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Impaired Function of CD5+CD19+CD1dhi B10 Cells on IgE Secretion in an Atopic Dermatitis-Like Mouse Model.

Li J, Shen C, Liu Y, Li Y, Sun L, Jiao L, Jiao W, Xiao J, Shen C, Qi H, Xu F, Ma L - PLoS ONE (2015)

Bottom Line: Reports on the role of regulated cells in AD have recently evolved to regulate B cells, which may play a role in allergic inflammation as well.Moreover, no difference in the percentage of B10pro + B10 cells was observed between the AD and control groups.Altogether, these results suggest that the number of IL-10-producing B cells decreased in the AD group and these cells showed a defective regulatory function on IgE secretion.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Major Diseases in Children Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.

ABSTRACT
Atopic dermatitis (AD) is a chronic inflammatory pruritic skin disease in which the pathogenic mechanism is complicated and not completely understood. Reports on the role of regulated cells in AD have recently evolved to regulate B cells, which may play a role in allergic inflammation as well. In the present study, we examined the frequency and regulatory function of CD5+CD19+CD1dhi B10 cells in an AD-like mouse model. Our results showed that the percentage of CD5+CD19+CD1dhi B10 cells increased while the frequency of IL-10-producing B cells in CD19+B cells decreased in the mice of AD group. Moreover, no difference in the percentage of B10pro + B10 cells was observed between the AD and control groups. Strikingly, B10 cells from control mice effectively inhibited IgE secretion, whereas the suppressive function of B10 cells from the AD mice was significantly decreased, which was similar to that observed in the group without B10. Altogether, these results suggest that the number of IL-10-producing B cells decreased in the AD group and these cells showed a defective regulatory function on IgE secretion.

No MeSH data available.


Related in: MedlinePlus

Comparison of IL-10 production in CD19+ B cells of AD mice and control.The B10 cells in the spleen of control (n = 10) or AD mice (n = 10) were in vitro stimulated for 5h or 48h withLPS(10μg/mL),PMA (50ng/mL), iono(500 ng/mL),monensin (2 mM), and CD40(1μg/mL). The number of CD19+IL-10+B cells wasmeasured by flow cytometry by using intracellular cytokine staining. (A) Representative comparative phenotype of CD19+IL-10+B cells from a control and AD mouse at 5 h and 48 h of stimulation; (B) The mean (±SEM) frequency of CD19+ B cells in PBMCs of spleen between controland AD groups after cultured for 5 h; (C) The mean (± SEM) frequency of B10pro+B10 cells in PBMCs of spleen between control and AD groups after culturing for 48 h; Data are expressed as mean ± SEM. Data are expressed as mean ± SEM. **P < 0.01, as analyzed by one-way ANOVA, followed by Tukey multiple-comparison test.
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pone.0132173.g002: Comparison of IL-10 production in CD19+ B cells of AD mice and control.The B10 cells in the spleen of control (n = 10) or AD mice (n = 10) were in vitro stimulated for 5h or 48h withLPS(10μg/mL),PMA (50ng/mL), iono(500 ng/mL),monensin (2 mM), and CD40(1μg/mL). The number of CD19+IL-10+B cells wasmeasured by flow cytometry by using intracellular cytokine staining. (A) Representative comparative phenotype of CD19+IL-10+B cells from a control and AD mouse at 5 h and 48 h of stimulation; (B) The mean (±SEM) frequency of CD19+ B cells in PBMCs of spleen between controland AD groups after cultured for 5 h; (C) The mean (± SEM) frequency of B10pro+B10 cells in PBMCs of spleen between control and AD groups after culturing for 48 h; Data are expressed as mean ± SEM. Data are expressed as mean ± SEM. **P < 0.01, as analyzed by one-way ANOVA, followed by Tukey multiple-comparison test.

Mentions: To determine whether IL-10 production in CD5+CD19+CD1dhi B10 cells remained intact, spleen cells from AD or control mice were cultured with L+PIM [21]. L+PIM-stimulation for 5 h could induce IL-10 production of B10 cells. As shown in Fig 2, intracellular staining for IL-10 was observed after stimulation (Fig 2A). Although the percentage of IL-10-producing B cells in both groups significantly increased upon stimulation (data not shown), the percentage of IL-10-producing B cells in the AD group was lower than that observed in the controls (Fig 2B, P < 0.01). Combining the frequencies of CD5+CD19+CD1dhiB10 cells (Fig 1B), our results indicated that B10 cells from AD likely had an intrinsic defect that affected IL-10 expression after term stimulation.


