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Impaired Function of CD5+CD19+CD1dhi B10 Cells on IgE Secretion in an Atopic Dermatitis-Like Mouse Model.

Li J, Shen C, Liu Y, Li Y, Sun L, Jiao L, Jiao W, Xiao J, Shen C, Qi H, Xu F, Ma L - PLoS ONE (2015)

Bottom Line: Reports on the role of regulated cells in AD have recently evolved to regulate B cells, which may play a role in allergic inflammation as well.Moreover, no difference in the percentage of B10pro + B10 cells was observed between the AD and control groups.Altogether, these results suggest that the number of IL-10-producing B cells decreased in the AD group and these cells showed a defective regulatory function on IgE secretion.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Major Diseases in Children Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.

ABSTRACT
Atopic dermatitis (AD) is a chronic inflammatory pruritic skin disease in which the pathogenic mechanism is complicated and not completely understood. Reports on the role of regulated cells in AD have recently evolved to regulate B cells, which may play a role in allergic inflammation as well. In the present study, we examined the frequency and regulatory function of CD5+CD19+CD1dhi B10 cells in an AD-like mouse model. Our results showed that the percentage of CD5+CD19+CD1dhi B10 cells increased while the frequency of IL-10-producing B cells in CD19+B cells decreased in the mice of AD group. Moreover, no difference in the percentage of B10pro + B10 cells was observed between the AD and control groups. Strikingly, B10 cells from control mice effectively inhibited IgE secretion, whereas the suppressive function of B10 cells from the AD mice was significantly decreased, which was similar to that observed in the group without B10. Altogether, these results suggest that the number of IL-10-producing B cells decreased in the AD group and these cells showed a defective regulatory function on IgE secretion.

No MeSH data available.


Related in: MedlinePlus

Regulatory B10 cells are augmented and the level of IL-10 expression is decreased in the spleen of AD mice.(A) Representative dot-plots showing the percentage of CD5+CD19+CD1dhi B10 cells in control (n = 10) and AD mice (n = 10); (B) Bar graph depicting the percentage of CD5+CD19+CD1dhiB10 cells in control and AD mice as analyzed by flow cytometry. The percentage of CD5+CD19+CD1dhi B10 cells was significantly higher in the AD group than that observed in the control. Data are expressed as mean ± SEM.*P<0.05, asanalyzed by one-way ANOVA, followed by Tukey multiple-comparison test; (B) The level ofIL-10 mRNA expression decreased in the spleen of AD mice (n = 10) compared to that of control (n = 10). Data are expressed as mean ± SEM. *P < 0.05, as analyzed by one-way ANOVA, followed by Tukey multiple-comparison test.
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pone.0132173.g001: Regulatory B10 cells are augmented and the level of IL-10 expression is decreased in the spleen of AD mice.(A) Representative dot-plots showing the percentage of CD5+CD19+CD1dhi B10 cells in control (n = 10) and AD mice (n = 10); (B) Bar graph depicting the percentage of CD5+CD19+CD1dhiB10 cells in control and AD mice as analyzed by flow cytometry. The percentage of CD5+CD19+CD1dhi B10 cells was significantly higher in the AD group than that observed in the control. Data are expressed as mean ± SEM.*P<0.05, asanalyzed by one-way ANOVA, followed by Tukey multiple-comparison test; (B) The level ofIL-10 mRNA expression decreased in the spleen of AD mice (n = 10) compared to that of control (n = 10). Data are expressed as mean ± SEM. *P < 0.05, as analyzed by one-way ANOVA, followed by Tukey multiple-comparison test.

Mentions: To determine whether regulatory B10 cells are relevant to AD, the well-described model of AD was used in the present study. DNFB was repeatedly painted onto the backs of each mouse. Two days after the last sensitization, the frequency of CD5+CD19+CD1dhi B10 cells in the spleen was analyzed. As shown in Fig 1A and 1B, the percentage of CD5+CD19+CD1dhi B10 cells was significantly higher in the AD group compared to that observed in the controls (P < 0.05).


