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Description of the Microsporidian Parasite, Heterosporis sutherlandae n. sp., Infecting Fish in the Great Lakes Region, USA.

Phelps NB, Mor SK, Armién AG, Pelican KM, Goyal SM - PLoS ONE (2015)

Bottom Line: A unique species of Heterosporis was identified, demonstrating less than 96% sequence identity to other published Heterosporis sp. on the basis of partial rRNA gene sequence analysis.Heterosporis sutherlandae n. sp. (formerly Heterosporis sp.) was identified in yellow perch (Perca flavescens), northern pike (Esox lucius) and walleye (Sander vitreus) from inland lakes in Minnesota and Wisconsin.Previous research suggests this species may be even more widespread in the Great Lakes region and should be reexamined using molecular techniques to better understand the distribution of this novel species.

View Article: PubMed Central - PubMed

Affiliation: Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, 1333 Gortner Avenue, Saint Paul, Minnesota, 55108, United States of America; Veterinary Population Medicine Department, College of Veterinary Medicine, University of Minnesota, 1365 Gortner Avenue, Saint Paul, Minnesota, 55108, United States of America.

ABSTRACT
Heterosporosis is an increasingly important microsporidian disease worldwide, impacting wild and farmed raised fishes in both marine and freshwater environments. A previously undescribed species (Heterosporis sp.), with widespread distribution in the Great Lakes region, was the subject of this study. Three angler-caught fish were submitted to the Minnesota Veterinary Diagnostic Laboratory from 2009-2010 with lesions caused by intracellular proliferation of parasitic spores, resulting in destruction and eventual widespread necrosis of the host skeletal muscles. Mature ovoid (5.8 x 3.5 μm) spores of a microsporidian parasite, consistent with the genus Heterosporis, were observed by light and electron microscopy. Molecular identification was performed using primer walking to obtain a near-complete rRNA gene sequence (~3,600 bp). A unique species of Heterosporis was identified, demonstrating less than 96% sequence identity to other published Heterosporis sp. on the basis of partial rRNA gene sequence analysis. Heterosporis sutherlandae n. sp. (formerly Heterosporis sp.) was identified in yellow perch (Perca flavescens), northern pike (Esox lucius) and walleye (Sander vitreus) from inland lakes in Minnesota and Wisconsin. Previous research suggests this species may be even more widespread in the Great Lakes region and should be reexamined using molecular techniques to better understand the distribution of this novel species.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic analysis based on a 1,200 bp nucleotide sequence, corresponding to nucleotide positions 600 to 1,800 of reference sequence AF387331.The Maximum Likelihood phylogenetic tree was constructed using the General Time Reversible (GTR) model of nucleotide substitution (4 gamma categories) model with 1,000 bootstrap replicates.
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pone.0132027.g006: Phylogenetic analysis based on a 1,200 bp nucleotide sequence, corresponding to nucleotide positions 600 to 1,800 of reference sequence AF387331.The Maximum Likelihood phylogenetic tree was constructed using the General Time Reversible (GTR) model of nucleotide substitution (4 gamma categories) model with 1,000 bootstrap replicates.

Mentions: Most of the fish-infecting microsporidian sequences in GenBank are partial sequences of the SSU, complete ITS, and partial LSU of the rRNA gene. Therefore, additional phylogenetic analysis was performed on a 1,200 nucleotide segment (nucleotide position 600 to 1,800 of H. anguillarum). Based on this phylogenetic analysis, Heterosporis species were divided into two subgroups (I and II) within group 3 of the fish-infecting microsporidia (Fig 6). Subgroup I included six sequences from this study (Heterosporis sp.), as well as the previously unclassified Heterosporis sp. (AF356225) first reported in Wisconsin [20]. Subgroup II included H. saurida (JF745533) and H. anguillarum (AF387331). Subgroup I sequences have 98.5%–100% sequence identity within the subgroup and 92.4%–96.0% sequence identity with subgroup II (S2 Table). Subgroup I was also closely related to Ovipleistophora mirandellae (AF356223), Ovipleistophora ovariae (AJ252955), and Pleistophora hyphessobryconis (GU12667) having 93.8–94.1%, 92.1–94.1%, and 94.5%-94.8% sequence identity, respectively (S2 Table).