Impaired Function of CD5+CD19+CD1dhi B10 Cells on IgE Secretion in an Atopic Dermatitis-Like Mouse Model.

Li J, Shen C, Liu Y, Li Y, Sun L, Jiao L, Jiao W, Xiao J, Shen C, Qi H, Xu F, Ma L - PLoS ONE (2015)

Comparison of IL-10 production in CD19+ B cells of AD mice and control.The B10 cells in the spleen of control (n = 10) or AD mice (n = 10) were in vitro stimulated for 5h or 48h withLPS(10μg/mL),PMA (50ng/mL), iono(500 ng/mL),monensin (2 mM), and CD40(1μg/mL). The number of CD19+IL-10+B cells wasmeasured by flow cytometry by using intracellular cytokine staining. (A) Representative comparative phenotype of CD19+IL-10+B cells from a control and AD mouse at 5 h and 48 h of stimulation; (B) The mean (±SEM) frequency of CD19+ B cells in PBMCs of spleen between controland AD groups after cultured for 5 h; (C) The mean (± SEM) frequency of B10pro+B10 cells in PBMCs of spleen between control and AD groups after culturing for 48 h; Data are expressed as mean ± SEM. Data are expressed as mean ± SEM. **P < 0.01, as analyzed by one-way ANOVA, followed by Tukey multiple-comparison test.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4526574&req=5

pone.0132173.g002: Comparison of IL-10 production in CD19+ B cells of AD mice and control.The B10 cells in the spleen of control (n = 10) or AD mice (n = 10) were in vitro stimulated for 5h or 48h withLPS(10μg/mL),PMA (50ng/mL), iono(500 ng/mL),monensin (2 mM), and CD40(1μg/mL). The number of CD19+IL-10+B cells wasmeasured by flow cytometry by using intracellular cytokine staining. (A) Representative comparative phenotype of CD19+IL-10+B cells from a control and AD mouse at 5 h and 48 h of stimulation; (B) The mean (±SEM) frequency of CD19+ B cells in PBMCs of spleen between controland AD groups after cultured for 5 h; (C) The mean (± SEM) frequency of B10pro+B10 cells in PBMCs of spleen between control and AD groups after culturing for 48 h; Data are expressed as mean ± SEM. Data are expressed as mean ± SEM. **P < 0.01, as analyzed by one-way ANOVA, followed by Tukey multiple-comparison test.
Mentions: To determine whether IL-10 production in CD5+CD19+CD1dhi B10 cells remained intact, spleen cells from AD or control mice were cultured with L+PIM [21]. L+PIM-stimulation for 5 h could induce IL-10 production of B10 cells. As shown in Fig 2, intracellular staining for IL-10 was observed after stimulation (Fig 2A). Although the percentage of IL-10-producing B cells in both groups significantly increased upon stimulation (data not shown), the percentage of IL-10-producing B cells in the AD group was lower than that observed in the controls (Fig 2B, P < 0.01). Combining the frequencies of CD5+CD19+CD1dhiB10 cells (Fig 1B), our results indicated that B10 cells from AD likely had an intrinsic defect that affected IL-10 expression after term stimulation.

Bottom Line: Reports on the role of regulated cells in AD have recently evolved to regulate B cells, which may play a role in allergic inflammation as well.Moreover, no difference in the percentage of B10pro + B10 cells was observed between the AD and control groups.Altogether, these results suggest that the number of IL-10-producing B cells decreased in the AD group and these cells showed a defective regulatory function on IgE secretion.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Major Diseases in Children Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.

ABSTRACT
Atopic dermatitis (AD) is a chronic inflammatory pruritic skin disease in which the pathogenic mechanism is complicated and not completely understood. Reports on the role of regulated cells in AD have recently evolved to regulate B cells, which may play a role in allergic inflammation as well. In the present study, we examined the frequency and regulatory function of CD5+CD19+CD1dhi B10 cells in an AD-like mouse model. Our results showed that the percentage of CD5+CD19+CD1dhi B10 cells increased while the frequency of IL-10-producing B cells in CD19+B cells decreased in the mice of AD group. Moreover, no difference in the percentage of B10pro + B10 cells was observed between the AD and control groups. Strikingly, B10 cells from control mice effectively inhibited IgE secretion, whereas the suppressive function of B10 cells from the AD mice was significantly decreased, which was similar to that observed in the group without B10. Altogether, these results suggest that the number of IL-10-producing B cells decreased in the AD group and these cells showed a defective regulatory function on IgE secretion.

No MeSH data available.


Related in: MedlinePlus