Impaired Function of CD5+CD19+CD1dhi B10 Cells on IgE Secretion in an Atopic Dermatitis-Like Mouse Model.

Li J, Shen C, Liu Y, Li Y, Sun L, Jiao L, Jiao W, Xiao J, Shen C, Qi H, Xu F, Ma L - PLoS ONE (2015)

Regulatory B10 cells are augmented and the level of IL-10 expression is decreased in the spleen of AD mice.(A) Representative dot-plots showing the percentage of CD5+CD19+CD1dhi B10 cells in control (n = 10) and AD mice (n = 10); (B) Bar graph depicting the percentage of CD5+CD19+CD1dhiB10 cells in control and AD mice as analyzed by flow cytometry. The percentage of CD5+CD19+CD1dhi B10 cells was significantly higher in the AD group than that observed in the control. Data are expressed as mean ± SEM.*P<0.05, asanalyzed by one-way ANOVA, followed by Tukey multiple-comparison test; (B) The level ofIL-10 mRNA expression decreased in the spleen of AD mice (n = 10) compared to that of control (n = 10). Data are expressed as mean ± SEM. *P < 0.05, as analyzed by one-way ANOVA, followed by Tukey multiple-comparison test.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4526574&req=5

pone.0132173.g001: Regulatory B10 cells are augmented and the level of IL-10 expression is decreased in the spleen of AD mice.(A) Representative dot-plots showing the percentage of CD5+CD19+CD1dhi B10 cells in control (n = 10) and AD mice (n = 10); (B) Bar graph depicting the percentage of CD5+CD19+CD1dhiB10 cells in control and AD mice as analyzed by flow cytometry. The percentage of CD5+CD19+CD1dhi B10 cells was significantly higher in the AD group than that observed in the control. Data are expressed as mean ± SEM.*P<0.05, asanalyzed by one-way ANOVA, followed by Tukey multiple-comparison test; (B) The level ofIL-10 mRNA expression decreased in the spleen of AD mice (n = 10) compared to that of control (n = 10). Data are expressed as mean ± SEM. *P < 0.05, as analyzed by one-way ANOVA, followed by Tukey multiple-comparison test.
Mentions: To determine whether regulatory B10 cells are relevant to AD, the well-described model of AD was used in the present study. DNFB was repeatedly painted onto the backs of each mouse. Two days after the last sensitization, the frequency of CD5+CD19+CD1dhi B10 cells in the spleen was analyzed. As shown in Fig 1A and 1B, the percentage of CD5+CD19+CD1dhi B10 cells was significantly higher in the AD group compared to that observed in the controls (P < 0.05).

Bottom Line: Reports on the role of regulated cells in AD have recently evolved to regulate B cells, which may play a role in allergic inflammation as well.Moreover, no difference in the percentage of B10pro + B10 cells was observed between the AD and control groups.Altogether, these results suggest that the number of IL-10-producing B cells decreased in the AD group and these cells showed a defective regulatory function on IgE secretion.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Major Diseases in Children Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.

ABSTRACT
Atopic dermatitis (AD) is a chronic inflammatory pruritic skin disease in which the pathogenic mechanism is complicated and not completely understood. Reports on the role of regulated cells in AD have recently evolved to regulate B cells, which may play a role in allergic inflammation as well. In the present study, we examined the frequency and regulatory function of CD5+CD19+CD1dhi B10 cells in an AD-like mouse model. Our results showed that the percentage of CD5+CD19+CD1dhi B10 cells increased while the frequency of IL-10-producing B cells in CD19+B cells decreased in the mice of AD group. Moreover, no difference in the percentage of B10pro + B10 cells was observed between the AD and control groups. Strikingly, B10 cells from control mice effectively inhibited IgE secretion, whereas the suppressive function of B10 cells from the AD mice was significantly decreased, which was similar to that observed in the group without B10. Altogether, these results suggest that the number of IL-10-producing B cells decreased in the AD group and these cells showed a defective regulatory function on IgE secretion.

No MeSH data available.


Related in: MedlinePlus