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Impaired Function of CD5+CD19+CD1dhi B10 Cells on IgE Secretion in an Atopic Dermatitis-Like Mouse Model.

Li J, Shen C, Liu Y, Li Y, Sun L, Jiao L, Jiao W, Xiao J, Shen C, Qi H, Xu F, Ma L - PLoS ONE (2015)

Bottom Line: Reports on the role of regulated cells in AD have recently evolved to regulate B cells, which may play a role in allergic inflammation as well.Moreover, no difference in the percentage of B10pro + B10 cells was observed between the AD and control groups.Altogether, these results suggest that the number of IL-10-producing B cells decreased in the AD group and these cells showed a defective regulatory function on IgE secretion.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Major Diseases in Children Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.

ABSTRACT
Atopic dermatitis (AD) is a chronic inflammatory pruritic skin disease in which the pathogenic mechanism is complicated and not completely understood. Reports on the role of regulated cells in AD have recently evolved to regulate B cells, which may play a role in allergic inflammation as well. In the present study, we examined the frequency and regulatory function of CD5+CD19+CD1dhi B10 cells in an AD-like mouse model. Our results showed that the percentage of CD5+CD19+CD1dhi B10 cells increased while the frequency of IL-10-producing B cells in CD19+B cells decreased in the mice of AD group. Moreover, no difference in the percentage of B10pro + B10 cells was observed between the AD and control groups. Strikingly, B10 cells from control mice effectively inhibited IgE secretion, whereas the suppressive function of B10 cells from the AD mice was significantly decreased, which was similar to that observed in the group without B10. Altogether, these results suggest that the number of IL-10-producing B cells decreased in the AD group and these cells showed a defective regulatory function on IgE secretion.

No MeSH data available.


Related in: MedlinePlus

Influence of CD5+CD19+CD1dhi B10 cells on IgE production.B10 cells were sorted from the spleen of AD mice (n = 5) and control mice (n = 5) by flow cytometry. The PBMCs from normal mice and B10 cells from control or AD mice were cultured with LPS (10 μg/mL), PMA (50 ng/mL), and iono (500 ng/mL) for 72h,then the IgElevels were determined in the supernatants by ELISA.PBMCs without B10 cell wereused as a negative control. No differences in IgE levels were observed in the AD and negative control groups, and both groups showed significantly higher IgElevel than that observed in the control group. Data are expressed as mean ± SEM. **P < 0.01, as analyzed by one-way ANOVA, followed by Tukey multiple-comparison test.
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pone.0132173.g003: Influence of CD5+CD19+CD1dhi B10 cells on IgE production.B10 cells were sorted from the spleen of AD mice (n = 5) and control mice (n = 5) by flow cytometry. The PBMCs from normal mice and B10 cells from control or AD mice were cultured with LPS (10 μg/mL), PMA (50 ng/mL), and iono (500 ng/mL) for 72h,then the IgElevels were determined in the supernatants by ELISA.PBMCs without B10 cell wereused as a negative control. No differences in IgE levels were observed in the AD and negative control groups, and both groups showed significantly higher IgElevel than that observed in the control group. Data are expressed as mean ± SEM. **P < 0.01, as analyzed by one-way ANOVA, followed by Tukey multiple-comparison test.

Mentions: AD is a chronic inflammatory skin disease that is characterized by an increase in serum IgE levels. To further identify the regulatory function of B10 cells in IgE antibody-producing cells, we sorted B10 cells from the spleen of mice. These experiments were performed on PBMCs. Purified B10 cells sorted from AD or control mice were cultured with PBMCs for 72 h and LPS+PMA+iono were added at the last 5 h. At the same time, PBMCs without B10 cells were used as negative control. No differences in IgE levels between PBMCs without B10 cells and PBMCs cultured with AD B10 cells was observed (Fig 3, P > 0.05). However, the IgE level of PBMCs cultured with control B10 cells was significantly lower than that observed in the other two groups (Fig 3, P < 0.01). These results indicated that B10 cells from control mice were capable of efficiently suppressing IgE production by PBMCs whereas dysfunctional of B10 cells from AD group on IgE secretion was observed.