Description of the Microsporidian Parasite, Heterosporis sutherlandae n. sp., Infecting Fish in the Great Lakes Region, USA.

Phelps NB, Mor SK, Armién AG, Pelican KM, Goyal SM - PLoS ONE (2015)

Phylogenetic analysis based on a 1,200 bp nucleotide sequence, corresponding to nucleotide positions 600 to 1,800 of reference sequence AF387331.The Maximum Likelihood phylogenetic tree was constructed using the General Time Reversible (GTR) model of nucleotide substitution (4 gamma categories) model with 1,000 bootstrap replicates.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526549&req=5

pone.0132027.g006: Phylogenetic analysis based on a 1,200 bp nucleotide sequence, corresponding to nucleotide positions 600 to 1,800 of reference sequence AF387331.The Maximum Likelihood phylogenetic tree was constructed using the General Time Reversible (GTR) model of nucleotide substitution (4 gamma categories) model with 1,000 bootstrap replicates.
Mentions: Most of the fish-infecting microsporidian sequences in GenBank are partial sequences of the SSU, complete ITS, and partial LSU of the rRNA gene. Therefore, additional phylogenetic analysis was performed on a 1,200 nucleotide segment (nucleotide position 600 to 1,800 of H. anguillarum). Based on this phylogenetic analysis, Heterosporis species were divided into two subgroups (I and II) within group 3 of the fish-infecting microsporidia (Fig 6). Subgroup I included six sequences from this study (Heterosporis sp.), as well as the previously unclassified Heterosporis sp. (AF356225) first reported in Wisconsin [20]. Subgroup II included H. saurida (JF745533) and H. anguillarum (AF387331). Subgroup I sequences have 98.5%–100% sequence identity within the subgroup and 92.4%–96.0% sequence identity with subgroup II (S2 Table). Subgroup I was also closely related to Ovipleistophora mirandellae (AF356223), Ovipleistophora ovariae (AJ252955), and Pleistophora hyphessobryconis (GU12667) having 93.8–94.1%, 92.1–94.1%, and 94.5%-94.8% sequence identity, respectively (S2 Table).

Bottom Line: A unique species of Heterosporis was identified, demonstrating less than 96% sequence identity to other published Heterosporis sp. on the basis of partial rRNA gene sequence analysis.Heterosporis sutherlandae n. sp. (formerly Heterosporis sp.) was identified in yellow perch (Perca flavescens), northern pike (Esox lucius) and walleye (Sander vitreus) from inland lakes in Minnesota and Wisconsin.Previous research suggests this species may be even more widespread in the Great Lakes region and should be reexamined using molecular techniques to better understand the distribution of this novel species.

View Article: PubMed Central - PubMed

Affiliation: Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, 1333 Gortner Avenue, Saint Paul, Minnesota, 55108, United States of America; Veterinary Population Medicine Department, College of Veterinary Medicine, University of Minnesota, 1365 Gortner Avenue, Saint Paul, Minnesota, 55108, United States of America.

ABSTRACT
Heterosporosis is an increasingly important microsporidian disease worldwide, impacting wild and farmed raised fishes in both marine and freshwater environments. A previously undescribed species (Heterosporis sp.), with widespread distribution in the Great Lakes region, was the subject of this study. Three angler-caught fish were submitted to the Minnesota Veterinary Diagnostic Laboratory from 2009-2010 with lesions caused by intracellular proliferation of parasitic spores, resulting in destruction and eventual widespread necrosis of the host skeletal muscles. Mature ovoid (5.8 x 3.5 μm) spores of a microsporidian parasite, consistent with the genus Heterosporis, were observed by light and electron microscopy. Molecular identification was performed using primer walking to obtain a near-complete rRNA gene sequence (~3,600 bp). A unique species of Heterosporis was identified, demonstrating less than 96% sequence identity to other published Heterosporis sp. on the basis of partial rRNA gene sequence analysis. Heterosporis sutherlandae n. sp. (formerly Heterosporis sp.) was identified in yellow perch (Perca flavescens), northern pike (Esox lucius) and walleye (Sander vitreus) from inland lakes in Minnesota and Wisconsin. Previous research suggests this species may be even more widespread in the Great Lakes region and should be reexamined using molecular techniques to better understand the distribution of this novel species.

No MeSH data available.


Related in: MedlinePlus