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Genotoxic Evaluation of Mexican Welders Occupationally Exposed to Welding-Fumes Using the Micronucleus Test on Exfoliated Oral Mucosa Cells: A Cross-Sectional, Case-Control Study.

Jara-Ettinger AC, López-Tavera JC, Zavala-Cerna MG, Torres-Bugarín O - PLoS ONE (2015)

Bottom Line: Previous studies show that the exposure to such fumes is associated with damage to genetic material and increased cancer risk.After adjusting for smoking, the regression results showed that the occurrence of binucleated cells could be predicted by the exposure to welding-fumes plus the presence of tobacco consumption; for the condensed-chromatin cells, our model showed that the exposure to welding-fumes is the only reliable predictor.Further studies should shed more light on this subject.

View Article: PubMed Central - PubMed

Affiliation: Faculty of medicine, Universidad Autónoma de Guadalajara, Zapopan, Jalisco, Mexico.

ABSTRACT

Background: An estimated 800,000 people worldwide are occupationally exposed to welding-fumes. Previous studies show that the exposure to such fumes is associated with damage to genetic material and increased cancer risk. In this study, we evaluate the genotoxic effect of welding-fumes using the Micronucleus Test on oral mucosa cells of Mexican welders.

Material and methods: We conducted a cross-sectional, matched case-control study of n = 66 (33 exposed welders, and 33 healthy controls). Buccal mucosa smears were collected and stained with acridine orange, observed under 100x optical amplification with a fluorescence lamp, and a single-blinded observer counted the number of micronuclei and other nuclear abnormalities per 2,000 observed cells. We compared the frequencies of micronuclei and other nuclear abnormalities, and fitted generalised linear models to investigate the interactions between nuclear abnormalities and the exposure to welding-fumes, while controlling for smoking and age.

Results: Binucleated cells and condensed-chromatin cells showed statistically significant differences between cases and controls. The frequency of micronuclei and the rest of nuclear abnormalities (lobed-nuclei, pyknosis, karyolysis, and karyorrhexis) did not differ significantly between the groups. After adjusting for smoking, the regression results showed that the occurrence of binucleated cells could be predicted by the exposure to welding-fumes plus the presence of tobacco consumption; for the condensed-chromatin cells, our model showed that the exposure to welding-fumes is the only reliable predictor.

Conclusions: Our findings suggest that Mexican welders who are occupationally exposed to welding-fumes have increased counts of binucleated and condensed-chromatin cells. Nevertheless, the frequencies of micronuclei and the rest of nuclear abnormalities did not differ between cases and controls. Further studies should shed more light on this subject.

No MeSH data available.


Related in: MedlinePlus

Microphotographs of MNi and NA, identified according to the HUMNxl scoring criteria.The figure shows a series of 8 microphotographs of oral mucosa cells stained with acridine orange at100x optic amplification with a Carl Zeiss IVFL Axiostar Plus microscope, 450–490 nm fluorescence filters. (A) Abnormal buccal cell without any MNi or NA, (B) A buccal cell with the presence of micronucleus(white arrow), (C) a lobed-nuclei cell, (D) a binucleated cell, (E) a pyknotic cell, (F) a condensed-chromatin cell, (G) a karyolytic cell karyorrhectic cell, and (H) karyorrhectic cell.
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pone.0131548.g001: Microphotographs of MNi and NA, identified according to the HUMNxl scoring criteria.The figure shows a series of 8 microphotographs of oral mucosa cells stained with acridine orange at100x optic amplification with a Carl Zeiss IVFL Axiostar Plus microscope, 450–490 nm fluorescence filters. (A) Abnormal buccal cell without any MNi or NA, (B) A buccal cell with the presence of micronucleus(white arrow), (C) a lobed-nuclei cell, (D) a binucleated cell, (E) a pyknotic cell, (F) a condensed-chromatin cell, (G) a karyolytic cell karyorrhectic cell, and (H) karyorrhectic cell.

Mentions: An experienced reader, blind to the group to which samples belonged, observed the slides under 100x optic amplification with a Carl Zeiss IVFL Axiostar Plus microscope equipped with 450–490 nm fluorescence filters. Using the HUMNxl scoring criteria [35], she identified and counted the number of MNi and NA occurrences per 2000 observed cells per slide (Fig 1).


