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Comparison of Leaf Sheath Transcriptome Profiles with Physiological Traits of Bread Wheat Cultivars under Salinity Stress.

Takahashi F, Tilbrook J, Trittermann C, Berger B, Roy SJ, Seki M, Shinozaki K, Tester M - PLoS ONE (2015)

Bottom Line: Early responses to mild salinity stress through changes in gene expression have an influence on the acquisition of stress tolerance and improvement in biomass accumulation during the early "osmotic" phase of salinity stress.In addition, results revealed transcript profiles for the wheat cultivars that were different from those of usual stress-inducible genes, but were related to those of plant growth.These findings suggest that, in the process of breeding, selection of specific traits with various salinity stress-inducible genes in commercial bread wheat has led to adaptation to mild salinity conditions.

View Article: PubMed Central - PubMed

Affiliation: Biomass Research Platform Team, RIKEN Center for Sustainable Resource Science, Koyadai, Tsukuba, Ibaraki, Japan; Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science, Koyadai, Tsukuba, Ibaraki, Japan; The Plant Accelerator, Australian Plant Phenomics Facility, School of Agriculture, Food & Wine, University of Adelaide, Glen Osmond, Australia.

ABSTRACT
Salinity stress has significant negative effects on plant biomass production and crop yield. Salinity tolerance is controlled by complex systems of gene expression and ion transport. The relationship between specific features of mild salinity stress adaptation and gene expression was analyzed using four commercial varieties of bread wheat (Triticum aestivum) that have different levels of salinity tolerance. The high-throughput phenotyping system in The Plant Accelerator at the Australian Plant Phenomics Facility revealed variation in shoot relative growth rate and salinity tolerance among the four cultivars. Comparative analysis of gene expression in the leaf sheaths identified genes whose functions are potentially linked to shoot biomass development and salinity tolerance. Early responses to mild salinity stress through changes in gene expression have an influence on the acquisition of stress tolerance and improvement in biomass accumulation during the early "osmotic" phase of salinity stress. In addition, results revealed transcript profiles for the wheat cultivars that were different from those of usual stress-inducible genes, but were related to those of plant growth. These findings suggest that, in the process of breeding, selection of specific traits with various salinity stress-inducible genes in commercial bread wheat has led to adaptation to mild salinity conditions.

No MeSH data available.


Related in: MedlinePlus

Gene expression pattern for four cultivars under conditions of salinity stress.(A) Venn diagrams showing a five-fold or greater difference in expression with all possible regressions under saline conditions (100 mM NaCl). Red numeric characters show the number of upregulated genes. Blue numeric characters show the number of downregulated genes. (B) Analysis of the gene ontology of 48 genes increased only in Krichauff under saline conditions. Functional categorizations by annotation were shown as gene ontology of biological process. (C) Analysis of the gene ontology of 21 genes increased only in Drysdale under saline conditions.
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pone.0133322.g005: Gene expression pattern for four cultivars under conditions of salinity stress.(A) Venn diagrams showing a five-fold or greater difference in expression with all possible regressions under saline conditions (100 mM NaCl). Red numeric characters show the number of upregulated genes. Blue numeric characters show the number of downregulated genes. (B) Analysis of the gene ontology of 48 genes increased only in Krichauff under saline conditions. Functional categorizations by annotation were shown as gene ontology of biological process. (C) Analysis of the gene ontology of 21 genes increased only in Drysdale under saline conditions.

Mentions: Drysdale and Krichauff showed higher STI with the 100 mM NaCl treatment in comparison with Gladius and Berkut (Table 1), and they showed specific temporal variations in gene expression in response to salinity stress (Fig 4). Most upregulated and downregulated genes were expressed within 24 h of salinity treatment in both cultivars (Fig 4A). Of the 47 genes in Drysdale and 96 genes in Krichauff that were upregulated under salinity stress compared with control (RO water) conditions, 42 (89.4%) and 91 (94.8%), respectively, were observed to be upregulated one day after treatment. However, only five genes were similarly regulated in both cultivars (Fig 5A and Table 3). In contrast, the more salt sensitive Gladius and Berkut varieties did not exhibit dramatic changes in the numbers of upregulated genes during the three days after stress treatment, with only about 10 genes upregulated by mild salinity stress in each of the varieties. The same tendency was observed in the gene sets which were downregulated under mild salinity stress conditions (Figs 4 and 5A and S2 Table).