Impaired Function of CD5+CD19+CD1dhi B10 Cells on IgE Secretion in an Atopic Dermatitis-Like Mouse Model.

Li J, Shen C, Liu Y, Li Y, Sun L, Jiao L, Jiao W, Xiao J, Shen C, Qi H, Xu F, Ma L - PLoS ONE (2015)

Influence of CD5+CD19+CD1dhi B10 cells on IgE production.B10 cells were sorted from the spleen of AD mice (n = 5) and control mice (n = 5) by flow cytometry. The PBMCs from normal mice and B10 cells from control or AD mice were cultured with LPS (10 μg/mL), PMA (50 ng/mL), and iono (500 ng/mL) for 72h,then the IgElevels were determined in the supernatants by ELISA.PBMCs without B10 cell wereused as a negative control. No differences in IgE levels were observed in the AD and negative control groups, and both groups showed significantly higher IgElevel than that observed in the control group. Data are expressed as mean ± SEM. **P < 0.01, as analyzed by one-way ANOVA, followed by Tukey multiple-comparison test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526574&req=5

pone.0132173.g003: Influence of CD5+CD19+CD1dhi B10 cells on IgE production.B10 cells were sorted from the spleen of AD mice (n = 5) and control mice (n = 5) by flow cytometry. The PBMCs from normal mice and B10 cells from control or AD mice were cultured with LPS (10 μg/mL), PMA (50 ng/mL), and iono (500 ng/mL) for 72h,then the IgElevels were determined in the supernatants by ELISA.PBMCs without B10 cell wereused as a negative control. No differences in IgE levels were observed in the AD and negative control groups, and both groups showed significantly higher IgElevel than that observed in the control group. Data are expressed as mean ± SEM. **P < 0.01, as analyzed by one-way ANOVA, followed by Tukey multiple-comparison test.
Mentions: AD is a chronic inflammatory skin disease that is characterized by an increase in serum IgE levels. To further identify the regulatory function of B10 cells in IgE antibody-producing cells, we sorted B10 cells from the spleen of mice. These experiments were performed on PBMCs. Purified B10 cells sorted from AD or control mice were cultured with PBMCs for 72 h and LPS+PMA+iono were added at the last 5 h. At the same time, PBMCs without B10 cells were used as negative control. No differences in IgE levels between PBMCs without B10 cells and PBMCs cultured with AD B10 cells was observed (Fig 3, P > 0.05). However, the IgE level of PBMCs cultured with control B10 cells was significantly lower than that observed in the other two groups (Fig 3, P < 0.01). These results indicated that B10 cells from control mice were capable of efficiently suppressing IgE production by PBMCs whereas dysfunctional of B10 cells from AD group on IgE secretion was observed.

Bottom Line: Reports on the role of regulated cells in AD have recently evolved to regulate B cells, which may play a role in allergic inflammation as well.Moreover, no difference in the percentage of B10pro + B10 cells was observed between the AD and control groups.Altogether, these results suggest that the number of IL-10-producing B cells decreased in the AD group and these cells showed a defective regulatory function on IgE secretion.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Major Diseases in Children Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.

ABSTRACT
Atopic dermatitis (AD) is a chronic inflammatory pruritic skin disease in which the pathogenic mechanism is complicated and not completely understood. Reports on the role of regulated cells in AD have recently evolved to regulate B cells, which may play a role in allergic inflammation as well. In the present study, we examined the frequency and regulatory function of CD5+CD19+CD1dhi B10 cells in an AD-like mouse model. Our results showed that the percentage of CD5+CD19+CD1dhi B10 cells increased while the frequency of IL-10-producing B cells in CD19+B cells decreased in the mice of AD group. Moreover, no difference in the percentage of B10pro + B10 cells was observed between the AD and control groups. Strikingly, B10 cells from control mice effectively inhibited IgE secretion, whereas the suppressive function of B10 cells from the AD mice was significantly decreased, which was similar to that observed in the group without B10. Altogether, these results suggest that the number of IL-10-producing B cells decreased in the AD group and these cells showed a defective regulatory function on IgE secretion.

No MeSH data available.


Related in: MedlinePlus