Genotoxic Evaluation of Mexican Welders Occupationally Exposed to Welding-Fumes Using the Micronucleus Test on Exfoliated Oral Mucosa Cells: A Cross-Sectional, Case-Control Study.

Jara-Ettinger AC, López-Tavera JC, Zavala-Cerna MG, Torres-Bugarín O - PLoS ONE (2015)

Microphotographs of MNi and NA, identified according to the HUMNxl scoring criteria.The figure shows a series of 8 microphotographs of oral mucosa cells stained with acridine orange at100x optic amplification with a Carl Zeiss IVFL Axiostar Plus microscope, 450–490 nm fluorescence filters. (A) Abnormal buccal cell without any MNi or NA, (B) A buccal cell with the presence of micronucleus(white arrow), (C) a lobed-nuclei cell, (D) a binucleated cell, (E) a pyknotic cell, (F) a condensed-chromatin cell, (G) a karyolytic cell karyorrhectic cell, and (H) karyorrhectic cell.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526553&req=5

pone.0131548.g001: Microphotographs of MNi and NA, identified according to the HUMNxl scoring criteria.The figure shows a series of 8 microphotographs of oral mucosa cells stained with acridine orange at100x optic amplification with a Carl Zeiss IVFL Axiostar Plus microscope, 450–490 nm fluorescence filters. (A) Abnormal buccal cell without any MNi or NA, (B) A buccal cell with the presence of micronucleus(white arrow), (C) a lobed-nuclei cell, (D) a binucleated cell, (E) a pyknotic cell, (F) a condensed-chromatin cell, (G) a karyolytic cell karyorrhectic cell, and (H) karyorrhectic cell.
Mentions: An experienced reader, blind to the group to which samples belonged, observed the slides under 100x optic amplification with a Carl Zeiss IVFL Axiostar Plus microscope equipped with 450–490 nm fluorescence filters. Using the HUMNxl scoring criteria [35], she identified and counted the number of MNi and NA occurrences per 2000 observed cells per slide (Fig 1).

Bottom Line: Previous studies show that the exposure to such fumes is associated with damage to genetic material and increased cancer risk.After adjusting for smoking, the regression results showed that the occurrence of binucleated cells could be predicted by the exposure to welding-fumes plus the presence of tobacco consumption; for the condensed-chromatin cells, our model showed that the exposure to welding-fumes is the only reliable predictor.Further studies should shed more light on this subject.

View Article: PubMed Central - PubMed

Affiliation: Faculty of medicine, Universidad Autónoma de Guadalajara, Zapopan, Jalisco, Mexico.

ABSTRACT

Background: An estimated 800,000 people worldwide are occupationally exposed to welding-fumes. Previous studies show that the exposure to such fumes is associated with damage to genetic material and increased cancer risk. In this study, we evaluate the genotoxic effect of welding-fumes using the Micronucleus Test on oral mucosa cells of Mexican welders.

Material and methods: We conducted a cross-sectional, matched case-control study of n = 66 (33 exposed welders, and 33 healthy controls). Buccal mucosa smears were collected and stained with acridine orange, observed under 100x optical amplification with a fluorescence lamp, and a single-blinded observer counted the number of micronuclei and other nuclear abnormalities per 2,000 observed cells. We compared the frequencies of micronuclei and other nuclear abnormalities, and fitted generalised linear models to investigate the interactions between nuclear abnormalities and the exposure to welding-fumes, while controlling for smoking and age.

Results: Binucleated cells and condensed-chromatin cells showed statistically significant differences between cases and controls. The frequency of micronuclei and the rest of nuclear abnormalities (lobed-nuclei, pyknosis, karyolysis, and karyorrhexis) did not differ significantly between the groups. After adjusting for smoking, the regression results showed that the occurrence of binucleated cells could be predicted by the exposure to welding-fumes plus the presence of tobacco consumption; for the condensed-chromatin cells, our model showed that the exposure to welding-fumes is the only reliable predictor.

Conclusions: Our findings suggest that Mexican welders who are occupationally exposed to welding-fumes have increased counts of binucleated and condensed-chromatin cells. Nevertheless, the frequencies of micronuclei and the rest of nuclear abnormalities did not differ between cases and controls. Further studies should shed more light on this subject.

No MeSH data available.


Related in: MedlinePlus