Comparison of Leaf Sheath Transcriptome Profiles with Physiological Traits of Bread Wheat Cultivars under Salinity Stress.

Takahashi F, Tilbrook J, Trittermann C, Berger B, Roy SJ, Seki M, Shinozaki K, Tester M - PLoS ONE (2015)

Gene expression pattern for four cultivars under conditions of salinity stress.(A) Venn diagrams showing a five-fold or greater difference in expression with all possible regressions under saline conditions (100 mM NaCl). Red numeric characters show the number of upregulated genes. Blue numeric characters show the number of downregulated genes. (B) Analysis of the gene ontology of 48 genes increased only in Krichauff under saline conditions. Functional categorizations by annotation were shown as gene ontology of biological process. (C) Analysis of the gene ontology of 21 genes increased only in Drysdale under saline conditions.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526543&req=5

pone.0133322.g005: Gene expression pattern for four cultivars under conditions of salinity stress.(A) Venn diagrams showing a five-fold or greater difference in expression with all possible regressions under saline conditions (100 mM NaCl). Red numeric characters show the number of upregulated genes. Blue numeric characters show the number of downregulated genes. (B) Analysis of the gene ontology of 48 genes increased only in Krichauff under saline conditions. Functional categorizations by annotation were shown as gene ontology of biological process. (C) Analysis of the gene ontology of 21 genes increased only in Drysdale under saline conditions.
Mentions: Drysdale and Krichauff showed higher STI with the 100 mM NaCl treatment in comparison with Gladius and Berkut (Table 1), and they showed specific temporal variations in gene expression in response to salinity stress (Fig 4). Most upregulated and downregulated genes were expressed within 24 h of salinity treatment in both cultivars (Fig 4A). Of the 47 genes in Drysdale and 96 genes in Krichauff that were upregulated under salinity stress compared with control (RO water) conditions, 42 (89.4%) and 91 (94.8%), respectively, were observed to be upregulated one day after treatment. However, only five genes were similarly regulated in both cultivars (Fig 5A and Table 3). In contrast, the more salt sensitive Gladius and Berkut varieties did not exhibit dramatic changes in the numbers of upregulated genes during the three days after stress treatment, with only about 10 genes upregulated by mild salinity stress in each of the varieties. The same tendency was observed in the gene sets which were downregulated under mild salinity stress conditions (Figs 4 and 5A and S2 Table).

Bottom Line: Early responses to mild salinity stress through changes in gene expression have an influence on the acquisition of stress tolerance and improvement in biomass accumulation during the early "osmotic" phase of salinity stress.In addition, results revealed transcript profiles for the wheat cultivars that were different from those of usual stress-inducible genes, but were related to those of plant growth.These findings suggest that, in the process of breeding, selection of specific traits with various salinity stress-inducible genes in commercial bread wheat has led to adaptation to mild salinity conditions.

View Article: PubMed Central - PubMed

Affiliation: Biomass Research Platform Team, RIKEN Center for Sustainable Resource Science, Koyadai, Tsukuba, Ibaraki, Japan; Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science, Koyadai, Tsukuba, Ibaraki, Japan; The Plant Accelerator, Australian Plant Phenomics Facility, School of Agriculture, Food & Wine, University of Adelaide, Glen Osmond, Australia.

ABSTRACT
Salinity stress has significant negative effects on plant biomass production and crop yield. Salinity tolerance is controlled by complex systems of gene expression and ion transport. The relationship between specific features of mild salinity stress adaptation and gene expression was analyzed using four commercial varieties of bread wheat (Triticum aestivum) that have different levels of salinity tolerance. The high-throughput phenotyping system in The Plant Accelerator at the Australian Plant Phenomics Facility revealed variation in shoot relative growth rate and salinity tolerance among the four cultivars. Comparative analysis of gene expression in the leaf sheaths identified genes whose functions are potentially linked to shoot biomass development and salinity tolerance. Early responses to mild salinity stress through changes in gene expression have an influence on the acquisition of stress tolerance and improvement in biomass accumulation during the early "osmotic" phase of salinity stress. In addition, results revealed transcript profiles for the wheat cultivars that were different from those of usual stress-inducible genes, but were related to those of plant growth. These findings suggest that, in the process of breeding, selection of specific traits with various salinity stress-inducible genes in commercial bread wheat has led to adaptation to mild salinity conditions.

No MeSH data available.


Related in: MedlinePlus