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Open-Source Photometric System for Enzymatic Nitrate Quantification.

Wittbrodt BT, Squires DA, Walbeck J, Campbell E, Campbell WH, Pearce JM - PLoS ONE (2015)

Bottom Line: To accomplish this goal, a methodology for the design, development, and technical validation of an improved open-source water testing platform capable of performing Nitrate Reductase Nitrate-Nitrogen Analysis Method.This approach is evaluated for its potential to i) eliminate the need for toxic chemicals in water testing for nitrate and nitrite, ii) reduce the cost of equipment to perform this method for measurement for water quality, and iii) make the method easier to carryout in the field.This allows for greater access to the technology and the new, safer nitrate testing technique.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Science & Engineering, Michigan Technological University, Houghton, MI, United States of America.

ABSTRACT
Nitrate, the most oxidized form of nitrogen, is regulated to protect people and animals from harmful levels as there is a large over abundance due to anthropogenic factors. Widespread field testing for nitrate could begin to address the nitrate pollution problem, however, the Cadmium Reduction Method, the leading certified method to detect and quantify nitrate, demands the use of a toxic heavy metal. An alternative, the recently proposed Environmental Protection Agency Nitrate Reductase Nitrate-Nitrogen Analysis Method, eliminates this problem but requires an expensive proprietary spectrophotometer. The development of an inexpensive portable, handheld photometer will greatly expedite field nitrate analysis to combat pollution. To accomplish this goal, a methodology for the design, development, and technical validation of an improved open-source water testing platform capable of performing Nitrate Reductase Nitrate-Nitrogen Analysis Method. This approach is evaluated for its potential to i) eliminate the need for toxic chemicals in water testing for nitrate and nitrite, ii) reduce the cost of equipment to perform this method for measurement for water quality, and iii) make the method easier to carryout in the field. The device is able to perform as well as commercial proprietary systems for less than 15% of the cost for materials. This allows for greater access to the technology and the new, safer nitrate testing technique.

No MeSH data available.


Schematic for 1) the sensor board and 2) LED board.
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pone.0134989.g002: Schematic for 1) the sensor board and 2) LED board.

Mentions: The open-source electronics for the device are based off of the open-source Arduino microcontroller [38] using an inexpensive ATMega 168 chip and have been designed to communicate over Bluetooth to the user’s mobile device using the Roving Networks RN-42 Bluetooth module [39]. Power is supplied to the device with a 500 mAh lithium ion battery with the voltage stepped down from 3.7V to 3.3V [40]. Fig 1 shows the electronic schematic for the main ATMega board, the Bluetooth module, and the electric power and Fig 2 shows the electronic schematics for the sensors and LED array, respectively.


Open-Source Photometric System for Enzymatic Nitrate Quantification.

Wittbrodt BT, Squires DA, Walbeck J, Campbell E, Campbell WH, Pearce JM - PLoS ONE (2015)

Schematic for 1) the sensor board and 2) LED board.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526554&req=5

pone.0134989.g002: Schematic for 1) the sensor board and 2) LED board.
Mentions: The open-source electronics for the device are based off of the open-source Arduino microcontroller [38] using an inexpensive ATMega 168 chip and have been designed to communicate over Bluetooth to the user’s mobile device using the Roving Networks RN-42 Bluetooth module [39]. Power is supplied to the device with a 500 mAh lithium ion battery with the voltage stepped down from 3.7V to 3.3V [40]. Fig 1 shows the electronic schematic for the main ATMega board, the Bluetooth module, and the electric power and Fig 2 shows the electronic schematics for the sensors and LED array, respectively.

Bottom Line: To accomplish this goal, a methodology for the design, development, and technical validation of an improved open-source water testing platform capable of performing Nitrate Reductase Nitrate-Nitrogen Analysis Method.This approach is evaluated for its potential to i) eliminate the need for toxic chemicals in water testing for nitrate and nitrite, ii) reduce the cost of equipment to perform this method for measurement for water quality, and iii) make the method easier to carryout in the field.This allows for greater access to the technology and the new, safer nitrate testing technique.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Science & Engineering, Michigan Technological University, Houghton, MI, United States of America.

ABSTRACT
Nitrate, the most oxidized form of nitrogen, is regulated to protect people and animals from harmful levels as there is a large over abundance due to anthropogenic factors. Widespread field testing for nitrate could begin to address the nitrate pollution problem, however, the Cadmium Reduction Method, the leading certified method to detect and quantify nitrate, demands the use of a toxic heavy metal. An alternative, the recently proposed Environmental Protection Agency Nitrate Reductase Nitrate-Nitrogen Analysis Method, eliminates this problem but requires an expensive proprietary spectrophotometer. The development of an inexpensive portable, handheld photometer will greatly expedite field nitrate analysis to combat pollution. To accomplish this goal, a methodology for the design, development, and technical validation of an improved open-source water testing platform capable of performing Nitrate Reductase Nitrate-Nitrogen Analysis Method. This approach is evaluated for its potential to i) eliminate the need for toxic chemicals in water testing for nitrate and nitrite, ii) reduce the cost of equipment to perform this method for measurement for water quality, and iii) make the method easier to carryout in the field. The device is able to perform as well as commercial proprietary systems for less than 15% of the cost for materials. This allows for greater access to the technology and the new, safer nitrate testing technique.

No MeSH data available.