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Comparison of Leaf Sheath Transcriptome Profiles with Physiological Traits of Bread Wheat Cultivars under Salinity Stress.

Takahashi F, Tilbrook J, Trittermann C, Berger B, Roy SJ, Seki M, Shinozaki K, Tester M - PLoS ONE (2015)

Bottom Line: Early responses to mild salinity stress through changes in gene expression have an influence on the acquisition of stress tolerance and improvement in biomass accumulation during the early "osmotic" phase of salinity stress.In addition, results revealed transcript profiles for the wheat cultivars that were different from those of usual stress-inducible genes, but were related to those of plant growth.These findings suggest that, in the process of breeding, selection of specific traits with various salinity stress-inducible genes in commercial bread wheat has led to adaptation to mild salinity conditions.

View Article: PubMed Central - PubMed

Affiliation: Biomass Research Platform Team, RIKEN Center for Sustainable Resource Science, Koyadai, Tsukuba, Ibaraki, Japan; Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science, Koyadai, Tsukuba, Ibaraki, Japan; The Plant Accelerator, Australian Plant Phenomics Facility, School of Agriculture, Food & Wine, University of Adelaide, Glen Osmond, Australia.

ABSTRACT
Salinity stress has significant negative effects on plant biomass production and crop yield. Salinity tolerance is controlled by complex systems of gene expression and ion transport. The relationship between specific features of mild salinity stress adaptation and gene expression was analyzed using four commercial varieties of bread wheat (Triticum aestivum) that have different levels of salinity tolerance. The high-throughput phenotyping system in The Plant Accelerator at the Australian Plant Phenomics Facility revealed variation in shoot relative growth rate and salinity tolerance among the four cultivars. Comparative analysis of gene expression in the leaf sheaths identified genes whose functions are potentially linked to shoot biomass development and salinity tolerance. Early responses to mild salinity stress through changes in gene expression have an influence on the acquisition of stress tolerance and improvement in biomass accumulation during the early "osmotic" phase of salinity stress. In addition, results revealed transcript profiles for the wheat cultivars that were different from those of usual stress-inducible genes, but were related to those of plant growth. These findings suggest that, in the process of breeding, selection of specific traits with various salinity stress-inducible genes in commercial bread wheat has led to adaptation to mild salinity conditions.

No MeSH data available.


Related in: MedlinePlus

Gene expression pattern for the four cultivars under control conditions.(A) Venn diagrams showing a five-fold or greater difference in expression with all possible regressions during the first 3 days of growth under control conditions compared with the starting point (day 0) of the experiments. (B) Heat maps indicating intensity of gene expression in Berkut. Log FC = log2 (the signal intensity under saline conditions / the signal intensity under control conditions). (C) Analysis of the gene ontology of 39 genes upregulated only in Berkut under control conditions. Functional categorizations by annotation were shown as gene ontology of biological process.
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pone.0133322.g003: Gene expression pattern for the four cultivars under control conditions.(A) Venn diagrams showing a five-fold or greater difference in expression with all possible regressions during the first 3 days of growth under control conditions compared with the starting point (day 0) of the experiments. (B) Heat maps indicating intensity of gene expression in Berkut. Log FC = log2 (the signal intensity under saline conditions / the signal intensity under control conditions). (C) Analysis of the gene ontology of 39 genes upregulated only in Berkut under control conditions. Functional categorizations by annotation were shown as gene ontology of biological process.

Mentions: Although the patterns of leaf sheath transcriptome regulation varied sharply among the cultivars, an attempt was made to identify common responsive genes governing shoot biomass development and salinity tolerance. We focused first on RGRs under control conditions. Berkut showed the highest mean RGR under control conditions in both experiments (Fig 2 and S3 Fig). Comparative gene expression analysis revealed that 39 genes were upregulated only in Berkut during the first 3 days of growth under control conditions compared with the starting point (day 0) of the experiments (Fig 3A).


