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Open-Source Photometric System for Enzymatic Nitrate Quantification.

Wittbrodt BT, Squires DA, Walbeck J, Campbell E, Campbell WH, Pearce JM - PLoS ONE (2015)

Bottom Line: To accomplish this goal, a methodology for the design, development, and technical validation of an improved open-source water testing platform capable of performing Nitrate Reductase Nitrate-Nitrogen Analysis Method.This approach is evaluated for its potential to i) eliminate the need for toxic chemicals in water testing for nitrate and nitrite, ii) reduce the cost of equipment to perform this method for measurement for water quality, and iii) make the method easier to carryout in the field.This allows for greater access to the technology and the new, safer nitrate testing technique.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Science & Engineering, Michigan Technological University, Houghton, MI, United States of America.

ABSTRACT
Nitrate, the most oxidized form of nitrogen, is regulated to protect people and animals from harmful levels as there is a large over abundance due to anthropogenic factors. Widespread field testing for nitrate could begin to address the nitrate pollution problem, however, the Cadmium Reduction Method, the leading certified method to detect and quantify nitrate, demands the use of a toxic heavy metal. An alternative, the recently proposed Environmental Protection Agency Nitrate Reductase Nitrate-Nitrogen Analysis Method, eliminates this problem but requires an expensive proprietary spectrophotometer. The development of an inexpensive portable, handheld photometer will greatly expedite field nitrate analysis to combat pollution. To accomplish this goal, a methodology for the design, development, and technical validation of an improved open-source water testing platform capable of performing Nitrate Reductase Nitrate-Nitrogen Analysis Method. This approach is evaluated for its potential to i) eliminate the need for toxic chemicals in water testing for nitrate and nitrite, ii) reduce the cost of equipment to perform this method for measurement for water quality, and iii) make the method easier to carryout in the field. The device is able to perform as well as commercial proprietary systems for less than 15% of the cost for materials. This allows for greater access to the technology and the new, safer nitrate testing technique.

No MeSH data available.


Nitrate Standard Curve used for calibration of the open-source photometer.
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pone.0134989.g008: Nitrate Standard Curve used for calibration of the open-source photometer.

Mentions: Fig 8 illustrates the relationship between concentration of nitrate-N (ppm) and the absorbance of the light within the sample when analyzed at 540 nm in an HP 8452a Diode Array Spectrophotometer. In this graphic, each point represents the mean of 5 independent standards for that nitrate concentration. The size of the symbol indicates the standard deviation of the mean for n = 5, which were all less than 10% relative standard deviation. A calibration curve for the sample is created from this data and its linear regression fit is programed into the software app for the photometer. The equation of the linear regression fitted line is shown in the box in Fig 8, which has a correlation coefficient = 0.999. The same nitrate standards were analyzed with the photometer and the inset graph in Fig 8 shows the results for the mean of 5 independent standards for each nitrate concentration plotted against the nominal concentration of the Nitrate standards. Again the size of the symbol shows the standard deviation of the photometer readings with none being greater than 10% of the mean. A line of equal equivalence is shown in the inset graph, where photometer reading would equal the nominal nitrate standard concentration.


Open-Source Photometric System for Enzymatic Nitrate Quantification.

Wittbrodt BT, Squires DA, Walbeck J, Campbell E, Campbell WH, Pearce JM - PLoS ONE (2015)

Nitrate Standard Curve used for calibration of the open-source photometer.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526554&req=5

pone.0134989.g008: Nitrate Standard Curve used for calibration of the open-source photometer.
Mentions: Fig 8 illustrates the relationship between concentration of nitrate-N (ppm) and the absorbance of the light within the sample when analyzed at 540 nm in an HP 8452a Diode Array Spectrophotometer. In this graphic, each point represents the mean of 5 independent standards for that nitrate concentration. The size of the symbol indicates the standard deviation of the mean for n = 5, which were all less than 10% relative standard deviation. A calibration curve for the sample is created from this data and its linear regression fit is programed into the software app for the photometer. The equation of the linear regression fitted line is shown in the box in Fig 8, which has a correlation coefficient = 0.999. The same nitrate standards were analyzed with the photometer and the inset graph in Fig 8 shows the results for the mean of 5 independent standards for each nitrate concentration plotted against the nominal concentration of the Nitrate standards. Again the size of the symbol shows the standard deviation of the photometer readings with none being greater than 10% of the mean. A line of equal equivalence is shown in the inset graph, where photometer reading would equal the nominal nitrate standard concentration.

Bottom Line: To accomplish this goal, a methodology for the design, development, and technical validation of an improved open-source water testing platform capable of performing Nitrate Reductase Nitrate-Nitrogen Analysis Method.This approach is evaluated for its potential to i) eliminate the need for toxic chemicals in water testing for nitrate and nitrite, ii) reduce the cost of equipment to perform this method for measurement for water quality, and iii) make the method easier to carryout in the field.This allows for greater access to the technology and the new, safer nitrate testing technique.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Science & Engineering, Michigan Technological University, Houghton, MI, United States of America.

ABSTRACT
Nitrate, the most oxidized form of nitrogen, is regulated to protect people and animals from harmful levels as there is a large over abundance due to anthropogenic factors. Widespread field testing for nitrate could begin to address the nitrate pollution problem, however, the Cadmium Reduction Method, the leading certified method to detect and quantify nitrate, demands the use of a toxic heavy metal. An alternative, the recently proposed Environmental Protection Agency Nitrate Reductase Nitrate-Nitrogen Analysis Method, eliminates this problem but requires an expensive proprietary spectrophotometer. The development of an inexpensive portable, handheld photometer will greatly expedite field nitrate analysis to combat pollution. To accomplish this goal, a methodology for the design, development, and technical validation of an improved open-source water testing platform capable of performing Nitrate Reductase Nitrate-Nitrogen Analysis Method. This approach is evaluated for its potential to i) eliminate the need for toxic chemicals in water testing for nitrate and nitrite, ii) reduce the cost of equipment to perform this method for measurement for water quality, and iii) make the method easier to carryout in the field. The device is able to perform as well as commercial proprietary systems for less than 15% of the cost for materials. This allows for greater access to the technology and the new, safer nitrate testing technique.

No MeSH data available.