.

No MeSH data available.


Related in: MedlinePlus