Comparison of Leaf Sheath Transcriptome Profiles with Physiological Traits of Bread Wheat Cultivars under Salinity Stress.

Takahashi F, Tilbrook J, Trittermann C, Berger B, Roy SJ, Seki M, Shinozaki K, Tester M - PLoS ONE (2015)

Gene expression pattern for the four cultivars under control conditions.(A) Venn diagrams showing a five-fold or greater difference in expression with all possible regressions during the first 3 days of growth under control conditions compared with the starting point (day 0) of the experiments. (B) Heat maps indicating intensity of gene expression in Berkut. Log FC = log2 (the signal intensity under saline conditions / the signal intensity under control conditions). (C) Analysis of the gene ontology of 39 genes upregulated only in Berkut under control conditions. Functional categorizations by annotation were shown as gene ontology of biological process.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526543&req=5

pone.0133322.g003: Gene expression pattern for the four cultivars under control conditions.(A) Venn diagrams showing a five-fold or greater difference in expression with all possible regressions during the first 3 days of growth under control conditions compared with the starting point (day 0) of the experiments. (B) Heat maps indicating intensity of gene expression in Berkut. Log FC = log2 (the signal intensity under saline conditions / the signal intensity under control conditions). (C) Analysis of the gene ontology of 39 genes upregulated only in Berkut under control conditions. Functional categorizations by annotation were shown as gene ontology of biological process.
Mentions: Although the patterns of leaf sheath transcriptome regulation varied sharply among the cultivars, an attempt was made to identify common responsive genes governing shoot biomass development and salinity tolerance. We focused first on RGRs under control conditions. Berkut showed the highest mean RGR under control conditions in both experiments (Fig 2 and S3 Fig). Comparative gene expression analysis revealed that 39 genes were upregulated only in Berkut during the first 3 days of growth under control conditions compared with the starting point (day 0) of the experiments (Fig 3A).

Bottom Line: Early responses to mild salinity stress through changes in gene expression have an influence on the acquisition of stress tolerance and improvement in biomass accumulation during the early "osmotic" phase of salinity stress.In addition, results revealed transcript profiles for the wheat cultivars that were different from those of usual stress-inducible genes, but were related to those of plant growth.These findings suggest that, in the process of breeding, selection of specific traits with various salinity stress-inducible genes in commercial bread wheat has led to adaptation to mild salinity conditions.

View Article: PubMed Central - PubMed

Affiliation: Biomass Research Platform Team, RIKEN Center for Sustainable Resource Science, Koyadai, Tsukuba, Ibaraki, Japan; Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science, Koyadai, Tsukuba, Ibaraki, Japan; The Plant Accelerator, Australian Plant Phenomics Facility, School of Agriculture, Food & Wine, University of Adelaide, Glen Osmond, Australia.

ABSTRACT
Salinity stress has significant negative effects on plant biomass production and crop yield. Salinity tolerance is controlled by complex systems of gene expression and ion transport. The relationship between specific features of mild salinity stress adaptation and gene expression was analyzed using four commercial varieties of bread wheat (Triticum aestivum) that have different levels of salinity tolerance. The high-throughput phenotyping system in The Plant Accelerator at the Australian Plant Phenomics Facility revealed variation in shoot relative growth rate and salinity tolerance among the four cultivars. Comparative analysis of gene expression in the leaf sheaths identified genes whose functions are potentially linked to shoot biomass development and salinity tolerance. Early responses to mild salinity stress through changes in gene expression have an influence on the acquisition of stress tolerance and improvement in biomass accumulation during the early "osmotic" phase of salinity stress. In addition, results revealed transcript profiles for the wheat cultivars that were different from those of usual stress-inducible genes, but were related to those of plant growth. These findings suggest that, in the process of breeding, selection of specific traits with various salinity stress-inducible genes in commercial bread wheat has led to adaptation to mild salinity conditions.

No MeSH data available.


Related in: MedlinePlus